Leukocyte adhesion in aorta and femoral artery in vivo is mediated by LFA-1
(2004) In Inflammation Research 53(10). p.523-527- Abstract
- Objective: Cytokine-induced recruitment of leukocytes is an early feature during arterial injury and atherosclerotic plaque formation. The aim of this study was to analyze the role of the beta(2)-integrin lymphocyte function-associated antigen-1 (LFA- 1; CD11a/CD18) in cytokine-triggered firm leukocyte adhesion to arterial endothelium in vivo. Material and Methods: Intravital fluorescence microscopy was used to study leukocyte firm adhesion in the mouse aorta and femoral artery in response to combined local challenge with TNF-alpha and IL-1beta. Results: In wild-type (WT) mice, cytokine stimulation resulted in firm adhesion of 14.6 +/- 2.8 and 11.3 +/- 1.3 leukocytes/mm along the endothelium in the aorta and femoral artery (P < 0.05 vs.... (More)
- Objective: Cytokine-induced recruitment of leukocytes is an early feature during arterial injury and atherosclerotic plaque formation. The aim of this study was to analyze the role of the beta(2)-integrin lymphocyte function-associated antigen-1 (LFA- 1; CD11a/CD18) in cytokine-triggered firm leukocyte adhesion to arterial endothelium in vivo. Material and Methods: Intravital fluorescence microscopy was used to study leukocyte firm adhesion in the mouse aorta and femoral artery in response to combined local challenge with TNF-alpha and IL-1beta. Results: In wild-type (WT) mice, cytokine stimulation resulted in firm adhesion of 14.6 +/- 2.8 and 11.3 +/- 1.3 leukocytes/mm along the endothelium in the aorta and femoral artery (P < 0.05 vs. PBS-treated controls, n = 5-6). Notably, the number of firmly adherent leukocytes in aorta and femoral artery of cytokine-stimulated LFA-1-deficient animals was reduced by 54% and 92% indicating an important role of LFA-1 in leukocyte adhesion to arterial endothelium (P < 0.05 vs. controls, n = 5-6). In addition, pretreatment of WT mice with a monoclonal antibody (mAb) directed against murine LFA-1 attenuated the leukocyte adhesive response by 60% and 86% in aorta and femoral artery, respectively (P < 0.05 vs. control mAb-treated WT, n = 5-12). Conclusion: These novel data demonstrate that cytokine-induced firm leukocyte adhesion in the mouse aorta and femoral artery is LFA-1-dependent in vivo, which may implicate an important role for this β(2)-integrin leukocyte extravasation. in arterial injury and atherogenesis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/262442
- author
- Schramm, R ; Menger, M D ; Schaefers, H J and Thorlacius, Henrik LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- leukocyte recruitment, beta(2)-integrins, inflammation, atherosclerosis, cytokines
- in
- Inflammation Research
- volume
- 53
- issue
- 10
- pages
- 523 - 527
- publisher
- Birkhäuser Verlag
- external identifiers
-
- wos:000224915700004
- pmid:15597146
- scopus:12144262055
- pmid:15597146
- ISSN
- 1420-908X
- DOI
- 10.1007/s00011-004-1285-x
- language
- English
- LU publication?
- yes
- id
- 7327e833-b02a-4284-8b24-5b38e822d822 (old id 262442)
- date added to LUP
- 2016-04-01 12:37:05
- date last changed
- 2022-01-27 07:33:54
@article{7327e833-b02a-4284-8b24-5b38e822d822,
abstract = {{Objective: Cytokine-induced recruitment of leukocytes is an early feature during arterial injury and atherosclerotic plaque formation. The aim of this study was to analyze the role of the beta(2)-integrin lymphocyte function-associated antigen-1 (LFA- 1; CD11a/CD18) in cytokine-triggered firm leukocyte adhesion to arterial endothelium in vivo. Material and Methods: Intravital fluorescence microscopy was used to study leukocyte firm adhesion in the mouse aorta and femoral artery in response to combined local challenge with TNF-alpha and IL-1beta. Results: In wild-type (WT) mice, cytokine stimulation resulted in firm adhesion of 14.6 +/- 2.8 and 11.3 +/- 1.3 leukocytes/mm along the endothelium in the aorta and femoral artery (P < 0.05 vs. PBS-treated controls, n = 5-6). Notably, the number of firmly adherent leukocytes in aorta and femoral artery of cytokine-stimulated LFA-1-deficient animals was reduced by 54% and 92% indicating an important role of LFA-1 in leukocyte adhesion to arterial endothelium (P < 0.05 vs. controls, n = 5-6). In addition, pretreatment of WT mice with a monoclonal antibody (mAb) directed against murine LFA-1 attenuated the leukocyte adhesive response by 60% and 86% in aorta and femoral artery, respectively (P < 0.05 vs. control mAb-treated WT, n = 5-12). Conclusion: These novel data demonstrate that cytokine-induced firm leukocyte adhesion in the mouse aorta and femoral artery is LFA-1-dependent in vivo, which may implicate an important role for this β(2)-integrin leukocyte extravasation. in arterial injury and atherogenesis.}},
author = {{Schramm, R and Menger, M D and Schaefers, H J and Thorlacius, Henrik}},
issn = {{1420-908X}},
keywords = {{leukocyte recruitment; beta(2)-integrins; inflammation; atherosclerosis; cytokines}},
language = {{eng}},
number = {{10}},
pages = {{523--527}},
publisher = {{Birkhäuser Verlag}},
series = {{Inflammation Research}},
title = {{Leukocyte adhesion in aorta and femoral artery in vivo is mediated by LFA-1}},
url = {{http://dx.doi.org/10.1007/s00011-004-1285-x}},
doi = {{10.1007/s00011-004-1285-x}},
volume = {{53}},
year = {{2004}},
}