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Design, viualisation and utilisation of enzyme microstructures built on solid surfaces

Gaspar, Szilveszter LU ; Schumann, W ; Laurell, Thomas LU and Csöregi, Elisabeth LU (2002) In Reviews in Analytical Chemistry 21(4). p.245-266
Abstract
Abstract in Undetermined
Immobilized bioelements offer additional sensitivity and/or selectivity in analytical techniques, in biotechnological devices, and in different medical applications. Great research interest has recently been focused in all these fields on miniaturization techniques, special attention being accorded to the patterning of biomolecules (e.g. enzymes) on solid surfaces with micrometer resolution. Novel microanalysis systems consider the use of enzyme microstructures as e.g. part of mu- Total Analysis System platforms as biochemical microreactors or detection units. This mini-review highlights recent advances in the creation of enzyme microstructures by indirect methods (photolithography, microcontact printing. etc.)... (More)
Abstract in Undetermined
Immobilized bioelements offer additional sensitivity and/or selectivity in analytical techniques, in biotechnological devices, and in different medical applications. Great research interest has recently been focused in all these fields on miniaturization techniques, special attention being accorded to the patterning of biomolecules (e.g. enzymes) on solid surfaces with micrometer resolution. Novel microanalysis systems consider the use of enzyme microstructures as e.g. part of mu- Total Analysis System platforms as biochemical microreactors or detection units. This mini-review highlights recent advances in the creation of enzyme microstructures by indirect methods (photolithography, microcontact printing. etc.) and active placement methods (electrospraying, microdispensing, etc.). Some key visualization techniques of enzyme microstructures (fluorescence microscopy, scanning electrochemical microscopy, etc.) are also mentioned together with examples of their applications or application possibilities. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
scanning electrochemical microscopy, enzyme microstructures, microdispenser
in
Reviews in Analytical Chemistry
volume
21
issue
4
pages
245 - 266
publisher
Freund Publishing House Ltd
external identifiers
  • wos:000182163400001
  • scopus:0036973144
ISSN
0048-752X
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biomedical Engineering (011200011), Analytical Chemistry (S/LTH) (011001004)
id
c363d367-baf9-4e8e-be4f-249dc54d0986 (old id 710174)
date added to LUP
2016-04-04 09:40:18
date last changed
2022-01-29 18:59:37
@article{c363d367-baf9-4e8e-be4f-249dc54d0986,
  abstract     = {{Abstract in Undetermined<br/>Immobilized bioelements offer additional sensitivity and/or selectivity in analytical techniques, in biotechnological devices, and in different medical applications. Great research interest has recently been focused in all these fields on miniaturization techniques, special attention being accorded to the patterning of biomolecules (e.g. enzymes) on solid surfaces with micrometer resolution. Novel microanalysis systems consider the use of enzyme microstructures as e.g. part of mu- Total Analysis System platforms as biochemical microreactors or detection units. This mini-review highlights recent advances in the creation of enzyme microstructures by indirect methods (photolithography, microcontact printing. etc.) and active placement methods (electrospraying, microdispensing, etc.). Some key visualization techniques of enzyme microstructures (fluorescence microscopy, scanning electrochemical microscopy, etc.) are also mentioned together with examples of their applications or application possibilities.}},
  author       = {{Gaspar, Szilveszter and Schumann, W and Laurell, Thomas and Csöregi, Elisabeth}},
  issn         = {{0048-752X}},
  keywords     = {{scanning electrochemical microscopy; enzyme microstructures; microdispenser}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{245--266}},
  publisher    = {{Freund Publishing House Ltd}},
  series       = {{Reviews in Analytical Chemistry}},
  title        = {{Design, viualisation and utilisation of enzyme microstructures built on solid surfaces}},
  volume       = {{21}},
  year         = {{2002}},
}