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Belimumab concentration measurements using a homologous anti-idiotype immunoassay

Loeff, Floris C. ; Parodis, Ioannis ; Walhelm, Tomas ; Jönsen, Andreas LU ; Nikolopoulos, Dionysis ; Sjöwall, Christopher ; Bengtsson, Anders A. LU ; Kos, Dorien ; van Leeuwen, Astrid and van den Broek, Bryan , et al. (2024) In Journal of Immunological Methods 532.
Abstract

Monitoring belimumab concentrations in patients can be a valuable tool for assessing treatment response and for personalizing drug doses. Various assay formats may be used to measure concentrations of therapeutic monoclonal antibodies. A particularly useful format involves the use of anti-idiotype monoclonal antibodies, selected to be highly specific to the antibody of interest. Here, we describe the development of a specific, high-affinity anti-idiotype antibody to belimumab, and the application of this antibody in a homologous sandwich ELISA to measure belimumab concentrations.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Anti-idiotype antibodies, Belimumab, PK/ADA assays, Rabbit monoclonal antibodies
in
Journal of Immunological Methods
volume
532
article number
113717
publisher
Elsevier
external identifiers
  • scopus:85197474221
  • pmid:38960066
ISSN
0022-1759
DOI
10.1016/j.jim.2024.113717
language
English
LU publication?
yes
id
746960ec-6df4-467b-974a-630b232d62e7
date added to LUP
2024-09-09 15:57:42
date last changed
2025-07-15 20:17:55
@article{746960ec-6df4-467b-974a-630b232d62e7,
  abstract     = {{<p>Monitoring belimumab concentrations in patients can be a valuable tool for assessing treatment response and for personalizing drug doses. Various assay formats may be used to measure concentrations of therapeutic monoclonal antibodies. A particularly useful format involves the use of anti-idiotype monoclonal antibodies, selected to be highly specific to the antibody of interest. Here, we describe the development of a specific, high-affinity anti-idiotype antibody to belimumab, and the application of this antibody in a homologous sandwich ELISA to measure belimumab concentrations.</p>}},
  author       = {{Loeff, Floris C. and Parodis, Ioannis and Walhelm, Tomas and Jönsen, Andreas and Nikolopoulos, Dionysis and Sjöwall, Christopher and Bengtsson, Anders A. and Kos, Dorien and van Leeuwen, Astrid and van den Broek, Bryan and Dijk, Lisanne and Jeremiasse, Jorn and Blomjous, Birgit S. and Voskuyl, Alexandre E. and Wolbink, Gerrit Jan and Bultink, Irene E.M. and Rispens, Theo}},
  issn         = {{0022-1759}},
  keywords     = {{Anti-idiotype antibodies; Belimumab; PK/ADA assays; Rabbit monoclonal antibodies}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Immunological Methods}},
  title        = {{Belimumab concentration measurements using a homologous anti-idiotype immunoassay}},
  url          = {{http://dx.doi.org/10.1016/j.jim.2024.113717}},
  doi          = {{10.1016/j.jim.2024.113717}},
  volume       = {{532}},
  year         = {{2024}},
}