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Novel anti-apoptotic effect of Bcl-2: Prevention of polyamine depletion-induced cell death

Holst, Martina LU ; Johansson, Veronica LU ; Alm, Kersti LU and Oredsson, Stina LU (2008) In Cell Biology International 32. p.66-74
Abstract
The spermine analogue N(1),N(11)-diethylnorspermine (DENSPM) efficiently depletes the polyamine pools in the breast cancer cell line L56Br-C1 and induces apoptotic cell death via the mitochondrial pathway. In this study, we have over-expressed the anti-apoptotic protein Bcl-2 in L56Br-C1 cells and investigated the effect of DENSPM treatment. DENSPM-induced cell death was significantly reduced in Bcl-2 over-expressing cells. Bcl-2 over-expression reduced DENSPM-induced release of the pro-apoptotic proteins AIF, cytochrome c, and Smac/DIABLO from the mitochondria. Bcl-2 over-expression reduced the DENSPM-induced activation of caspase-3. Bcl-2 over-expression also prevented DENSPM-induced Bax cleavage and reduction of Bcl-X(L) and survivin... (More)
The spermine analogue N(1),N(11)-diethylnorspermine (DENSPM) efficiently depletes the polyamine pools in the breast cancer cell line L56Br-C1 and induces apoptotic cell death via the mitochondrial pathway. In this study, we have over-expressed the anti-apoptotic protein Bcl-2 in L56Br-C1 cells and investigated the effect of DENSPM treatment. DENSPM-induced cell death was significantly reduced in Bcl-2 over-expressing cells. Bcl-2 over-expression reduced DENSPM-induced release of the pro-apoptotic proteins AIF, cytochrome c, and Smac/DIABLO from the mitochondria. Bcl-2 over-expression reduced the DENSPM-induced activation of caspase-3. Bcl-2 over-expression also prevented DENSPM-induced Bax cleavage and reduction of Bcl-X(L) and survivin levels. The DENSPM-induced activation of the polyamine catabolic enzyme spermidine/spermine N(1)-acetyltransferase was reduced by Bcl-2 over-expression, partly preventing polyamine depletion. Thus, Bcl-2 over-expression prevented a number of DENSPM-induced apoptotic effects (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Apoptosis, Spermine analogue, Cytochrome c, Human breast cancer, Bcl-2
in
Cell Biology International
volume
32
pages
66 - 74
publisher
Elsevier
external identifiers
  • wos:000260586200009
  • scopus:39049171988
ISSN
1095-8355
DOI
10.1016/j.cellbi.2007.08.011
language
English
LU publication?
yes
id
9d500204-c53d-41df-9152-232b555aebea (old id 744472)
date added to LUP
2007-12-12 15:16:09
date last changed
2017-07-02 03:30:34
@article{9d500204-c53d-41df-9152-232b555aebea,
  abstract     = {The spermine analogue N(1),N(11)-diethylnorspermine (DENSPM) efficiently depletes the polyamine pools in the breast cancer cell line L56Br-C1 and induces apoptotic cell death via the mitochondrial pathway. In this study, we have over-expressed the anti-apoptotic protein Bcl-2 in L56Br-C1 cells and investigated the effect of DENSPM treatment. DENSPM-induced cell death was significantly reduced in Bcl-2 over-expressing cells. Bcl-2 over-expression reduced DENSPM-induced release of the pro-apoptotic proteins AIF, cytochrome c, and Smac/DIABLO from the mitochondria. Bcl-2 over-expression reduced the DENSPM-induced activation of caspase-3. Bcl-2 over-expression also prevented DENSPM-induced Bax cleavage and reduction of Bcl-X(L) and survivin levels. The DENSPM-induced activation of the polyamine catabolic enzyme spermidine/spermine N(1)-acetyltransferase was reduced by Bcl-2 over-expression, partly preventing polyamine depletion. Thus, Bcl-2 over-expression prevented a number of DENSPM-induced apoptotic effects},
  author       = {Holst, Martina and Johansson, Veronica and Alm, Kersti and Oredsson, Stina},
  issn         = {1095-8355},
  keyword      = {Apoptosis,Spermine analogue,Cytochrome c,Human breast cancer,Bcl-2},
  language     = {eng},
  pages        = {66--74},
  publisher    = {Elsevier},
  series       = {Cell Biology International},
  title        = {Novel anti-apoptotic effect of Bcl-2: Prevention of polyamine depletion-induced cell death},
  url          = {http://dx.doi.org/10.1016/j.cellbi.2007.08.011},
  volume       = {32},
  year         = {2008},
}