Brassica oleracea L. var. italica Aquaporin Reconstituted Proteoliposomes as Nanosystems for Resveratrol Encapsulation
Yepes-Molina, Lucia LU ; Teruel, José A. ; Johanson, Urban LU
and Carvajal, Micaela
(2024)
In International Journal of Molecular Sciences
25(4).
- Abstract
Aquaporins (AQPs), membrane proteins responsible for facilitating water transport, found in plant membrane vesicles (MV), have been related to the functionality and stability of MV. We focused on AQPs obtained from broccoli, as they show potential for biotechnological applications. To gain further insight into the role of AQPs in MV, we describe the heterologous overexpression of two broccoli AQPs ( BoPIP1;2 and BoPIP2;2) in Pichia pastoris, resulting in their purification with high yield (0.14 and 0.99 mg per gram cells for BoPIP1;2 and BoPIP2;2). We reconstituted AQPs in liposomes to study their functionality, and the size of proteoliposomes did not change concerning liposomes. BoPIP2;2 facilitated water transport, which was... (More)
Aquaporins (AQPs), membrane proteins responsible for facilitating water transport, found in plant membrane vesicles (MV), have been related to the functionality and stability of MV. We focused on AQPs obtained from broccoli, as they show potential for biotechnological applications. To gain further insight into the role of AQPs in MV, we describe the heterologous overexpression of two broccoli AQPs ( BoPIP1;2 and BoPIP2;2) in Pichia pastoris, resulting in their purification with high yield (0.14 and 0.99 mg per gram cells for BoPIP1;2 and BoPIP2;2). We reconstituted AQPs in liposomes to study their functionality, and the size of proteoliposomes did not change concerning liposomes. BoPIP2;2 facilitated water transport, which was preserved for seven days at 4 °C and at room temperature but not at 37 °C. BoPIP2;2 was incorporated into liposomes to encapsulate a resveratrol extract, resulting in increased entrapment efficiency (EE) compared to conventional liposomes. Molecular docking was utilized to identify binding sites in PIP2s for resveratrol, highlighting the role of aquaporins in the improved EE. Moreover, interactions between plant AQP and human integrin were shown, which may increase internalization by the human target cells. Our results suggest AQP-based alternative encapsulation systems can be used in specifically targeted biotechnological applications.
(Less)
- author
- Yepes-Molina, Lucia
LU
; Teruel, José A.
; Johanson, Urban
LU
and Carvajal, Micaela
- organization
- publishing date
- 2024-02-06
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Humans, Liposomes/metabolism, Resveratrol/metabolism, Molecular Docking Simulation, Aquaporins/metabolism, Brassica/genetics, Water/chemistry, Proteolipids
- in
- International Journal of Molecular Sciences
- volume
- 25
- issue
- 4
- article number
- 1987
- pages
- 15 pages
- publisher
- MDPI AG
- external identifiers
-
- scopus:85185900434
- pmid:38396666
- ISSN
- 1422-0067
- DOI
- 10.3390/ijms25041987
- language
- English
- LU publication?
- yes
- id
- 6fbdf6ab-0957-4c66-b5b0-10c89adfb309
- date added to LUP
- 2024-03-11 09:36:34
- date last changed
- 2024-04-23 14:30:59
@article{6fbdf6ab-0957-4c66-b5b0-10c89adfb309,
abstract = {{<p>Aquaporins (AQPs), membrane proteins responsible for facilitating water transport, found in plant membrane vesicles (MV), have been related to the functionality and stability of MV. We focused on AQPs obtained from broccoli, as they show potential for biotechnological applications. To gain further insight into the role of AQPs in MV, we describe the heterologous overexpression of two broccoli AQPs ( BoPIP1;2 and BoPIP2;2) in Pichia pastoris, resulting in their purification with high yield (0.14 and 0.99 mg per gram cells for BoPIP1;2 and BoPIP2;2). We reconstituted AQPs in liposomes to study their functionality, and the size of proteoliposomes did not change concerning liposomes. BoPIP2;2 facilitated water transport, which was preserved for seven days at 4 °C and at room temperature but not at 37 °C. BoPIP2;2 was incorporated into liposomes to encapsulate a resveratrol extract, resulting in increased entrapment efficiency (EE) compared to conventional liposomes. Molecular docking was utilized to identify binding sites in PIP2s for resveratrol, highlighting the role of aquaporins in the improved EE. Moreover, interactions between plant AQP and human integrin were shown, which may increase internalization by the human target cells. Our results suggest AQP-based alternative encapsulation systems can be used in specifically targeted biotechnological applications. </p>}},
author = {{Yepes-Molina, Lucia and Teruel, José A. and Johanson, Urban and Carvajal, Micaela}},
issn = {{1422-0067}},
keywords = {{Humans; Liposomes/metabolism; Resveratrol/metabolism; Molecular Docking Simulation; Aquaporins/metabolism; Brassica/genetics; Water/chemistry; Proteolipids}},
language = {{eng}},
month = {{02}},
number = {{4}},
publisher = {{MDPI AG}},
series = {{International Journal of Molecular Sciences}},
title = {{<i>Brassica oleracea </i>L. var. <i>italica </i>Aquaporin Reconstituted Proteoliposomes as Nanosystems for Resveratrol Encapsulation}},
url = {{http://dx.doi.org/10.3390/ijms25041987}},
doi = {{10.3390/ijms25041987}},
volume = {{25}},
year = {{2024}},
}