Belimumab concentration measurements using a homologous anti-idiotype immunoassay
(2024) In Journal of Immunological Methods 532.- Abstract
Monitoring belimumab concentrations in patients can be a valuable tool for assessing treatment response and for personalizing drug doses. Various assay formats may be used to measure concentrations of therapeutic monoclonal antibodies. A particularly useful format involves the use of anti-idiotype monoclonal antibodies, selected to be highly specific to the antibody of interest. Here, we describe the development of a specific, high-affinity anti-idiotype antibody to belimumab, and the application of this antibody in a homologous sandwich ELISA to measure belimumab concentrations.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/746960ec-6df4-467b-974a-630b232d62e7
- author
- organization
- publishing date
- 2024-09
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Anti-idiotype antibodies, Belimumab, PK/ADA assays, Rabbit monoclonal antibodies
- in
- Journal of Immunological Methods
- volume
- 532
- article number
- 113717
- publisher
- Elsevier
- external identifiers
-
- pmid:38960066
- scopus:85197474221
- ISSN
- 0022-1759
- DOI
- 10.1016/j.jim.2024.113717
- language
- English
- LU publication?
- yes
- id
- 746960ec-6df4-467b-974a-630b232d62e7
- date added to LUP
- 2024-09-09 15:57:42
- date last changed
- 2024-09-10 11:57:06
@article{746960ec-6df4-467b-974a-630b232d62e7, abstract = {{<p>Monitoring belimumab concentrations in patients can be a valuable tool for assessing treatment response and for personalizing drug doses. Various assay formats may be used to measure concentrations of therapeutic monoclonal antibodies. A particularly useful format involves the use of anti-idiotype monoclonal antibodies, selected to be highly specific to the antibody of interest. Here, we describe the development of a specific, high-affinity anti-idiotype antibody to belimumab, and the application of this antibody in a homologous sandwich ELISA to measure belimumab concentrations.</p>}}, author = {{Loeff, Floris C. and Parodis, Ioannis and Walhelm, Tomas and Jönsen, Andreas and Nikolopoulos, Dionysis and Sjöwall, Christopher and Bengtsson, Anders A. and Kos, Dorien and van Leeuwen, Astrid and van den Broek, Bryan and Dijk, Lisanne and Jeremiasse, Jorn and Blomjous, Birgit S. and Voskuyl, Alexandre E. and Wolbink, Gerrit Jan and Bultink, Irene E.M. and Rispens, Theo}}, issn = {{0022-1759}}, keywords = {{Anti-idiotype antibodies; Belimumab; PK/ADA assays; Rabbit monoclonal antibodies}}, language = {{eng}}, publisher = {{Elsevier}}, series = {{Journal of Immunological Methods}}, title = {{Belimumab concentration measurements using a homologous anti-idiotype immunoassay}}, url = {{http://dx.doi.org/10.1016/j.jim.2024.113717}}, doi = {{10.1016/j.jim.2024.113717}}, volume = {{532}}, year = {{2024}}, }