A murine pressure ulcer model for evaluating persistence and treatment of Staphylococcus aureus infection
Tavecchio, Michele LU ; Fanni, Silvia LU ; Wu, Xuemin LU ; Petruk, Ganna LU
; Puthia, Manoj
LU
and Schmidtchen, Artur
LU
(2025)
In Frontiers in Medicine
12.
p.01-11
- Abstract
Chronic wounds, particularly pressure ulcers, pose significant healthcare challenges, especially in the elderly population. This study presents an experimental murine model of chronically infected pressure ulcers using a single cycle of magnet-induced ischemic injury combined with infection by bioluminescent Staphylococcus aureus. The model addresses previous limitations in studying pressure ulcer infection pathogenesis and evaluating treatment efficacy. By combining this model with in vivo imaging system (IVIS) technology, we achieved real-time, non-invasive monitoring of infection dynamics. This approach demonstrated persistent pressure ulcer wound infection and provided temporal and spatial data on infection status. To validate the... (More)
Chronic wounds, particularly pressure ulcers, pose significant healthcare challenges, especially in the elderly population. This study presents an experimental murine model of chronically infected pressure ulcers using a single cycle of magnet-induced ischemic injury combined with infection by bioluminescent Staphylococcus aureus. The model addresses previous limitations in studying pressure ulcer infection pathogenesis and evaluating treatment efficacy. By combining this model with in vivo imaging system (IVIS) technology, we achieved real-time, non-invasive monitoring of infection dynamics. This approach demonstrated persistent pressure ulcer wound infection and provided temporal and spatial data on infection status. To validate the model’s utility, we evaluated the antimicrobial efficacy of TCP-25, a synthetic host defense peptide, delivered in a topical gel formulation. Our findings highlight the potential of this model for investigating wound infection mechanisms, bacterial persistence, and therapeutic interventions. This innovative approach represents a significant advancement in pressure ulcer research, offering new opportunities for developing effective treatment strategies and improving patient outcomes.
(Less)
- author
- Tavecchio, Michele
LU
; Fanni, Silvia
LU
; Wu, Xuemin
LU
; Petruk, Ganna
LU
; Puthia, Manoj
LU
and Schmidtchen, Artur
LU
- organization
- publishing date
- 2025
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- bioimaging, pressure ulcer, Staphylococcus aureus, TCP-25, wound infection
- in
- Frontiers in Medicine
- volume
- 12
- article number
- 1561732
- pages
- 01 - 11
- publisher
- Frontiers Media S. A.
- external identifiers
-
- scopus:105002639227
- pmid:40248082
- ISSN
- 2296-858X
- DOI
- 10.3389/fmed.2025.1561732
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: Copyright © 2025 Tavecchio, Fanni, Wu, Petruk, Puthia and Schmidtchen.
- id
- 722db100-2738-4132-9af3-6cfdd9fb7499
- date added to LUP
- 2025-04-22 15:06:03
- date last changed
- 2025-06-03 18:39:53
@article{722db100-2738-4132-9af3-6cfdd9fb7499,
abstract = {{<p>Chronic wounds, particularly pressure ulcers, pose significant healthcare challenges, especially in the elderly population. This study presents an experimental murine model of chronically infected pressure ulcers using a single cycle of magnet-induced ischemic injury combined with infection by bioluminescent Staphylococcus aureus. The model addresses previous limitations in studying pressure ulcer infection pathogenesis and evaluating treatment efficacy. By combining this model with in vivo imaging system (IVIS) technology, we achieved real-time, non-invasive monitoring of infection dynamics. This approach demonstrated persistent pressure ulcer wound infection and provided temporal and spatial data on infection status. To validate the model’s utility, we evaluated the antimicrobial efficacy of TCP-25, a synthetic host defense peptide, delivered in a topical gel formulation. Our findings highlight the potential of this model for investigating wound infection mechanisms, bacterial persistence, and therapeutic interventions. This innovative approach represents a significant advancement in pressure ulcer research, offering new opportunities for developing effective treatment strategies and improving patient outcomes.</p>}},
author = {{Tavecchio, Michele and Fanni, Silvia and Wu, Xuemin and Petruk, Ganna and Puthia, Manoj and Schmidtchen, Artur}},
issn = {{2296-858X}},
keywords = {{bioimaging; pressure ulcer; Staphylococcus aureus; TCP-25; wound infection}},
language = {{eng}},
pages = {{01--11}},
publisher = {{Frontiers Media S. A.}},
series = {{Frontiers in Medicine}},
title = {{A murine pressure ulcer model for evaluating persistence and treatment of Staphylococcus aureus infection}},
url = {{http://dx.doi.org/10.3389/fmed.2025.1561732}},
doi = {{10.3389/fmed.2025.1561732}},
volume = {{12}},
year = {{2025}},
}