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Isolation and characterization of a stress responsive small GTP-binding protein AhRabG3b in peanut (Arachis hypogaea L.)

Sui, Jiong-Ming ; Li, Rui ; Fan, Qian-Cheng ; Song, Lin LU ; Zheng, Chun-Hua ; Wang, Jing-Shan ; Qiao, Li-Xian and Yu, Shan-Lin (2013) In Euphytica 189(2). p.161-172
Abstract
Rab7 is a member of small GTP-binding protein family involved in intracellular vesicle trafficking from late endosome to vacuole, some of them are found to be responsive by different environmental stresses. Here we report the isolation of a Rab7 (RabG3b) cDNA from peanut (Arachis hypogaea). Overexpression of the open reading frame (ORF) of Rab7 cDNA encoding 205 amino acids in E. coli conferred the cells tolerance to various abiotic stresses. Transcription of AhRabG3b was differentially up-regulated by different environmental stimuli such as cold, dehydration, NaCl and the plant hormone ABA. Overexpression of the AhRabG3b exhibited enhanced tolerance to drought and salinity stresses in transgenic peanut. These results suggested that the... (More)
Rab7 is a member of small GTP-binding protein family involved in intracellular vesicle trafficking from late endosome to vacuole, some of them are found to be responsive by different environmental stresses. Here we report the isolation of a Rab7 (RabG3b) cDNA from peanut (Arachis hypogaea). Overexpression of the open reading frame (ORF) of Rab7 cDNA encoding 205 amino acids in E. coli conferred the cells tolerance to various abiotic stresses. Transcription of AhRabG3b was differentially up-regulated by different environmental stimuli such as cold, dehydration, NaCl and the plant hormone ABA. Overexpression of the AhRabG3b exhibited enhanced tolerance to drought and salinity stresses in transgenic peanut. These results suggested that the AhRabG3b be a potential candidate gene responsible for both salinity and dehydration tolerance in cultivated peanut. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Peanut (Arachis hypogaea L.), AhRabG3b, Abiotic stress, Overexpression
in
Euphytica
volume
189
issue
2
pages
161 - 172
publisher
Springer
external identifiers
  • wos:000312191200001
  • scopus:84870735459
ISSN
0014-2336
DOI
10.1007/s10681-012-0739-4
language
English
LU publication?
yes
id
7152c994-f00e-4fbc-bb74-666303c63065 (old id 3658154)
date added to LUP
2016-04-01 13:41:10
date last changed
2022-04-06 06:22:26
@article{7152c994-f00e-4fbc-bb74-666303c63065,
  abstract     = {{Rab7 is a member of small GTP-binding protein family involved in intracellular vesicle trafficking from late endosome to vacuole, some of them are found to be responsive by different environmental stresses. Here we report the isolation of a Rab7 (RabG3b) cDNA from peanut (Arachis hypogaea). Overexpression of the open reading frame (ORF) of Rab7 cDNA encoding 205 amino acids in E. coli conferred the cells tolerance to various abiotic stresses. Transcription of AhRabG3b was differentially up-regulated by different environmental stimuli such as cold, dehydration, NaCl and the plant hormone ABA. Overexpression of the AhRabG3b exhibited enhanced tolerance to drought and salinity stresses in transgenic peanut. These results suggested that the AhRabG3b be a potential candidate gene responsible for both salinity and dehydration tolerance in cultivated peanut.}},
  author       = {{Sui, Jiong-Ming and Li, Rui and Fan, Qian-Cheng and Song, Lin and Zheng, Chun-Hua and Wang, Jing-Shan and Qiao, Li-Xian and Yu, Shan-Lin}},
  issn         = {{0014-2336}},
  keywords     = {{Peanut (Arachis hypogaea L.); AhRabG3b; Abiotic stress; Overexpression}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{161--172}},
  publisher    = {{Springer}},
  series       = {{Euphytica}},
  title        = {{Isolation and characterization of a stress responsive small GTP-binding protein AhRabG3b in peanut (Arachis hypogaea L.)}},
  url          = {{http://dx.doi.org/10.1007/s10681-012-0739-4}},
  doi          = {{10.1007/s10681-012-0739-4}},
  volume       = {{189}},
  year         = {{2013}},
}