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In Depth Microscopy

Delaire, Tom LU and Galland, Rémi (2025) p.113-144
Abstract

This chapter provides an overview of the different optical sectioning methods that enable performing three dimensional (3D) imaging of possibly complex samples. It discusses their benefits and limitations regarding their penetration depth, their imaging speed, their photo-toxic effect or their complexity. Focusing on fluorescence imaging, the chapter dives into approaches that make it possible to provide an in-depth optical sectioning through spatial filtering or excitation light confinement, followed by alternative solutions based on image post-processing. The chapter also discusses actual challenges that are currently being adressed to continue the quest of imaging always more complex and physiologically relevant 3D living samples.... (More)

This chapter provides an overview of the different optical sectioning methods that enable performing three dimensional (3D) imaging of possibly complex samples. It discusses their benefits and limitations regarding their penetration depth, their imaging speed, their photo-toxic effect or their complexity. Focusing on fluorescence imaging, the chapter dives into approaches that make it possible to provide an in-depth optical sectioning through spatial filtering or excitation light confinement, followed by alternative solutions based on image post-processing. The chapter also discusses actual challenges that are currently being adressed to continue the quest of imaging always more complex and physiologically relevant 3D living samples. Confocal laser scanning microscopy (CLSM) is the most widely used 3D imaging technique due to its impressive optical sectioning capacity and ease of use. Spinning disk confocal microscopy provides an alternative method to increase CLSM speed, up to video rate, while largely preserving its optical sectioning capacity.

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Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
3D imaging technique, Confocal laser scanning microscopy, Fluorescence imaging, In-depth optical sectioning, Spinning disk confocal microscopy
host publication
Photonic Imaging for Biology : From Conventional Microscopy to Super-Resolution - From Conventional Microscopy to Super-Resolution
pages
32 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:105025090379
ISBN
9781394417896
9781789452228
DOI
10.1002/9781394417896.ch6
language
English
LU publication?
yes
additional info
Publisher Copyright: © ISTE Ltd 2025. All rights reserved.
id
6fe849b7-f8ea-4a3a-a3eb-b03077e7d73d
date added to LUP
2026-02-16 15:14:56
date last changed
2026-02-16 15:15:40
@inbook{6fe849b7-f8ea-4a3a-a3eb-b03077e7d73d,
  abstract     = {{<p>This chapter provides an overview of the different optical sectioning methods that enable performing three dimensional (3D) imaging of possibly complex samples. It discusses their benefits and limitations regarding their penetration depth, their imaging speed, their photo-toxic effect or their complexity. Focusing on fluorescence imaging, the chapter dives into approaches that make it possible to provide an in-depth optical sectioning through spatial filtering or excitation light confinement, followed by alternative solutions based on image post-processing. The chapter also discusses actual challenges that are currently being adressed to continue the quest of imaging always more complex and physiologically relevant 3D living samples. Confocal laser scanning microscopy (CLSM) is the most widely used 3D imaging technique due to its impressive optical sectioning capacity and ease of use. Spinning disk confocal microscopy provides an alternative method to increase CLSM speed, up to video rate, while largely preserving its optical sectioning capacity.</p>}},
  author       = {{Delaire, Tom and Galland, Rémi}},
  booktitle    = {{Photonic Imaging for Biology : From Conventional Microscopy to Super-Resolution}},
  isbn         = {{9781394417896}},
  keywords     = {{3D imaging technique; Confocal laser scanning microscopy; Fluorescence imaging; In-depth optical sectioning; Spinning disk confocal microscopy}},
  language     = {{eng}},
  month        = {{11}},
  pages        = {{113--144}},
  publisher    = {{John Wiley & Sons Inc.}},
  title        = {{In Depth Microscopy}},
  url          = {{http://dx.doi.org/10.1002/9781394417896.ch6}},
  doi          = {{10.1002/9781394417896.ch6}},
  year         = {{2025}},
}