Rapid in vitro and in vivo Evaluation of Antimicrobial Formulations Using Bioluminescent Pathogenic Bacteria
(2022) In Bio-protocol 12(2).- Abstract
Basic and translational research needs rapid methods to test antimicrobial formulations. Bioluminescent bacteria and advanced imaging systems capable of acquiring bioluminescence enable us to quickly and longitudinally evaluate the efficacy of antimicrobials. Conventional approaches, such as radial diffusion and viable count assays, are time-consuming and do not allow for longitudinal analysis. Bioluminescence imaging is sensitive and gives vital spatial and temporal information on the infection status in the body. Here, using bioluminescent Pseudomonas aeruginosa, we describe an in vitro and an in vivo approach to rapidly evaluate the antimicrobial efficacy of the host-defense peptide TCP-25.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7608b964-48ee-4c5c-b814-fa499c1cafbe
- author
- Schmidtchen, Artur LU and Puthia, Manoj LU
- organization
- publishing date
- 2022-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Antimicrobials, Bioluminescent, In vivo imaging, In vivo models, Infection
- in
- Bio-protocol
- volume
- 12
- issue
- 2
- article number
- e4302
- publisher
- Bio-protocol LLC
- external identifiers
-
- pmid:35127992
- scopus:85123407176
- ISSN
- 2331-8325
- DOI
- 10.21769/BioProtoc.4302
- language
- English
- LU publication?
- yes
- id
- 7608b964-48ee-4c5c-b814-fa499c1cafbe
- date added to LUP
- 2022-04-11 11:44:14
- date last changed
- 2024-06-01 09:36:53
@article{7608b964-48ee-4c5c-b814-fa499c1cafbe, abstract = {{<p>Basic and translational research needs rapid methods to test antimicrobial formulations. Bioluminescent bacteria and advanced imaging systems capable of acquiring bioluminescence enable us to quickly and longitudinally evaluate the efficacy of antimicrobials. Conventional approaches, such as radial diffusion and viable count assays, are time-consuming and do not allow for longitudinal analysis. Bioluminescence imaging is sensitive and gives vital spatial and temporal information on the infection status in the body. Here, using bioluminescent Pseudomonas aeruginosa, we describe an in vitro and an in vivo approach to rapidly evaluate the antimicrobial efficacy of the host-defense peptide TCP-25.</p>}}, author = {{Schmidtchen, Artur and Puthia, Manoj}}, issn = {{2331-8325}}, keywords = {{Antimicrobials; Bioluminescent; In vivo imaging; In vivo models; Infection}}, language = {{eng}}, number = {{2}}, publisher = {{Bio-protocol LLC}}, series = {{Bio-protocol}}, title = {{Rapid in vitro and in vivo Evaluation of Antimicrobial Formulations Using Bioluminescent Pathogenic Bacteria}}, url = {{http://dx.doi.org/10.21769/BioProtoc.4302}}, doi = {{10.21769/BioProtoc.4302}}, volume = {{12}}, year = {{2022}}, }