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Large crystal growth for neutron protein crystallography

Budayova-Spano, Monika ; Koruza, Katarina LU and Fisher, Zoë LU (2020) In Methods in Enzymology
Abstract

The use of neutron protein crystallography (NPX) is expanding rapidly, with most structures determined in the last decade. This growth is stimulated by a number of developments, spanning from the building of new NPX beamlines to the availability of improved software for structure refinement. The main bottleneck preventing structural biologists from adding NPX to the suite of methods commonly used is the large volume of the individual crystals required for a successful experiment. A survey of deposited NPX structures in the Protein Data Bank shows that about two-thirds came from crystals prepared using vapor diffusion, while batch and dialysis-based methods all-together contribute to most of the remaining one-third. This chapter explains... (More)

The use of neutron protein crystallography (NPX) is expanding rapidly, with most structures determined in the last decade. This growth is stimulated by a number of developments, spanning from the building of new NPX beamlines to the availability of improved software for structure refinement. The main bottleneck preventing structural biologists from adding NPX to the suite of methods commonly used is the large volume of the individual crystals required for a successful experiment. A survey of deposited NPX structures in the Protein Data Bank shows that about two-thirds came from crystals prepared using vapor diffusion, while batch and dialysis-based methods all-together contribute to most of the remaining one-third. This chapter explains the underlying principles of these protein crystallization methods and provides practical examples that may help others to successfully prepare large crystals for NPX.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
epub
subject
keywords
Dialysis, Microseeding, Phase diagram, Vapor diffusion
host publication
Methods in Enzymology
series title
Methods in Enzymology
publisher
Academic Press
external identifiers
  • pmid:32093834
  • scopus:85078158714
ISSN
1557-7988
0076-6879
DOI
10.1016/bs.mie.2019.11.015
language
English
LU publication?
yes
id
73ce3e19-bbb1-4cab-b473-66c831ececc3
date added to LUP
2020-02-10 12:45:03
date last changed
2020-07-08 05:12:16
@inbook{73ce3e19-bbb1-4cab-b473-66c831ececc3,
  abstract     = {<p>The use of neutron protein crystallography (NPX) is expanding rapidly, with most structures determined in the last decade. This growth is stimulated by a number of developments, spanning from the building of new NPX beamlines to the availability of improved software for structure refinement. The main bottleneck preventing structural biologists from adding NPX to the suite of methods commonly used is the large volume of the individual crystals required for a successful experiment. A survey of deposited NPX structures in the Protein Data Bank shows that about two-thirds came from crystals prepared using vapor diffusion, while batch and dialysis-based methods all-together contribute to most of the remaining one-third. This chapter explains the underlying principles of these protein crystallization methods and provides practical examples that may help others to successfully prepare large crystals for NPX.</p>},
  author       = {Budayova-Spano, Monika and Koruza, Katarina and Fisher, Zoë},
  booktitle    = {Methods in Enzymology},
  issn         = {1557-7988},
  language     = {eng},
  month        = {01},
  publisher    = {Academic Press},
  series       = {Methods in Enzymology},
  title        = {Large crystal growth for neutron protein crystallography},
  url          = {http://dx.doi.org/10.1016/bs.mie.2019.11.015},
  doi          = {10.1016/bs.mie.2019.11.015},
  year         = {2020},
}