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Comparing Analysis Methods in Functional Calcium Imaging of the Insect Brain.

Balkenius, Anna; Johansson, Anders J LU and Balkenius, Christian LU (2015) In PLoS ONE 10(6).
Abstract
We investigate four different methods for background estimation in calcium imaging of the insect brain and evaluate their performance on six data sets consisting of data recorded from two sites in two species of moths. The calcium fluorescence decay curve outside the potential response is estimated using either a low-pass filter or constant, linear or polynomial regression, and is subsequently used to calculate the magnitude, latency and duration of the response. The magnitude and variance of the responses that are obtained by the different methods are compared, and, by computing the receiver operating characteristics of a classifier based on response magnitude, we evaluate the ability of each method to detect the stimulus type and... (More)
We investigate four different methods for background estimation in calcium imaging of the insect brain and evaluate their performance on six data sets consisting of data recorded from two sites in two species of moths. The calcium fluorescence decay curve outside the potential response is estimated using either a low-pass filter or constant, linear or polynomial regression, and is subsequently used to calculate the magnitude, latency and duration of the response. The magnitude and variance of the responses that are obtained by the different methods are compared, and, by computing the receiver operating characteristics of a classifier based on response magnitude, we evaluate the ability of each method to detect the stimulus type and conclude that a polynomial approximation of the background gives the overall best result. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
10
issue
6
publisher
Public Library of Science
external identifiers
  • pmid:26046538
  • wos:000355652200164
  • scopus:84934958950
ISSN
1932-6203
DOI
10.1371/journal.pone.0129614
language
English
LU publication?
yes
id
9da1ec9c-70dc-4a78-899a-7697a6ef44a5 (old id 7488159)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26046538?dopt=Abstract
date added to LUP
2015-07-06 16:39:33
date last changed
2017-07-30 04:21:27
@article{9da1ec9c-70dc-4a78-899a-7697a6ef44a5,
  abstract     = {We investigate four different methods for background estimation in calcium imaging of the insect brain and evaluate their performance on six data sets consisting of data recorded from two sites in two species of moths. The calcium fluorescence decay curve outside the potential response is estimated using either a low-pass filter or constant, linear or polynomial regression, and is subsequently used to calculate the magnitude, latency and duration of the response. The magnitude and variance of the responses that are obtained by the different methods are compared, and, by computing the receiver operating characteristics of a classifier based on response magnitude, we evaluate the ability of each method to detect the stimulus type and conclude that a polynomial approximation of the background gives the overall best result.},
  articleno    = {e0129614},
  author       = {Balkenius, Anna and Johansson, Anders J and Balkenius, Christian},
  issn         = {1932-6203},
  language     = {eng},
  number       = {6},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {Comparing Analysis Methods in Functional Calcium Imaging of the Insect Brain.},
  url          = {http://dx.doi.org/10.1371/journal.pone.0129614},
  volume       = {10},
  year         = {2015},
}