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Passenger strand loading in overexpression experiments using microRNA mimics.

Sökilde, Rolf LU ; Newie, Inga LU ; Persson, Helena LU ; Borg, Åke LU and Rovira, Carlos LU (2015) In RNA Biology 12(8). p.787-791
Abstract
MicroRNAs (miRNAs) are important regulators of gene function and manipulation of miRNAs is a central component of basic research. Modulation of gene expression by miRNA gain-of-function can be based on different approaches including transfection with miRNA mimics; artificial, chemically modified miRNA-like small RNAs. These molecules are intended to mimic the function of a miRNA guide strand while bypassing the maturation steps of endogenous miRNAs. Due to easy accessibility through commercial providers this approach has gained popularity, and accuracy is often assumed without prior independent testing. Our in silico analysis of over-represented sequence motifs in microarray expression data and sequencing of AGO-associated small RNAs... (More)
MicroRNAs (miRNAs) are important regulators of gene function and manipulation of miRNAs is a central component of basic research. Modulation of gene expression by miRNA gain-of-function can be based on different approaches including transfection with miRNA mimics; artificial, chemically modified miRNA-like small RNAs. These molecules are intended to mimic the function of a miRNA guide strand while bypassing the maturation steps of endogenous miRNAs. Due to easy accessibility through commercial providers this approach has gained popularity, and accuracy is often assumed without prior independent testing. Our in silico analysis of over-represented sequence motifs in microarray expression data and sequencing of AGO-associated small RNAs indicate, however, that miRNA mimics may be associated with considerable side-effects due to the unwanted activity of the miRNA mimic complementary strand. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
RNA Biology
volume
12
issue
8
pages
787 - 791
publisher
Taylor & Francis
external identifiers
  • pmid:26121563
  • wos:000359659700003
  • scopus:84944457309
ISSN
1547-6286
DOI
10.1080/15476286.2015.1020270
project
CREATE Health
language
English
LU publication?
yes
id
a67fe792-c865-44a2-bee2-072db0a3748b (old id 7477349)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26121563?dopt=Abstract
date added to LUP
2015-07-11 10:32:12
date last changed
2017-11-12 03:08:33
@article{a67fe792-c865-44a2-bee2-072db0a3748b,
  abstract     = {MicroRNAs (miRNAs) are important regulators of gene function and manipulation of miRNAs is a central component of basic research. Modulation of gene expression by miRNA gain-of-function can be based on different approaches including transfection with miRNA mimics; artificial, chemically modified miRNA-like small RNAs. These molecules are intended to mimic the function of a miRNA guide strand while bypassing the maturation steps of endogenous miRNAs. Due to easy accessibility through commercial providers this approach has gained popularity, and accuracy is often assumed without prior independent testing. Our in silico analysis of over-represented sequence motifs in microarray expression data and sequencing of AGO-associated small RNAs indicate, however, that miRNA mimics may be associated with considerable side-effects due to the unwanted activity of the miRNA mimic complementary strand.},
  author       = {Sökilde, Rolf and Newie, Inga and Persson, Helena and Borg, Åke and Rovira, Carlos},
  issn         = {1547-6286},
  language     = {eng},
  number       = {8},
  pages        = {787--791},
  publisher    = {Taylor & Francis},
  series       = {RNA Biology},
  title        = {Passenger strand loading in overexpression experiments using microRNA mimics.},
  url          = {http://dx.doi.org/10.1080/15476286.2015.1020270},
  volume       = {12},
  year         = {2015},
}