Chlorhexidine for prevention of neonatal colonization with group B streptococci. I. In vitro effect of chlorhexidine on group B streptococci
(1983) In European Journal of Obstetrics, Gynecology, and Reproductive Biology 16(3). p.157-165- Abstract
- Forty-three strains of group B streptococci (GBS) of types Ia, Ib, II and III were tested for susceptibility to chlorhexidine in concentrations ranging from 256 to 0.25 mg/l using the agar and tube dilution methods. The strains showed minimum inhibitory concentration (MIC) values ranging from 0.5 to 1 mg/l. Serum added to the test medium (50%) increased the MIC values to 4-8 mg/l, while amniotic fluid (50%) had almost no effect, increasing the values to 1-2 mg/l. The minimum bactericidal concentration (MBC) ranged from 1 to 5 mg/l. The killing kinetics were related to the concentration of chlorhexidine and the length of exposure. For example, at a concentration of 63 mg/l, 7 h were required for a bactericidal effect in broth, as compared... (More)
- Forty-three strains of group B streptococci (GBS) of types Ia, Ib, II and III were tested for susceptibility to chlorhexidine in concentrations ranging from 256 to 0.25 mg/l using the agar and tube dilution methods. The strains showed minimum inhibitory concentration (MIC) values ranging from 0.5 to 1 mg/l. Serum added to the test medium (50%) increased the MIC values to 4-8 mg/l, while amniotic fluid (50%) had almost no effect, increasing the values to 1-2 mg/l. The minimum bactericidal concentration (MBC) ranged from 1 to 5 mg/l. The killing kinetics were related to the concentration of chlorhexidine and the length of exposure. For example, at a concentration of 63 mg/l, 7 h were required for a bactericidal effect in broth, as compared to 1 h at 500 mg/l chlorhexidine. 200 mg/l chlordexidine had no effect on the adherence of two GBS strains to vaginal epithelial cells, and no effect on the phagocytosis of GBS with mouse peritoneal macrophages. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1103144
- author
- Kvist Christensen, Karen ; Christensen, Poul ; Dykes, Anna-Karin LU ; Kahlmeter, Gunnar ; Kurl, Daya N and Linden, Viveka
- organization
- publishing date
- 1983
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- group B streptococci, chlorhexidine, minimal inhibitory concentration, minimal bactericidal concentration, adherence, phagocytosis
- in
- European Journal of Obstetrics, Gynecology, and Reproductive Biology
- volume
- 16
- issue
- 3
- pages
- 157 - 165
- publisher
- Elsevier
- external identifiers
-
- pmid:6363151
- scopus:0021075141
- ISSN
- 0301-2115
- DOI
- 10.1016/0028-2243(83)90095-3
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Nursing (Closed 2012) (013065000), Department of Obstetrics and Gynaecology (Lund) (013018000), Division of Medical Microbiology (013250400)
- id
- 75ee651c-3d2d-4b31-85e3-c2882b080582 (old id 1103144)
- date added to LUP
- 2016-04-01 12:24:16
- date last changed
- 2021-09-19 03:10:16
@article{75ee651c-3d2d-4b31-85e3-c2882b080582, abstract = {{Forty-three strains of group B streptococci (GBS) of types Ia, Ib, II and III were tested for susceptibility to chlorhexidine in concentrations ranging from 256 to 0.25 mg/l using the agar and tube dilution methods. The strains showed minimum inhibitory concentration (MIC) values ranging from 0.5 to 1 mg/l. Serum added to the test medium (50%) increased the MIC values to 4-8 mg/l, while amniotic fluid (50%) had almost no effect, increasing the values to 1-2 mg/l. The minimum bactericidal concentration (MBC) ranged from 1 to 5 mg/l. The killing kinetics were related to the concentration of chlorhexidine and the length of exposure. For example, at a concentration of 63 mg/l, 7 h were required for a bactericidal effect in broth, as compared to 1 h at 500 mg/l chlorhexidine. 200 mg/l chlordexidine had no effect on the adherence of two GBS strains to vaginal epithelial cells, and no effect on the phagocytosis of GBS with mouse peritoneal macrophages.}}, author = {{Kvist Christensen, Karen and Christensen, Poul and Dykes, Anna-Karin and Kahlmeter, Gunnar and Kurl, Daya N and Linden, Viveka}}, issn = {{0301-2115}}, keywords = {{group B streptococci; chlorhexidine; minimal inhibitory concentration; minimal bactericidal concentration; adherence; phagocytosis}}, language = {{eng}}, number = {{3}}, pages = {{157--165}}, publisher = {{Elsevier}}, series = {{European Journal of Obstetrics, Gynecology, and Reproductive Biology}}, title = {{Chlorhexidine for prevention of neonatal colonization with group B streptococci. I. In vitro effect of chlorhexidine on group B streptococci}}, url = {{http://dx.doi.org/10.1016/0028-2243(83)90095-3}}, doi = {{10.1016/0028-2243(83)90095-3}}, volume = {{16}}, year = {{1983}}, }