Exprese MMP-26 v endometrialnich explantech pod vlivem estradiolu a progesteronu
(2004) In Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne 69(6). p.467-471- Abstract
- OBJECTIVE: To examine possible estrogen dependent endometrial expression of MMP-26 in vitro. DESIGN: Experimental study. SETTING: Department of Obstetrics and Gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obstetrics and Gynecology, University Hospital, Lund, Sweden. METHODS: We studied MMP-26 mRNA in 14 normal endometrial samples obtained from the proliferative phase of the menstrual cycle. Samples were cultured for five days either with estradiol alone or in combination with progesterone. Samples cultured with ethanol represented control groups. MMP-26 mRNA expression was examined in frozen samples using in situ hybridization. Immunohistochemistry was used to study the... (More)
- OBJECTIVE: To examine possible estrogen dependent endometrial expression of MMP-26 in vitro. DESIGN: Experimental study. SETTING: Department of Obstetrics and Gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obstetrics and Gynecology, University Hospital, Lund, Sweden. METHODS: We studied MMP-26 mRNA in 14 normal endometrial samples obtained from the proliferative phase of the menstrual cycle. Samples were cultured for five days either with estradiol alone or in combination with progesterone. Samples cultured with ethanol represented control groups. MMP-26 mRNA expression was examined in frozen samples using in situ hybridization. Immunohistochemistry was used to study the presence of estrogen and progesterone receptors in endometrial explants. RESULTS: MMP-26 mRNA expression was highest in fresh (non cultured) samples. Signal intensity decreased during the first two days of culture and was negligable in the following days. Nuclear intensity for estrogen and progesterone receptor was high after five days of culture. CONCLUSION: We did not find MMP-26 mRNA in vitro expression to be directly estrogen dependent. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1129633
- author
- Pilka, Radovan LU ; Kudela, M ; Hansson, Stefan LU and Casslén, Bertil LU
- organization
- alternative title
- MMP-26 expression in endometrial explants treated with estradiol and progesterone
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne
- volume
- 69
- issue
- 6
- pages
- 467 - 471
- publisher
- Ceska Lekarska Spolecnost J. E. Purkyne
- external identifiers
-
- pmid:15633416
- scopus:9644255836
- ISSN
- 1210-7832
- language
- Czech
- LU publication?
- yes
- id
- 71a86fd8-083d-41b0-98c5-44f23e660d7b (old id 1129633)
- date added to LUP
- 2016-04-01 15:25:26
- date last changed
- 2022-06-20 13:29:22
@article{71a86fd8-083d-41b0-98c5-44f23e660d7b, abstract = {{OBJECTIVE: To examine possible estrogen dependent endometrial expression of MMP-26 in vitro. DESIGN: Experimental study. SETTING: Department of Obstetrics and Gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obstetrics and Gynecology, University Hospital, Lund, Sweden. METHODS: We studied MMP-26 mRNA in 14 normal endometrial samples obtained from the proliferative phase of the menstrual cycle. Samples were cultured for five days either with estradiol alone or in combination with progesterone. Samples cultured with ethanol represented control groups. MMP-26 mRNA expression was examined in frozen samples using in situ hybridization. Immunohistochemistry was used to study the presence of estrogen and progesterone receptors in endometrial explants. RESULTS: MMP-26 mRNA expression was highest in fresh (non cultured) samples. Signal intensity decreased during the first two days of culture and was negligable in the following days. Nuclear intensity for estrogen and progesterone receptor was high after five days of culture. CONCLUSION: We did not find MMP-26 mRNA in vitro expression to be directly estrogen dependent.}}, author = {{Pilka, Radovan and Kudela, M and Hansson, Stefan and Casslén, Bertil}}, issn = {{1210-7832}}, language = {{cze}}, number = {{6}}, pages = {{467--471}}, publisher = {{Ceska Lekarska Spolecnost J. E. Purkyne}}, series = {{Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne}}, title = {{Exprese MMP-26 v endometrialnich explantech pod vlivem estradiolu a progesteronu}}, volume = {{69}}, year = {{2004}}, }