Evaluation of the production of exopolysaccharides by two strains of the thermophilic bacterium Rhodothermus marinus
(2017) In Carbohydrate Polymers 156. p.1-8- Abstract
The thermophile Rhodothermus marinus produces extracellular polysaccharides (EPSs) that forms a distinct cellular capsule. Here, the first data on EPS production in strains DSM4252(T) and MAT493 are reported and compared. Cultures of both strains, supplemented with either glucose, sucrose, lactose or maltose showed that the EPS were produced both in the exponential and stationary growth phase and that production in the exponential phase was boosted by maltose supplementation, while stationary phase production was boosted by lactose. The latter was higher, resulting in 8.8 (DSM4252(T)) and 13.7mg EPS/g cell dry weight (MAT493) in cultures in marine broth supplemented with 10g/L lactose. The EPSs were heteropolymeric with an average... (More)
The thermophile Rhodothermus marinus produces extracellular polysaccharides (EPSs) that forms a distinct cellular capsule. Here, the first data on EPS production in strains DSM4252(T) and MAT493 are reported and compared. Cultures of both strains, supplemented with either glucose, sucrose, lactose or maltose showed that the EPS were produced both in the exponential and stationary growth phase and that production in the exponential phase was boosted by maltose supplementation, while stationary phase production was boosted by lactose. The latter was higher, resulting in 8.8 (DSM4252(T)) and 13.7mg EPS/g cell dry weight (MAT493) in cultures in marine broth supplemented with 10g/L lactose. The EPSs were heteropolymeric with an average molecular weight of 8×10(4)Da and different monosaccharides, including arabinose and xylose. FT-IR spectroscopy revealed presence of hydroxyl, carboxyl, N-acetyl, amine, and sulfate ester groups, showing that R. marinus produces unusual sulfated EPS with high arabinose and xylose content.
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- author
- Sardari, Roya R R LU ; Kulcinskaja, Evelina LU ; Ron, Emanuel Y C LU ; Björnsdóttir, Snædís ; Friðjónsson, Ólafur H. ; Hreggviðsson, Guðmundur Óli and Karlsson, Eva Nordberg LU
- organization
- publishing date
- 2017-01-20
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- EPS, Exopolysaccharide, Heteropolymer, Rhodothermus marinus
- in
- Carbohydrate Polymers
- volume
- 156
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:27842803
- wos:000388110900001
- scopus:84985960370
- ISSN
- 0144-8617
- DOI
- 10.1016/j.carbpol.2016.08.062
- language
- English
- LU publication?
- yes
- id
- 71ae6ac1-39a0-4259-8d20-f764fa5cec57
- date added to LUP
- 2016-09-20 14:43:30
- date last changed
- 2024-10-06 02:19:34
@article{71ae6ac1-39a0-4259-8d20-f764fa5cec57, abstract = {{<p>The thermophile Rhodothermus marinus produces extracellular polysaccharides (EPSs) that forms a distinct cellular capsule. Here, the first data on EPS production in strains DSM4252(T) and MAT493 are reported and compared. Cultures of both strains, supplemented with either glucose, sucrose, lactose or maltose showed that the EPS were produced both in the exponential and stationary growth phase and that production in the exponential phase was boosted by maltose supplementation, while stationary phase production was boosted by lactose. The latter was higher, resulting in 8.8 (DSM4252(T)) and 13.7mg EPS/g cell dry weight (MAT493) in cultures in marine broth supplemented with 10g/L lactose. The EPSs were heteropolymeric with an average molecular weight of 8×10(4)Da and different monosaccharides, including arabinose and xylose. FT-IR spectroscopy revealed presence of hydroxyl, carboxyl, N-acetyl, amine, and sulfate ester groups, showing that R. marinus produces unusual sulfated EPS with high arabinose and xylose content.</p>}}, author = {{Sardari, Roya R R and Kulcinskaja, Evelina and Ron, Emanuel Y C and Björnsdóttir, Snædís and Friðjónsson, Ólafur H. and Hreggviðsson, Guðmundur Óli and Karlsson, Eva Nordberg}}, issn = {{0144-8617}}, keywords = {{EPS; Exopolysaccharide; Heteropolymer; Rhodothermus marinus}}, language = {{eng}}, month = {{01}}, pages = {{1--8}}, publisher = {{Elsevier}}, series = {{Carbohydrate Polymers}}, title = {{Evaluation of the production of exopolysaccharides by two strains of the thermophilic bacterium Rhodothermus marinus}}, url = {{http://dx.doi.org/10.1016/j.carbpol.2016.08.062}}, doi = {{10.1016/j.carbpol.2016.08.062}}, volume = {{156}}, year = {{2017}}, }