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Thermomyces lanuginosus lipase-catalyzed hydrolysis of the lipid cubic liquid crystalline nanoparticles.

Barauskas, Justas; Anderberg, Hanna LU ; Svendsen, Allan and Nylander, Tommy LU (2016) In Colloids and Surfaces B: Biointerfaces 137(Online 08 May 2015). p.50-59
Abstract
In this study well-ordered glycerol monooleate (GMO)-based cubic liquid crystalline nanoparticles (LCNPs) have been used as substrates for Thermomyces lanuginosus lipase in order to establish the relation between the catalytic activity, measured by pH-stat titration, and the change in morphology and nanostructure determined by cryogenic transmission electron microscopy and synchrotron small angle X-ray diffraction. The initial lipase catalyzed LCNP hydrolysis rate is approximately 25% higher for large 350nm nanoparticles compared to the small 190nm particles, which is attributed to the increased number of structural defects on the particle surface. At pH 8.0 and 8.4 bicontinuous Im3m cubic LCNPs transform into "sponge"-like assemblies and... (More)
In this study well-ordered glycerol monooleate (GMO)-based cubic liquid crystalline nanoparticles (LCNPs) have been used as substrates for Thermomyces lanuginosus lipase in order to establish the relation between the catalytic activity, measured by pH-stat titration, and the change in morphology and nanostructure determined by cryogenic transmission electron microscopy and synchrotron small angle X-ray diffraction. The initial lipase catalyzed LCNP hydrolysis rate is approximately 25% higher for large 350nm nanoparticles compared to the small 190nm particles, which is attributed to the increased number of structural defects on the particle surface. At pH 8.0 and 8.4 bicontinuous Im3m cubic LCNPs transform into "sponge"-like assemblies and disordered multilamellar onion-like structures upon exposure to lipase. At pH 6.5 and 7.5 lipolysis induced phase transitions of the inner core of the particles, following the sequence Im3m cubic → reversed hexagonal → reversed micellar Fd3m cubic → reversed micelles. These transitions to the liquid crystalline phases with higher negative curvature of the lipid/water interface were found to trigger protonation of the oleic acid produced during lipase catalyzed reaction. The increase curvature of the reversed discrete micellar cubic phase was suggested to cause an increase in the oleic acid pKa to a larger value observed by pH-stat titration. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Colloids and Surfaces B: Biointerfaces
volume
137
issue
Online 08 May 2015
pages
50 - 59
publisher
Elsevier
external identifiers
  • pmid:26047576
  • wos:000367491200007
  • scopus:84951574015
ISSN
1873-4367
DOI
10.1016/j.colsurfb.2015.04.052
language
English
LU publication?
yes
id
7b33b505-4144-44a0-9264-b6dda7502099 (old id 7488118)
date added to LUP
2015-07-08 11:35:09
date last changed
2017-09-24 03:18:19
@article{7b33b505-4144-44a0-9264-b6dda7502099,
  abstract     = {In this study well-ordered glycerol monooleate (GMO)-based cubic liquid crystalline nanoparticles (LCNPs) have been used as substrates for Thermomyces lanuginosus lipase in order to establish the relation between the catalytic activity, measured by pH-stat titration, and the change in morphology and nanostructure determined by cryogenic transmission electron microscopy and synchrotron small angle X-ray diffraction. The initial lipase catalyzed LCNP hydrolysis rate is approximately 25% higher for large 350nm nanoparticles compared to the small 190nm particles, which is attributed to the increased number of structural defects on the particle surface. At pH 8.0 and 8.4 bicontinuous Im3m cubic LCNPs transform into "sponge"-like assemblies and disordered multilamellar onion-like structures upon exposure to lipase. At pH 6.5 and 7.5 lipolysis induced phase transitions of the inner core of the particles, following the sequence Im3m cubic → reversed hexagonal → reversed micellar Fd3m cubic → reversed micelles. These transitions to the liquid crystalline phases with higher negative curvature of the lipid/water interface were found to trigger protonation of the oleic acid produced during lipase catalyzed reaction. The increase curvature of the reversed discrete micellar cubic phase was suggested to cause an increase in the oleic acid pKa to a larger value observed by pH-stat titration.},
  author       = {Barauskas, Justas and Anderberg, Hanna and Svendsen, Allan and Nylander, Tommy},
  issn         = {1873-4367},
  language     = {eng},
  number       = {Online 08 May 2015},
  pages        = {50--59},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces B: Biointerfaces},
  title        = {Thermomyces lanuginosus lipase-catalyzed hydrolysis of the lipid cubic liquid crystalline nanoparticles.},
  url          = {http://dx.doi.org/10.1016/j.colsurfb.2015.04.052},
  volume       = {137},
  year         = {2016},
}