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Somatostatin effects on the proteome of the LNCaP cell-line

Liu, Zhaoxu; Bengtsson, Sofia; Krogh, Morten LU ; Marquez, M; Nilsson, S; James, Peter LU ; Alaiya, A and Holmberg, Anders (2007) In International Journal of Oncology 30(5). p.1173-1179
Abstract
Some clinical results indicate that somatostatin (sms) might be useful in the treatment of advanced prostate cancer (HRPC). Because of its transient in vivo half-life only more stable derivatives of sms are of interest in this context. Recent studies have shown that natural sms can be conjugated to a carbohydrate (smsdx) with preservation of sms-like effects on the prostatic tumor cell proteome. The present study identifies some of the affected proteins in an effort to elucidate pathways and proteins that might be of importance for the potential usefulness of sms treatment in HRPC. After incubating the LNCaP cell-line with sms14/smsdx, comparative proteomics was used for analysing and identifying affected proteins. Protein expression... (More)
Some clinical results indicate that somatostatin (sms) might be useful in the treatment of advanced prostate cancer (HRPC). Because of its transient in vivo half-life only more stable derivatives of sms are of interest in this context. Recent studies have shown that natural sms can be conjugated to a carbohydrate (smsdx) with preservation of sms-like effects on the prostatic tumor cell proteome. The present study identifies some of the affected proteins in an effort to elucidate pathways and proteins that might be of importance for the potential usefulness of sms treatment in HRPC. After incubating the LNCaP cell-line with sms14/smsdx, comparative proteomics was used for analysing and identifying affected proteins. Protein expression patterns were analysed with two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Catalytic mitochondrial and mitochondrial-associated proteins were significantly affected (fold change approximately 2 or higher) and they were in general up-regulated. Apoptosis-related proteins were both up-regulated (VDAC1, VDAC2) and down-regulated (PRDX2, TCTP). The fold change was >2 for PRDX2 and <2 for the others. There was a strong agreement between sms and smsdx on the up- and down-regulation of proteins. Sms/smsdx triggered up-regulation of catalytic mitochondrial proteins and seemed to affect apoptosis-related proteins. This could indicate important pathways on which smsdx might be able to curb the progression of HRPC. The results encourage a pending clinical phase II study. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Oncology
volume
30
issue
5
pages
1173 - 1179
publisher
D.A. Spandidos
external identifiers
  • wos:000245903900015
  • scopus:34447329624
ISSN
1019-6439
DOI
10.3892/ijo.30.5.1173
language
English
LU publication?
yes
id
9d16ff63-06d8-480b-96ab-f25c7f40bc46 (old id 747289)
date added to LUP
2007-12-17 09:41:53
date last changed
2017-05-21 04:20:50
@article{9d16ff63-06d8-480b-96ab-f25c7f40bc46,
  abstract     = {Some clinical results indicate that somatostatin (sms) might be useful in the treatment of advanced prostate cancer (HRPC). Because of its transient in vivo half-life only more stable derivatives of sms are of interest in this context. Recent studies have shown that natural sms can be conjugated to a carbohydrate (smsdx) with preservation of sms-like effects on the prostatic tumor cell proteome. The present study identifies some of the affected proteins in an effort to elucidate pathways and proteins that might be of importance for the potential usefulness of sms treatment in HRPC. After incubating the LNCaP cell-line with sms14/smsdx, comparative proteomics was used for analysing and identifying affected proteins. Protein expression patterns were analysed with two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Catalytic mitochondrial and mitochondrial-associated proteins were significantly affected (fold change approximately 2 or higher) and they were in general up-regulated. Apoptosis-related proteins were both up-regulated (VDAC1, VDAC2) and down-regulated (PRDX2, TCTP). The fold change was &gt;2 for PRDX2 and &lt;2 for the others. There was a strong agreement between sms and smsdx on the up- and down-regulation of proteins. Sms/smsdx triggered up-regulation of catalytic mitochondrial proteins and seemed to affect apoptosis-related proteins. This could indicate important pathways on which smsdx might be able to curb the progression of HRPC. The results encourage a pending clinical phase II study.},
  author       = {Liu, Zhaoxu and Bengtsson, Sofia and Krogh, Morten and Marquez, M and Nilsson, S and James, Peter and Alaiya, A and Holmberg, Anders},
  issn         = {1019-6439},
  language     = {eng},
  number       = {5},
  pages        = {1173--1179},
  publisher    = {D.A. Spandidos},
  series       = {International Journal of Oncology},
  title        = {Somatostatin effects on the proteome of the LNCaP cell-line},
  url          = {http://dx.doi.org/10.3892/ijo.30.5.1173},
  volume       = {30},
  year         = {2007},
}