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Implementation of Molecularly Imprinted Polymer Beads for Surface Enhanced Raman Detection

Kamra, Tripta LU ; Zhou, Tongchang LU ; Montelius, Lars LU ; Schnadt, Joachim LU and Ye, Lei LU (2015) In Analytical Chemistry 87(10). p.5056-5061
Abstract
Molecularly imprinted polymers (MIPs) have a predesigned molecular recognition capability that can be used to build robust chemical sensors. MIP-based chemical sensors allow label-free detection and are particularly interesting due to their simple operation. In this work we report the use of thiol-terminated MIP microspheres to construct surfaces for detection of a model organic analyte, nicotine, by surface enhanced Raman scattering (SERS). The nicotine-imprinted microspheres are synthesized by RAFT precipitation polymerization and converted into thiol-terminated microspheres through aminolysis. The thiol groups on the MIP surface allow the microspheres to be immobilized on a gold-coated substrate. Three different strategies are... (More)
Molecularly imprinted polymers (MIPs) have a predesigned molecular recognition capability that can be used to build robust chemical sensors. MIP-based chemical sensors allow label-free detection and are particularly interesting due to their simple operation. In this work we report the use of thiol-terminated MIP microspheres to construct surfaces for detection of a model organic analyte, nicotine, by surface enhanced Raman scattering (SERS). The nicotine-imprinted microspheres are synthesized by RAFT precipitation polymerization and converted into thiol-terminated microspheres through aminolysis. The thiol groups on the MIP surface allow the microspheres to be immobilized on a gold-coated substrate. Three different strategies are investigated to achieve surface enhanced Raman scattering in the vicinity of the imprinted sites: (1) direct sputtering of gold nanoparticles, (2) immobilization of gold colloids through the MIPs thiol groups, and (3) trapping of the MIP microspheres in a patterned SERS substrate. For the first time we show that large MIP microspheres can be turned into selective SERS surfaces through the three different approaches of assembly. The MIP-based sensing surfaces are used to detect nicotine to demonstrate the proof of concept. As synthesis and surface functionalization of MIP microspheres and nanoparticles are well established, the methods reported in this work are handy and efficient for constructing label-free chemical sensors, in particular for those based on SERS detection. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
87
issue
10
pages
5056 - 5061
publisher
The American Chemical Society
external identifiers
  • wos:000355057700008
  • scopus:84929629326
ISSN
1520-6882
DOI
10.1021/acs.analchem.5b00774
language
English
LU publication?
yes
id
d6483e28-72a7-4c0d-a446-ce0fe6f95dbd (old id 7410677)
date added to LUP
2015-06-29 11:24:27
date last changed
2017-10-22 03:26:46
@article{d6483e28-72a7-4c0d-a446-ce0fe6f95dbd,
  abstract     = {Molecularly imprinted polymers (MIPs) have a predesigned molecular recognition capability that can be used to build robust chemical sensors. MIP-based chemical sensors allow label-free detection and are particularly interesting due to their simple operation. In this work we report the use of thiol-terminated MIP microspheres to construct surfaces for detection of a model organic analyte, nicotine, by surface enhanced Raman scattering (SERS). The nicotine-imprinted microspheres are synthesized by RAFT precipitation polymerization and converted into thiol-terminated microspheres through aminolysis. The thiol groups on the MIP surface allow the microspheres to be immobilized on a gold-coated substrate. Three different strategies are investigated to achieve surface enhanced Raman scattering in the vicinity of the imprinted sites: (1) direct sputtering of gold nanoparticles, (2) immobilization of gold colloids through the MIPs thiol groups, and (3) trapping of the MIP microspheres in a patterned SERS substrate. For the first time we show that large MIP microspheres can be turned into selective SERS surfaces through the three different approaches of assembly. The MIP-based sensing surfaces are used to detect nicotine to demonstrate the proof of concept. As synthesis and surface functionalization of MIP microspheres and nanoparticles are well established, the methods reported in this work are handy and efficient for constructing label-free chemical sensors, in particular for those based on SERS detection.},
  author       = {Kamra, Tripta and Zhou, Tongchang and Montelius, Lars and Schnadt, Joachim and Ye, Lei},
  issn         = {1520-6882},
  language     = {eng},
  number       = {10},
  pages        = {5056--5061},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Implementation of Molecularly Imprinted Polymer Beads for Surface Enhanced Raman Detection},
  url          = {http://dx.doi.org/10.1021/acs.analchem.5b00774},
  volume       = {87},
  year         = {2015},
}