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Carcinogenic polycyclic aromatic hydrocarbons induce CYP1A1 in human cells via a p53-dependent mechanism

Wohak, Laura E ; Krais, Annette M LU orcid ; Kucab, Jill E ; Stertmann, Julia ; Øvrebø, Steinar ; Seidel, Albrecht ; Phillips, David H. and Arlt, Volker M (2016) In Archives of Toxicology 90(2). p.291-304
Abstract

The tumour suppressor gene TP53 is mutated in more than 50 % of human tumours, making it one of the most important cancer genes. We have investigated the role of TP53 in cytochrome P450 (CYP)-mediated metabolic activation of three polycyclic aromatic hydrocarbons (PAHs) in a panel of isogenic colorectal HCT116 cells with differing TP53 status. Cells that were TP53(+/+), TP53(+/-), TP53(-/-), TP53(R248W/+) or TP53(R248W/-) were treated with benzo[a]pyrene (BaP), dibenz[a,h]anthracene and dibenzo[a,l]pyrene, and the formation of DNA adducts was measured by (32)P-postlabelling analysis. Each PAH formed significantly higher DNA adduct levels in TP53(+/+) cells than in the other cell lines. There were also significantly lower levels of PAH... (More)

The tumour suppressor gene TP53 is mutated in more than 50 % of human tumours, making it one of the most important cancer genes. We have investigated the role of TP53 in cytochrome P450 (CYP)-mediated metabolic activation of three polycyclic aromatic hydrocarbons (PAHs) in a panel of isogenic colorectal HCT116 cells with differing TP53 status. Cells that were TP53(+/+), TP53(+/-), TP53(-/-), TP53(R248W/+) or TP53(R248W/-) were treated with benzo[a]pyrene (BaP), dibenz[a,h]anthracene and dibenzo[a,l]pyrene, and the formation of DNA adducts was measured by (32)P-postlabelling analysis. Each PAH formed significantly higher DNA adduct levels in TP53(+/+) cells than in the other cell lines. There were also significantly lower levels of PAH metabolites in the culture media of these other cell lines. Bypass of the need for metabolic activation by treating cells with the corresponding reactive PAH-diol-epoxide metabolites resulted in similar adduct levels in all cell lines, which confirms that the influence of p53 is on the metabolism of the parent PAHs. Western blotting showed that CYP1A1 protein expression was induced to much greater extent in TP53(+/+) cells than in the other cell lines. CYP1A1 is inducible via the aryl hydrocarbon receptor (AHR), but we did not find that expression of AHR was dependent on p53; rather, we found that BaP-induced CYP1A1 expression was regulated through p53 binding to a p53 response element in the CYP1A1 promoter region, thereby enhancing its transcription. This study demonstrates a new pathway for CYP1A1 induction by environmental PAHs and reveals an emerging role for p53 in xenobiotic metabolism.

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publishing date
type
Contribution to journal
publication status
published
keywords
Basic Helix-Loop-Helix Transcription Factors, Benzo(a)pyrene, Carcinogens, Cell Survival, Cytochrome P-450 CYP1A1, Cytochrome P-450 CYP1B1, Cytochrome P-450 Enzyme Inducers, DNA Adducts, DNA Damage, HCT116 Cells, Humans, Inactivation, Metabolic, NAD(P)H Dehydrogenase (Quinone), Polycyclic Aromatic Hydrocarbons, Receptors, Aryl Hydrocarbon, Toxicity Tests, Tumor Suppressor Protein p53, Journal Article, Research Support, Non-U.S. Gov't
in
Archives of Toxicology
volume
90
issue
2
pages
14 pages
publisher
Springer
external identifiers
  • scopus:84958124444
  • pmid:25398514
ISSN
0340-5761
DOI
10.1007/s00204-014-1409-1
language
English
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no
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705d38d5-1d72-4f56-8458-32cebf89195f
date added to LUP
2017-10-17 15:29:11
date last changed
2024-09-17 10:09:10
@article{705d38d5-1d72-4f56-8458-32cebf89195f,
  abstract     = {{<p>The tumour suppressor gene TP53 is mutated in more than 50 % of human tumours, making it one of the most important cancer genes. We have investigated the role of TP53 in cytochrome P450 (CYP)-mediated metabolic activation of three polycyclic aromatic hydrocarbons (PAHs) in a panel of isogenic colorectal HCT116 cells with differing TP53 status. Cells that were TP53(+/+), TP53(+/-), TP53(-/-), TP53(R248W/+) or TP53(R248W/-) were treated with benzo[a]pyrene (BaP), dibenz[a,h]anthracene and dibenzo[a,l]pyrene, and the formation of DNA adducts was measured by (32)P-postlabelling analysis. Each PAH formed significantly higher DNA adduct levels in TP53(+/+) cells than in the other cell lines. There were also significantly lower levels of PAH metabolites in the culture media of these other cell lines. Bypass of the need for metabolic activation by treating cells with the corresponding reactive PAH-diol-epoxide metabolites resulted in similar adduct levels in all cell lines, which confirms that the influence of p53 is on the metabolism of the parent PAHs. Western blotting showed that CYP1A1 protein expression was induced to much greater extent in TP53(+/+) cells than in the other cell lines. CYP1A1 is inducible via the aryl hydrocarbon receptor (AHR), but we did not find that expression of AHR was dependent on p53; rather, we found that BaP-induced CYP1A1 expression was regulated through p53 binding to a p53 response element in the CYP1A1 promoter region, thereby enhancing its transcription. This study demonstrates a new pathway for CYP1A1 induction by environmental PAHs and reveals an emerging role for p53 in xenobiotic metabolism.</p>}},
  author       = {{Wohak, Laura E and Krais, Annette M and Kucab, Jill E and Stertmann, Julia and Øvrebø, Steinar and Seidel, Albrecht and Phillips, David H. and Arlt, Volker M}},
  issn         = {{0340-5761}},
  keywords     = {{Basic Helix-Loop-Helix Transcription Factors; Benzo(a)pyrene; Carcinogens; Cell Survival; Cytochrome P-450 CYP1A1; Cytochrome P-450 CYP1B1; Cytochrome P-450 Enzyme Inducers; DNA Adducts; DNA Damage; HCT116 Cells; Humans; Inactivation, Metabolic; NAD(P)H Dehydrogenase (Quinone); Polycyclic Aromatic Hydrocarbons; Receptors, Aryl Hydrocarbon; Toxicity Tests; Tumor Suppressor Protein p53; Journal Article; Research Support, Non-U.S. Gov't}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{291--304}},
  publisher    = {{Springer}},
  series       = {{Archives of Toxicology}},
  title        = {{Carcinogenic polycyclic aromatic hydrocarbons induce CYP1A1 in human cells via a p53-dependent mechanism}},
  url          = {{http://dx.doi.org/10.1007/s00204-014-1409-1}},
  doi          = {{10.1007/s00204-014-1409-1}},
  volume       = {{90}},
  year         = {{2016}},
}