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Characterization of cyclodextrin glycosyltransferases (CGTases) and their application for synthesis of alkyl glycosides with oligomeric head group

Rather, Younus LU ; Gulshan Kazi, Zubaida LU ; Nordberg Karlsson, Eva LU and Adlercreutz, Patrick LU (2015) In Process Biochemistry 50(5). p.722-728
Abstract
Cyclodextrin glycosyltransferases (CGTases) from Paenibacillus macerans, Thermoanaerobacter sp. ATCC 53627, Bacillus stearothermophilus and a Carboxydocella sp. (phylogenetically identified from genomic DNA) were characterized with respect to their catalytic activity in different reactions, with emphasis on reactions useful for the elongation of the carbohydrate group of alkyl glycosides. All CGTases had activities between 95 and 115 U/mg in the coupling reaction between a-cyclodextrin (alpha-CD) as glucosyl donor and beta-dodecyl maltoside as glucosyl acceptor, but differed very much in the competing hydrolysis of a-CD. The alpha-CD hydrolysis activity ranged from 0.13 U/mg for P. macerans CGTase to 10.5 U/mg for the Carboxydocella sp.... (More)
Cyclodextrin glycosyltransferases (CGTases) from Paenibacillus macerans, Thermoanaerobacter sp. ATCC 53627, Bacillus stearothermophilus and a Carboxydocella sp. (phylogenetically identified from genomic DNA) were characterized with respect to their catalytic activity in different reactions, with emphasis on reactions useful for the elongation of the carbohydrate group of alkyl glycosides. All CGTases had activities between 95 and 115 U/mg in the coupling reaction between a-cyclodextrin (alpha-CD) as glucosyl donor and beta-dodecyl maltoside as glucosyl acceptor, but differed very much in the competing hydrolysis of a-CD. The alpha-CD hydrolysis activity ranged from 0.13 U/mg for P. macerans CGTase to 10.5 U/mg for the Carboxydocella sp. (CspCGT13). Furthermore, the disproportionation activity was much lower for the Paenibacillus CGTase compared to the other CGTases, and consequently this enzyme produced the highest yield of the primary coupling product beta-dodecyl maltooctaoside, which is a valuable surfactant. For production of a polydisperse alkyl glycoside product, disproportionation reactions are useful and the other three CGTases of the current study are efficient catalysts. The newly discovered Carboxydocella sp. (CspCGT13) CGTase has the special feature to produce more of products with even longer carbohydrate groups than the primary coupling product. (C) 2015 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cyclodextrin glycosyltransferase, Disproportionation, Coupling, beta-Dodecyl maltoside, beta-Dodecyl maltooctaoside, alpha-Cyclodextrin
in
Process Biochemistry
volume
50
issue
5
pages
722 - 728
publisher
Elsevier
external identifiers
  • wos:000354147700005
  • scopus:84939980594
ISSN
1873-3298
DOI
10.1016/j.procbio.2015.02.016
language
English
LU publication?
yes
id
87421e1c-d6b4-4e2d-85fd-75ca5f862a1c (old id 7432593)
date added to LUP
2015-06-25 10:13:31
date last changed
2017-09-03 03:27:40
@article{87421e1c-d6b4-4e2d-85fd-75ca5f862a1c,
  abstract     = {Cyclodextrin glycosyltransferases (CGTases) from Paenibacillus macerans, Thermoanaerobacter sp. ATCC 53627, Bacillus stearothermophilus and a Carboxydocella sp. (phylogenetically identified from genomic DNA) were characterized with respect to their catalytic activity in different reactions, with emphasis on reactions useful for the elongation of the carbohydrate group of alkyl glycosides. All CGTases had activities between 95 and 115 U/mg in the coupling reaction between a-cyclodextrin (alpha-CD) as glucosyl donor and beta-dodecyl maltoside as glucosyl acceptor, but differed very much in the competing hydrolysis of a-CD. The alpha-CD hydrolysis activity ranged from 0.13 U/mg for P. macerans CGTase to 10.5 U/mg for the Carboxydocella sp. (CspCGT13). Furthermore, the disproportionation activity was much lower for the Paenibacillus CGTase compared to the other CGTases, and consequently this enzyme produced the highest yield of the primary coupling product beta-dodecyl maltooctaoside, which is a valuable surfactant. For production of a polydisperse alkyl glycoside product, disproportionation reactions are useful and the other three CGTases of the current study are efficient catalysts. The newly discovered Carboxydocella sp. (CspCGT13) CGTase has the special feature to produce more of products with even longer carbohydrate groups than the primary coupling product. (C) 2015 Elsevier Ltd. All rights reserved.},
  author       = {Rather, Younus and Gulshan Kazi, Zubaida and Nordberg Karlsson, Eva and Adlercreutz, Patrick},
  issn         = {1873-3298},
  keyword      = {Cyclodextrin glycosyltransferase,Disproportionation,Coupling,beta-Dodecyl maltoside,beta-Dodecyl maltooctaoside,alpha-Cyclodextrin},
  language     = {eng},
  number       = {5},
  pages        = {722--728},
  publisher    = {Elsevier},
  series       = {Process Biochemistry},
  title        = {Characterization of cyclodextrin glycosyltransferases (CGTases) and their application for synthesis of alkyl glycosides with oligomeric head group},
  url          = {http://dx.doi.org/10.1016/j.procbio.2015.02.016},
  volume       = {50},
  year         = {2015},
}