Advanced

FACS binding assay for analysing GDNF interactions.

Quintino, Luis LU ; Baudet, Aurelie LU ; Larsson, Jonas LU and Lundberg, Cecilia LU (2013) In Journal of Neuroscience Methods 218(1). p.25-28
Abstract
Glial cell-line derived neurotrophic factor (GDNF) is a secreted protein with great therapeutic potential. However, in order to analyse the interactions between GDNF and its receptors, researchers have been mostly dependent of radioactive binding assays. We developed a FACS-based binding assay for GDNF as an alternative to current methods. We demonstrated that the FACS-based assay using TGW cells allowed readily detection of GDNF binding and displacement to endogenous receptors. The dissociation constant and half maximal inhibitory concentration obtained were comparable to other studies using standard binding assays. Overall, this FACS-based, simple to perform and adaptable to high throughput setup, provides a safer and reliable... (More)
Glial cell-line derived neurotrophic factor (GDNF) is a secreted protein with great therapeutic potential. However, in order to analyse the interactions between GDNF and its receptors, researchers have been mostly dependent of radioactive binding assays. We developed a FACS-based binding assay for GDNF as an alternative to current methods. We demonstrated that the FACS-based assay using TGW cells allowed readily detection of GDNF binding and displacement to endogenous receptors. The dissociation constant and half maximal inhibitory concentration obtained were comparable to other studies using standard binding assays. Overall, this FACS-based, simple to perform and adaptable to high throughput setup, provides a safer and reliable alternative to radioactive methods. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Neuroscience Methods
volume
218
issue
1
pages
25 - 28
publisher
Elsevier
external identifiers
  • wos:000322692900004
  • pmid:23669067
  • scopus:84878743571
  • pmid:23669067
ISSN
1872-678X
DOI
10.1016/j.jneumeth.2013.04.006
language
English
LU publication?
yes
id
7267bcc9-ef3f-494d-b8fe-34125ae72c0a (old id 3804530)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23669067?dopt=Abstract
date added to LUP
2016-04-01 09:52:40
date last changed
2020-01-05 03:28:12
@article{7267bcc9-ef3f-494d-b8fe-34125ae72c0a,
  abstract     = {Glial cell-line derived neurotrophic factor (GDNF) is a secreted protein with great therapeutic potential. However, in order to analyse the interactions between GDNF and its receptors, researchers have been mostly dependent of radioactive binding assays. We developed a FACS-based binding assay for GDNF as an alternative to current methods. We demonstrated that the FACS-based assay using TGW cells allowed readily detection of GDNF binding and displacement to endogenous receptors. The dissociation constant and half maximal inhibitory concentration obtained were comparable to other studies using standard binding assays. Overall, this FACS-based, simple to perform and adaptable to high throughput setup, provides a safer and reliable alternative to radioactive methods.},
  author       = {Quintino, Luis and Baudet, Aurelie and Larsson, Jonas and Lundberg, Cecilia},
  issn         = {1872-678X},
  language     = {eng},
  number       = {1},
  pages        = {25--28},
  publisher    = {Elsevier},
  series       = {Journal of Neuroscience Methods},
  title        = {FACS binding assay for analysing GDNF interactions.},
  url          = {http://dx.doi.org/10.1016/j.jneumeth.2013.04.006},
  doi          = {10.1016/j.jneumeth.2013.04.006},
  volume       = {218},
  year         = {2013},
}