Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Chlorhexidine for prevention of neonatal colonization with group B streptococci. I. In vitro effect of chlorhexidine on group B streptococci

Kvist Christensen, Karen ; Christensen, Poul ; Dykes, Anna-Karin LU ; Kahlmeter, Gunnar ; Kurl, Daya N and Linden, Viveka (1983) In European Journal of Obstetrics, Gynecology, and Reproductive Biology 16(3). p.157-165
Abstract
Forty-three strains of group B streptococci (GBS) of types Ia, Ib, II and III were tested for susceptibility to chlorhexidine in concentrations ranging from 256 to 0.25 mg/l using the agar and tube dilution methods. The strains showed minimum inhibitory concentration (MIC) values ranging from 0.5 to 1 mg/l. Serum added to the test medium (50%) increased the MIC values to 4-8 mg/l, while amniotic fluid (50%) had almost no effect, increasing the values to 1-2 mg/l. The minimum bactericidal concentration (MBC) ranged from 1 to 5 mg/l. The killing kinetics were related to the concentration of chlorhexidine and the length of exposure. For example, at a concentration of 63 mg/l, 7 h were required for a bactericidal effect in broth, as compared... (More)
Forty-three strains of group B streptococci (GBS) of types Ia, Ib, II and III were tested for susceptibility to chlorhexidine in concentrations ranging from 256 to 0.25 mg/l using the agar and tube dilution methods. The strains showed minimum inhibitory concentration (MIC) values ranging from 0.5 to 1 mg/l. Serum added to the test medium (50%) increased the MIC values to 4-8 mg/l, while amniotic fluid (50%) had almost no effect, increasing the values to 1-2 mg/l. The minimum bactericidal concentration (MBC) ranged from 1 to 5 mg/l. The killing kinetics were related to the concentration of chlorhexidine and the length of exposure. For example, at a concentration of 63 mg/l, 7 h were required for a bactericidal effect in broth, as compared to 1 h at 500 mg/l chlorhexidine. 200 mg/l chlordexidine had no effect on the adherence of two GBS strains to vaginal epithelial cells, and no effect on the phagocytosis of GBS with mouse peritoneal macrophages. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
group B streptococci, chlorhexidine, minimal inhibitory concentration, minimal bactericidal concentration, adherence, phagocytosis
in
European Journal of Obstetrics, Gynecology, and Reproductive Biology
volume
16
issue
3
pages
157 - 165
publisher
Elsevier
external identifiers
  • pmid:6363151
  • scopus:0021075141
ISSN
0301-2115
DOI
10.1016/0028-2243(83)90095-3
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Nursing (Closed 2012) (013065000), Department of Obstetrics and Gynaecology (Lund) (013018000), Division of Medical Microbiology (013250400)
id
75ee651c-3d2d-4b31-85e3-c2882b080582 (old id 1103144)
date added to LUP
2016-04-01 12:24:16
date last changed
2021-09-19 03:10:16
@article{75ee651c-3d2d-4b31-85e3-c2882b080582,
  abstract     = {{Forty-three strains of group B streptococci (GBS) of types Ia, Ib, II and III were tested for susceptibility to chlorhexidine in concentrations ranging from 256 to 0.25 mg/l using the agar and tube dilution methods. The strains showed minimum inhibitory concentration (MIC) values ranging from 0.5 to 1 mg/l. Serum added to the test medium (50%) increased the MIC values to 4-8 mg/l, while amniotic fluid (50%) had almost no effect, increasing the values to 1-2 mg/l. The minimum bactericidal concentration (MBC) ranged from 1 to 5 mg/l. The killing kinetics were related to the concentration of chlorhexidine and the length of exposure. For example, at a concentration of 63 mg/l, 7 h were required for a bactericidal effect in broth, as compared to 1 h at 500 mg/l chlorhexidine. 200 mg/l chlordexidine had no effect on the adherence of two GBS strains to vaginal epithelial cells, and no effect on the phagocytosis of GBS with mouse peritoneal macrophages.}},
  author       = {{Kvist Christensen, Karen and Christensen, Poul and Dykes, Anna-Karin and Kahlmeter, Gunnar and Kurl, Daya N and Linden, Viveka}},
  issn         = {{0301-2115}},
  keywords     = {{group B streptococci; chlorhexidine; minimal inhibitory concentration; minimal bactericidal concentration; adherence; phagocytosis}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{157--165}},
  publisher    = {{Elsevier}},
  series       = {{European Journal of Obstetrics, Gynecology, and Reproductive Biology}},
  title        = {{Chlorhexidine for prevention of neonatal colonization with group B streptococci. I. In vitro effect of chlorhexidine on group B streptococci}},
  url          = {{http://dx.doi.org/10.1016/0028-2243(83)90095-3}},
  doi          = {{10.1016/0028-2243(83)90095-3}},
  volume       = {{16}},
  year         = {{1983}},
}