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HC-IgA antibodies of different specificities are normally present in serum : quantitation by ELISA and relationship to the major Ig classes

Truedsson, Lennart LU ; Karypidis, Anna-Helena ; Eberhardt, Kerstin LU and Grubb, Anders LU orcid (1990) In APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 98(1-6). p.249-254
Abstract

Enzyme-linked immunosorbent assays (ELISA) were developed for direct measurement of protein HC-IgA complexes (HC-IgA) in serum with antibody specificity for rabbit IgG (rheumatoid factor (RF) activity), lipopolysaccharide from Yersinia enterocolitica serotype O:3 (Y3) and tetanus toxoid (TT). About 80% of patients with rheumatoid arthritis had increased concentrations of HC-IgA-RF. The values were correlated with the concentrations of IgA-RF and IgM-RF. HC-IgA anti-Y3 was measured in 45 sera with anti-Y3 antibodies of IgM, IgG and IgA class. The HC-IgA anti-Y3 levels were correlated with those of anti-Y3 of IgG and IgM class, but not of IgA class. For HC-IgA anti-Y3, the closest correlation was that with the specific IgM antibody... (More)

Enzyme-linked immunosorbent assays (ELISA) were developed for direct measurement of protein HC-IgA complexes (HC-IgA) in serum with antibody specificity for rabbit IgG (rheumatoid factor (RF) activity), lipopolysaccharide from Yersinia enterocolitica serotype O:3 (Y3) and tetanus toxoid (TT). About 80% of patients with rheumatoid arthritis had increased concentrations of HC-IgA-RF. The values were correlated with the concentrations of IgA-RF and IgM-RF. HC-IgA anti-Y3 was measured in 45 sera with anti-Y3 antibodies of IgM, IgG and IgA class. The HC-IgA anti-Y3 levels were correlated with those of anti-Y3 of IgG and IgM class, but not of IgA class. For HC-IgA anti-Y3, the closest correlation was that with the specific IgM antibody concentration, rs = 0.63 (p less than 0.001). In 25 normal sera, significant correlations were observed between HC-IgA anti-TT and specific antibodies of IgG and IgA class, but not of IgM class. In 107 sera containing IgA M-components, the total concentration of HC-IgA correlated poorly with both protein HC and with IgA concentrations. It was concluded that specific HC-IgA antibodies are normal constituents of serum, and that their concentrations are not directly related to the serum content of specific IgA antibodies.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alpha-Globulins/analysis, Enzyme-Linked Immunosorbent Assay, Humans, Immunoglobulin A/analysis, Immunoglobulin G/analysis, Immunoglobulin M/analysis, Macromolecular Substances, Rheumatoid Factor/analysis, Tetanus Toxoid/immunology, Yersinia enterocolitica/immunology
in
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
volume
98
issue
1-6
pages
249 - 254
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:0025233968
  • pmid:1690557
ISSN
0903-4641
DOI
10.1111/j.1699-0463.1990.tb01029.x
language
English
LU publication?
yes
id
6fbf7cd8-995a-4297-92aa-08829042687b
date added to LUP
2021-10-27 13:55:46
date last changed
2024-01-12 02:58:18
@article{6fbf7cd8-995a-4297-92aa-08829042687b,
  abstract     = {{<p>Enzyme-linked immunosorbent assays (ELISA) were developed for direct measurement of protein HC-IgA complexes (HC-IgA) in serum with antibody specificity for rabbit IgG (rheumatoid factor (RF) activity), lipopolysaccharide from Yersinia enterocolitica serotype O:3 (Y3) and tetanus toxoid (TT). About 80% of patients with rheumatoid arthritis had increased concentrations of HC-IgA-RF. The values were correlated with the concentrations of IgA-RF and IgM-RF. HC-IgA anti-Y3 was measured in 45 sera with anti-Y3 antibodies of IgM, IgG and IgA class. The HC-IgA anti-Y3 levels were correlated with those of anti-Y3 of IgG and IgM class, but not of IgA class. For HC-IgA anti-Y3, the closest correlation was that with the specific IgM antibody concentration, rs = 0.63 (p less than 0.001). In 25 normal sera, significant correlations were observed between HC-IgA anti-TT and specific antibodies of IgG and IgA class, but not of IgM class. In 107 sera containing IgA M-components, the total concentration of HC-IgA correlated poorly with both protein HC and with IgA concentrations. It was concluded that specific HC-IgA antibodies are normal constituents of serum, and that their concentrations are not directly related to the serum content of specific IgA antibodies.</p>}},
  author       = {{Truedsson, Lennart and Karypidis, Anna-Helena and Eberhardt, Kerstin and Grubb, Anders}},
  issn         = {{0903-4641}},
  keywords     = {{Alpha-Globulins/analysis; Enzyme-Linked Immunosorbent Assay; Humans; Immunoglobulin A/analysis; Immunoglobulin G/analysis; Immunoglobulin M/analysis; Macromolecular Substances; Rheumatoid Factor/analysis; Tetanus Toxoid/immunology; Yersinia enterocolitica/immunology}},
  language     = {{eng}},
  number       = {{1-6}},
  pages        = {{249--254}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{APMIS : acta pathologica, microbiologica, et immunologica Scandinavica}},
  title        = {{HC-IgA antibodies of different specificities are normally present in serum : quantitation by ELISA and relationship to the major Ig classes}},
  url          = {{http://dx.doi.org/10.1111/j.1699-0463.1990.tb01029.x}},
  doi          = {{10.1111/j.1699-0463.1990.tb01029.x}},
  volume       = {{98}},
  year         = {{1990}},
}