Advanced

Rat amnion membrane matrix as a substratum for regenerating axons from peripheral and central neurons : effects in a silicone chamber model

Danielsen, N LU ; Müller, H; Pettmann, Brigitte; Williams, L R; Davis, G E; Engvall, E; Manthorpe, M and Varon, S (1988) In Developmental Brain Research 39(1). p.39-50
Abstract

An extracellular matrix preparation, the human amnion membrane matrix (hAMM) can serve as a neurite-promoting substratum for cultured peripheral and central neurons, and also as a support for axonal growth in experimentally injured adult brain in vivo. In the present study, we tested similar materials as bridges in a silicone chamber model for the regeneration of sciatic nerve in the adult rat. Since hAMM elicited an inflammatory response, we developed a rat amnion membrane matrix (rAMM), which proved to be an excellent neurite-promoting substratum for cultured ganglionic and spinal cord neurons. The rAMM was coiled and inserted in the 10 mm gap between the two nerve stumps from the silicone chambers. At 16 days after implantation,... (More)

An extracellular matrix preparation, the human amnion membrane matrix (hAMM) can serve as a neurite-promoting substratum for cultured peripheral and central neurons, and also as a support for axonal growth in experimentally injured adult brain in vivo. In the present study, we tested similar materials as bridges in a silicone chamber model for the regeneration of sciatic nerve in the adult rat. Since hAMM elicited an inflammatory response, we developed a rat amnion membrane matrix (rAMM), which proved to be an excellent neurite-promoting substratum for cultured ganglionic and spinal cord neurons. The rAMM was coiled and inserted in the 10 mm gap between the two nerve stumps from the silicone chambers. At 16 days after implantation, temporal progress of regeneration was grossly similar as in saline-prefilled control chambers. However, rAMM-prefilled chambers displayed significantly higher number of vessels and a markedly different geometry of the regenerate: an endoneurium, surrounded by a perineurial-like cell layer, was formed outside the largely preserved central portion of the rAMM coil. After longer regeneration times (28 days), a rAMM core was no longer detected, but some rAMM-like materials remained interspersed in the endoneurium. The overall organization of the regenerate and the number of myelinated axons at this time were similar to those of control chambers, although the endoneurial cross-sectional area was larger in the rAMM chambers. One specimen, however, displayed the very patterns for which the experiments were designed, namely an array of numerous, myelinated axons tracing the spiraling spaces between consecutive lamellae of the rAMM coil.

(Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amnion, Animals, Axons, Brain, Cells, Cultured, Chick Embryo, Culture Techniques, Humans, Laminin, Nerve Regeneration, Peripheral Nerves, Rats, Rats, Inbred Strains, Silicones, Time Factors, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.
in
Developmental Brain Research
volume
39
issue
1
pages
12 pages
publisher
Elsevier
external identifiers
  • scopus:0023974223
ISSN
0165-3806
DOI
10.1016/0165-3806(88)90065-X
language
English
LU publication?
no
id
75c1a2d7-45fb-4693-8512-edac70ca32e6
date added to LUP
2017-10-13 13:43:21
date last changed
2017-11-29 13:28:53
@article{75c1a2d7-45fb-4693-8512-edac70ca32e6,
  abstract     = {<p>An extracellular matrix preparation, the human amnion membrane matrix (hAMM) can serve as a neurite-promoting substratum for cultured peripheral and central neurons, and also as a support for axonal growth in experimentally injured adult brain in vivo. In the present study, we tested similar materials as bridges in a silicone chamber model for the regeneration of sciatic nerve in the adult rat. Since hAMM elicited an inflammatory response, we developed a rat amnion membrane matrix (rAMM), which proved to be an excellent neurite-promoting substratum for cultured ganglionic and spinal cord neurons. The rAMM was coiled and inserted in the 10 mm gap between the two nerve stumps from the silicone chambers. At 16 days after implantation, temporal progress of regeneration was grossly similar as in saline-prefilled control chambers. However, rAMM-prefilled chambers displayed significantly higher number of vessels and a markedly different geometry of the regenerate: an endoneurium, surrounded by a perineurial-like cell layer, was formed outside the largely preserved central portion of the rAMM coil. After longer regeneration times (28 days), a rAMM core was no longer detected, but some rAMM-like materials remained interspersed in the endoneurium. The overall organization of the regenerate and the number of myelinated axons at this time were similar to those of control chambers, although the endoneurial cross-sectional area was larger in the rAMM chambers. One specimen, however, displayed the very patterns for which the experiments were designed, namely an array of numerous, myelinated axons tracing the spiraling spaces between consecutive lamellae of the rAMM coil.</p>},
  author       = {Danielsen, N and Müller, H and Pettmann, Brigitte and Williams, L R and Davis, G E and Engvall, E and Manthorpe, M and Varon, S},
  issn         = {0165-3806},
  keyword      = {Amnion,Animals,Axons,Brain,Cells, Cultured,Chick Embryo,Culture Techniques,Humans,Laminin,Nerve Regeneration,Peripheral Nerves,Rats,Rats, Inbred Strains,Silicones,Time Factors,Journal Article,Research Support, Non-U.S. Gov't,Research Support, U.S. Gov't, Non-P.H.S.,Research Support, U.S. Gov't, P.H.S.},
  language     = {eng},
  month        = {03},
  number       = {1},
  pages        = {39--50},
  publisher    = {Elsevier},
  series       = {Developmental Brain Research},
  title        = {Rat amnion membrane matrix as a substratum for regenerating axons from peripheral and central neurons : effects in a silicone chamber model},
  url          = {http://dx.doi.org/10.1016/0165-3806(88)90065-X},
  volume       = {39},
  year         = {1988},
}