Concurrent Isolation of Lymphocytes and Granulocytes Using Prefocused Free Flow Acoustophoresis
Grenvall, Carl LU ; Magnusson, Cecilia LU ; Lilja, Hans LU
and Laurell, Thomas
LU
(2015)
In Analytical Chemistry
87(11).
p.5596-5604
- Abstract
- Microchip-based free flow acoustophoresis (FFA) in combination with two-dimensional cell prefocusing enables concurrent multiple target outlet fractionation of leukocytes into subpopulations (lymphocytes, monocytes, and granulocytes); we report on this method here. We also observed significantly increased accuracy in size-based fractionation of microbeads as compared to previously presented FFA multiple outlet systems. Fluorescence microscopy illustrates the importance of two-dimensional prefocusing where a sample mixture of 3, 7, and 10 mu m beads are separated into well-confined particle streams and collected in their respective target outlets. Flow cytometry data for lymphocytes and granulocytes, respectively, in their corresponding... (More)
- Microchip-based free flow acoustophoresis (FFA) in combination with two-dimensional cell prefocusing enables concurrent multiple target outlet fractionation of leukocytes into subpopulations (lymphocytes, monocytes, and granulocytes); we report on this method here. We also observed significantly increased accuracy in size-based fractionation of microbeads as compared to previously presented FFA multiple outlet systems. Fluorescence microscopy illustrates the importance of two-dimensional prefocusing where a sample mixture of 3, 7, and 10 mu m beads are separated into well-confined particle streams and collected in their respective target outlets. Flow cytometry data for lymphocytes and granulocytes, respectively, in their corresponding outlets verify concurrent isolation of leukocyte subpopulations with high purity (95.2 +/- 0.6% and 98.5 +/- 0.7%) and high recovery (86.5 +/- 10.9% and 68.4 +/- 10.6%). A relatively low purity and high recovery of monocytes (25.2% +/- 5.4% and 83.1 +/- 4.3%) was obtained in the third target outlet. No subpopulation bias was observed. These data demonstrate an unprecedented separation of leukocyte subpopulations at flow rates of similar to 100 mu L/min and similar to 1 M cells/mL sample concentrations, not previously reported in acoustofluidic systems. Two-dimensional prefocusing FFA with multiple target outlets is a viable alternative to current methods for particle fractionation and cell isolation, requiring a minimum of sample preparation and lowering analysis time and cost. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7605061
- author
- Grenvall, Carl
LU
; Magnusson, Cecilia
LU
; Lilja, Hans
LU
and Laurell, Thomas
LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 87
- issue
- 11
- pages
- 5596 - 5604
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000355779500023
- scopus:84930635154
- pmid:25909882
- ISSN
- 1520-6882
- DOI
- 10.1021/acs.analchem.5b00370
- language
- English
- LU publication?
- yes
- id
- 5aecc78e-10a8-4a8d-a992-f32f2a699c72 (old id 7605061)
- date added to LUP
- 2016-04-01 10:03:34
- date last changed
- 2022-04-12 01:31:51
@article{5aecc78e-10a8-4a8d-a992-f32f2a699c72,
abstract = {{Microchip-based free flow acoustophoresis (FFA) in combination with two-dimensional cell prefocusing enables concurrent multiple target outlet fractionation of leukocytes into subpopulations (lymphocytes, monocytes, and granulocytes); we report on this method here. We also observed significantly increased accuracy in size-based fractionation of microbeads as compared to previously presented FFA multiple outlet systems. Fluorescence microscopy illustrates the importance of two-dimensional prefocusing where a sample mixture of 3, 7, and 10 mu m beads are separated into well-confined particle streams and collected in their respective target outlets. Flow cytometry data for lymphocytes and granulocytes, respectively, in their corresponding outlets verify concurrent isolation of leukocyte subpopulations with high purity (95.2 +/- 0.6% and 98.5 +/- 0.7%) and high recovery (86.5 +/- 10.9% and 68.4 +/- 10.6%). A relatively low purity and high recovery of monocytes (25.2% +/- 5.4% and 83.1 +/- 4.3%) was obtained in the third target outlet. No subpopulation bias was observed. These data demonstrate an unprecedented separation of leukocyte subpopulations at flow rates of similar to 100 mu L/min and similar to 1 M cells/mL sample concentrations, not previously reported in acoustofluidic systems. Two-dimensional prefocusing FFA with multiple target outlets is a viable alternative to current methods for particle fractionation and cell isolation, requiring a minimum of sample preparation and lowering analysis time and cost.}},
author = {{Grenvall, Carl and Magnusson, Cecilia and Lilja, Hans and Laurell, Thomas}},
issn = {{1520-6882}},
language = {{eng}},
number = {{11}},
pages = {{5596--5604}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Analytical Chemistry}},
title = {{Concurrent Isolation of Lymphocytes and Granulocytes Using Prefocused Free Flow Acoustophoresis}},
url = {{http://dx.doi.org/10.1021/acs.analchem.5b00370}},
doi = {{10.1021/acs.analchem.5b00370}},
volume = {{87}},
year = {{2015}},
}