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Probing hematopoietic stem cell function using serial transplantation: Seeding characteristics and the impact of stem cell purification.

Rundberg Nilsson, Alexandra LU ; Pronk, Kees-Jan LU and Bryder, David LU (2015) In Experimental Hematology 43(9). p.812-817
Abstract
Appropriate regulation of hematopoietic stem cell (HSC) numbers and function is a requisite for life-long blood cell replenishment. Knowledge of factors that regulate HSC activity is derived largely from murine model systems, with serial transplantation often considered a "gold standard" to assess longevity and self-renewal of HSCs. In the literature, we noted inconsistencies in how serial transplantations are conducted and decided to assess a set of parameters at play in such experiments. We found that HSCs distribute and expand unevenly among individual bones following transplantation, suggesting that isolation of a limited number of bone marrow cells for serial transplantation and/or analysis can influence experimental outcomes.... (More)
Appropriate regulation of hematopoietic stem cell (HSC) numbers and function is a requisite for life-long blood cell replenishment. Knowledge of factors that regulate HSC activity is derived largely from murine model systems, with serial transplantation often considered a "gold standard" to assess longevity and self-renewal of HSCs. In the literature, we noted inconsistencies in how serial transplantations are conducted and decided to assess a set of parameters at play in such experiments. We found that HSCs distribute and expand unevenly among individual bones following transplantation, suggesting that isolation of a limited number of bone marrow cells for serial transplantation and/or analysis can influence experimental outcomes. Comparing donor cell output from transplanted unfractionated bone marrow cells, as opposed to fluorescence-activated cell-sorted HSCs, revealed distinct differences in the output of mature blood cells. Specifically, we found that long-lived progenitor and/or mature co-transplanted cells can severely affect the interpretation of ongoing HSC activity in secondary hosts. The implications of these data for the design and execution of serial transplantation experiments are discussed. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Experimental Hematology
volume
43
issue
9
pages
812 - 817
publisher
Elsevier
external identifiers
  • pmid:26027791
  • wos:000361417200009
  • scopus:84940452324
ISSN
1873-2399
DOI
10.1016/j.exphem.2015.05.003
language
English
LU publication?
yes
id
3db2425c-aa4a-4be0-a6c5-1d1caa54967d (old id 7508651)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26027791?dopt=Abstract
date added to LUP
2015-07-05 06:17:04
date last changed
2017-07-23 03:29:02
@article{3db2425c-aa4a-4be0-a6c5-1d1caa54967d,
  abstract     = {Appropriate regulation of hematopoietic stem cell (HSC) numbers and function is a requisite for life-long blood cell replenishment. Knowledge of factors that regulate HSC activity is derived largely from murine model systems, with serial transplantation often considered a "gold standard" to assess longevity and self-renewal of HSCs. In the literature, we noted inconsistencies in how serial transplantations are conducted and decided to assess a set of parameters at play in such experiments. We found that HSCs distribute and expand unevenly among individual bones following transplantation, suggesting that isolation of a limited number of bone marrow cells for serial transplantation and/or analysis can influence experimental outcomes. Comparing donor cell output from transplanted unfractionated bone marrow cells, as opposed to fluorescence-activated cell-sorted HSCs, revealed distinct differences in the output of mature blood cells. Specifically, we found that long-lived progenitor and/or mature co-transplanted cells can severely affect the interpretation of ongoing HSC activity in secondary hosts. The implications of these data for the design and execution of serial transplantation experiments are discussed.},
  author       = {Rundberg Nilsson, Alexandra and Pronk, Kees-Jan and Bryder, David},
  issn         = {1873-2399},
  language     = {eng},
  number       = {9},
  pages        = {812--817},
  publisher    = {Elsevier},
  series       = {Experimental Hematology},
  title        = {Probing hematopoietic stem cell function using serial transplantation: Seeding characteristics and the impact of stem cell purification.},
  url          = {http://dx.doi.org/10.1016/j.exphem.2015.05.003},
  volume       = {43},
  year         = {2015},
}