Comparing Analysis Methods in Functional Calcium Imaging of the Insect Brain.
Balkenius, Anna LU ; Johansson, Anders J LU
and Balkenius, Christian
LU
(2015)
In PLoS ONE
10(6).
- Abstract
- We investigate four different methods for background estimation in calcium imaging of the insect brain and evaluate their performance on six data sets consisting of data recorded from two sites in two species of moths. The calcium fluorescence decay curve outside the potential response is estimated using either a low-pass filter or constant, linear or polynomial regression, and is subsequently used to calculate the magnitude, latency and duration of the response. The magnitude and variance of the responses that are obtained by the different methods are compared, and, by computing the receiver operating characteristics of a classifier based on response magnitude, we evaluate the ability of each method to detect the stimulus type and... (More)
- We investigate four different methods for background estimation in calcium imaging of the insect brain and evaluate their performance on six data sets consisting of data recorded from two sites in two species of moths. The calcium fluorescence decay curve outside the potential response is estimated using either a low-pass filter or constant, linear or polynomial regression, and is subsequently used to calculate the magnitude, latency and duration of the response. The magnitude and variance of the responses that are obtained by the different methods are compared, and, by computing the receiver operating characteristics of a classifier based on response magnitude, we evaluate the ability of each method to detect the stimulus type and conclude that a polynomial approximation of the background gives the overall best result. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7488159
- author
- Balkenius, Anna
LU
; Johansson, Anders J
LU
and Balkenius, Christian
LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- PLoS ONE
- volume
- 10
- issue
- 6
- article number
- e0129614
- publisher
- Public Library of Science (PLoS)
- external identifiers
-
- pmid:26046538
- wos:000355652200164
- scopus:84934958950
- pmid:26046538
- ISSN
- 1932-6203
- DOI
- 10.1371/journal.pone.0129614
- language
- English
- LU publication?
- yes
- id
- 9da1ec9c-70dc-4a78-899a-7697a6ef44a5 (old id 7488159)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/26046538?dopt=Abstract
- date added to LUP
- 2016-04-01 14:49:10
- date last changed
- 2024-04-16 02:43:56
@article{9da1ec9c-70dc-4a78-899a-7697a6ef44a5,
abstract = {{We investigate four different methods for background estimation in calcium imaging of the insect brain and evaluate their performance on six data sets consisting of data recorded from two sites in two species of moths. The calcium fluorescence decay curve outside the potential response is estimated using either a low-pass filter or constant, linear or polynomial regression, and is subsequently used to calculate the magnitude, latency and duration of the response. The magnitude and variance of the responses that are obtained by the different methods are compared, and, by computing the receiver operating characteristics of a classifier based on response magnitude, we evaluate the ability of each method to detect the stimulus type and conclude that a polynomial approximation of the background gives the overall best result.}},
author = {{Balkenius, Anna and Johansson, Anders J and Balkenius, Christian}},
issn = {{1932-6203}},
language = {{eng}},
number = {{6}},
publisher = {{Public Library of Science (PLoS)}},
series = {{PLoS ONE}},
title = {{Comparing Analysis Methods in Functional Calcium Imaging of the Insect Brain.}},
url = {{http://dx.doi.org/10.1371/journal.pone.0129614}},
doi = {{10.1371/journal.pone.0129614}},
volume = {{10}},
year = {{2015}},
}