Phase-contrast enhanced synchrotron micro-tomography of human meniscus tissue
(2022) In Osteoarthritis and Cartilage 30(9). p.1222-1233- Abstract
OBJECTIVE: To investigate the feasibility of synchrotron radiation-based phase contrast enhanced micro-computed tomography (SR-PhC-μCT) for imaging of human meniscus. Quantitative parameters related to fiber orientation and crimping were evaluated as potential markers of tissue degeneration.
DESIGN: Human meniscus specimens from 10 deceased donors were prepared using different preparation schemes: fresh frozen and thawed before imaging or fixed and paraffin-embedded. The samples were imaged using SR-PhC-μCT with an isotropic voxel size of 1.625 μm. Image quality was evaluated by visual inspection and spatial resolution. Fiber voxels were defined using a grey level threshold and a structure tensor analysis was applied to estimate... (More)
OBJECTIVE: To investigate the feasibility of synchrotron radiation-based phase contrast enhanced micro-computed tomography (SR-PhC-μCT) for imaging of human meniscus. Quantitative parameters related to fiber orientation and crimping were evaluated as potential markers of tissue degeneration.
DESIGN: Human meniscus specimens from 10 deceased donors were prepared using different preparation schemes: fresh frozen and thawed before imaging or fixed and paraffin-embedded. The samples were imaged using SR-PhC-μCT with an isotropic voxel size of 1.625 μm. Image quality was evaluated by visual inspection and spatial resolution. Fiber voxels were defined using a grey level threshold and a structure tensor analysis was applied to estimate collagen fiber orientation. The area at half maximum (FAHM) was calculated from angle histograms to quantify orientation distribution. Crimping period was calculated from the power spectrum of image profiles of crimped fibers. Parameters were compared to degenerative stage as evaluated by Pauli histopathological scoring.
RESULTS: Image quality was similar between frozen and embedded samples and spatial resolutions ranged from 5.1 to 5.8 μm. Fiber structure, including crimping, was clearly visible in the images. Fibers appeared to be less organized closer to the tip of the meniscus. Fiber density might decrease slightly with degeneration. FAHM and crimping period did not show any clear association with histopathological scoring.
CONCLUSION: SR-PhC-μCT is a feasible technique for high-resolution 3D imaging of fresh frozen meniscus tissue. Further work is needed to establish quantitative parameters that relate to tissue degeneration, but this imaging technique is promising for future studies of meniscus structure and biomechanical response.
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- author
- Einarsson, E LU ; Pierantoni, M LU ; Novak, V ; Svensson, J LU ; Isaksson, H LU and Englund, M LU
- organization
-
- Medical Radiation Physics, Malmö (research group)
- Department of Translational Medicine
- Lund University Bioimaging Center
- LTH Profile Area: Engineering Health
- Department of Biomedical Engineering
- Lund OsteoArthritis Division - Clinical Epidemiology Unit (research group)
- CPUP - Children's Orthopedics Group (research group)
- Department of Clinical Sciences, Lund
- publishing date
- 2022-09
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Humans, Meniscus, Microscopy, Phase-Contrast, Synchrotrons, Tomography, X-Ray Microtomography
- in
- Osteoarthritis and Cartilage
- volume
- 30
- issue
- 9
- pages
- 12 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85134814472
- pmid:35750240
- ISSN
- 1063-4584
- DOI
- 10.1016/j.joca.2022.06.003
- language
- English
- LU publication?
- yes
- additional info
- Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.
- id
- 6f9d73bd-404d-4e44-abf6-f8ee599d2d12
- date added to LUP
- 2023-10-19 15:47:01
- date last changed
- 2024-04-19 02:38:43
@article{6f9d73bd-404d-4e44-abf6-f8ee599d2d12, abstract = {{<p>OBJECTIVE: To investigate the feasibility of synchrotron radiation-based phase contrast enhanced micro-computed tomography (SR-PhC-μCT) for imaging of human meniscus. Quantitative parameters related to fiber orientation and crimping were evaluated as potential markers of tissue degeneration.</p><p>DESIGN: Human meniscus specimens from 10 deceased donors were prepared using different preparation schemes: fresh frozen and thawed before imaging or fixed and paraffin-embedded. The samples were imaged using SR-PhC-μCT with an isotropic voxel size of 1.625 μm. Image quality was evaluated by visual inspection and spatial resolution. Fiber voxels were defined using a grey level threshold and a structure tensor analysis was applied to estimate collagen fiber orientation. The area at half maximum (FAHM) was calculated from angle histograms to quantify orientation distribution. Crimping period was calculated from the power spectrum of image profiles of crimped fibers. Parameters were compared to degenerative stage as evaluated by Pauli histopathological scoring.</p><p>RESULTS: Image quality was similar between frozen and embedded samples and spatial resolutions ranged from 5.1 to 5.8 μm. Fiber structure, including crimping, was clearly visible in the images. Fibers appeared to be less organized closer to the tip of the meniscus. Fiber density might decrease slightly with degeneration. FAHM and crimping period did not show any clear association with histopathological scoring.</p><p>CONCLUSION: SR-PhC-μCT is a feasible technique for high-resolution 3D imaging of fresh frozen meniscus tissue. Further work is needed to establish quantitative parameters that relate to tissue degeneration, but this imaging technique is promising for future studies of meniscus structure and biomechanical response.</p>}}, author = {{Einarsson, E and Pierantoni, M and Novak, V and Svensson, J and Isaksson, H and Englund, M}}, issn = {{1063-4584}}, keywords = {{Humans; Meniscus; Microscopy, Phase-Contrast; Synchrotrons; Tomography; X-Ray Microtomography}}, language = {{eng}}, number = {{9}}, pages = {{1222--1233}}, publisher = {{Elsevier}}, series = {{Osteoarthritis and Cartilage}}, title = {{Phase-contrast enhanced synchrotron micro-tomography of human meniscus tissue}}, url = {{http://dx.doi.org/10.1016/j.joca.2022.06.003}}, doi = {{10.1016/j.joca.2022.06.003}}, volume = {{30}}, year = {{2022}}, }