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Increased levels of mitogen activated protein kinase (MAP-K) detected in the injured adult mouse sciatic nerve

Svensson, Bodil; Ekström, Per LU and Edström, Anders LU (1995) In Neuroscience Letters 200(1). p.33-36
Abstract

Adult mouse sciatic nerves (SNs) with attached dorsal root ganglia (DRG) were analysed for the presence of mitogen activated protein kinase (MAP-K) during normal and regenerative conditions. By immunohistochemistry, MAP-k was found to be present in the normal nerve at low levels in both Schwann cells and DRG nerve cell bodies, with a profoundly increased expression during regeneration. In axonal outgrowth assays, treatment with 2 mM 2-aminopurine (2-AP), a MAP-K antagonist, inhibited the regeneration of axons from the SN as well as from the cultured superior cervical ganglia. The reduced outgrowth was probably not due to toxic effects of the drug since the ganglionic protein synthesis was not inhibited. It is possible that 2-AP... (More)

Adult mouse sciatic nerves (SNs) with attached dorsal root ganglia (DRG) were analysed for the presence of mitogen activated protein kinase (MAP-K) during normal and regenerative conditions. By immunohistochemistry, MAP-k was found to be present in the normal nerve at low levels in both Schwann cells and DRG nerve cell bodies, with a profoundly increased expression during regeneration. In axonal outgrowth assays, treatment with 2 mM 2-aminopurine (2-AP), a MAP-K antagonist, inhibited the regeneration of axons from the SN as well as from the cultured superior cervical ganglia. The reduced outgrowth was probably not due to toxic effects of the drug since the ganglionic protein synthesis was not inhibited. It is possible that 2-AP inteferes with regeneration-related events by inhibition of MAP-K.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
2-Aminopurine, Adult, Mitogen activated protein kinase, Mouse, Nerve regeneration, Sciatic nerve
in
Neuroscience Letters
volume
200
issue
1
pages
4 pages
publisher
Elsevier
external identifiers
  • scopus:0028805095
ISSN
0304-3940
DOI
10.1016/0304-3940(95)12066-D
language
English
LU publication?
yes
id
759836ee-da97-438a-aab2-34af497f5bd9
date added to LUP
2016-12-07 14:52:47
date last changed
2017-01-20 16:31:54
@article{759836ee-da97-438a-aab2-34af497f5bd9,
  abstract     = {<p>Adult mouse sciatic nerves (SNs) with attached dorsal root ganglia (DRG) were analysed for the presence of mitogen activated protein kinase (MAP-K) during normal and regenerative conditions. By immunohistochemistry, MAP-k was found to be present in the normal nerve at low levels in both Schwann cells and DRG nerve cell bodies, with a profoundly increased expression during regeneration. In axonal outgrowth assays, treatment with 2 mM 2-aminopurine (2-AP), a MAP-K antagonist, inhibited the regeneration of axons from the SN as well as from the cultured superior cervical ganglia. The reduced outgrowth was probably not due to toxic effects of the drug since the ganglionic protein synthesis was not inhibited. It is possible that 2-AP inteferes with regeneration-related events by inhibition of MAP-K.</p>},
  author       = {Svensson, Bodil and Ekström, Per and Edström, Anders},
  issn         = {0304-3940},
  keyword      = {2-Aminopurine,Adult,Mitogen activated protein kinase,Mouse,Nerve regeneration,Sciatic nerve},
  language     = {eng},
  month        = {11},
  number       = {1},
  pages        = {33--36},
  publisher    = {Elsevier},
  series       = {Neuroscience Letters},
  title        = {Increased levels of mitogen activated protein kinase (MAP-K) detected in the injured adult mouse sciatic nerve},
  url          = {http://dx.doi.org/10.1016/0304-3940(95)12066-D},
  volume       = {200},
  year         = {1995},
}