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Rapid in vitro and in vivo Evaluation of Antimicrobial Formulations Using Bioluminescent Pathogenic Bacteria

Schmidtchen, Artur LU and Puthia, Manoj LU (2022) In Bio-protocol 12(2).
Abstract

Basic and translational research needs rapid methods to test antimicrobial formulations. Bioluminescent bacteria and advanced imaging systems capable of acquiring bioluminescence enable us to quickly and longitudinally evaluate the efficacy of antimicrobials. Conventional approaches, such as radial diffusion and viable count assays, are time-consuming and do not allow for longitudinal analysis. Bioluminescence imaging is sensitive and gives vital spatial and temporal information on the infection status in the body. Here, using bioluminescent Pseudomonas aeruginosa, we describe an in vitro and an in vivo approach to rapidly evaluate the antimicrobial efficacy of the host-defense peptide TCP-25.

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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antimicrobials, Bioluminescent, In vivo imaging, In vivo models, Infection
in
Bio-protocol
volume
12
issue
2
article number
e4302
publisher
Bio-protocol LLC
external identifiers
  • pmid:35127992
  • scopus:85123407176
ISSN
2331-8325
DOI
10.21769/BioProtoc.4302
language
English
LU publication?
yes
id
7608b964-48ee-4c5c-b814-fa499c1cafbe
date added to LUP
2022-04-11 11:44:14
date last changed
2024-06-01 09:36:53
@article{7608b964-48ee-4c5c-b814-fa499c1cafbe,
  abstract     = {{<p>Basic and translational research needs rapid methods to test antimicrobial formulations. Bioluminescent bacteria and advanced imaging systems capable of acquiring bioluminescence enable us to quickly and longitudinally evaluate the efficacy of antimicrobials. Conventional approaches, such as radial diffusion and viable count assays, are time-consuming and do not allow for longitudinal analysis. Bioluminescence imaging is sensitive and gives vital spatial and temporal information on the infection status in the body. Here, using bioluminescent Pseudomonas aeruginosa, we describe an in vitro and an in vivo approach to rapidly evaluate the antimicrobial efficacy of the host-defense peptide TCP-25.</p>}},
  author       = {{Schmidtchen, Artur and Puthia, Manoj}},
  issn         = {{2331-8325}},
  keywords     = {{Antimicrobials; Bioluminescent; In vivo imaging; In vivo models; Infection}},
  language     = {{eng}},
  number       = {{2}},
  publisher    = {{Bio-protocol LLC}},
  series       = {{Bio-protocol}},
  title        = {{Rapid in vitro and in vivo Evaluation of Antimicrobial Formulations Using Bioluminescent Pathogenic Bacteria}},
  url          = {{http://dx.doi.org/10.21769/BioProtoc.4302}},
  doi          = {{10.21769/BioProtoc.4302}},
  volume       = {{12}},
  year         = {{2022}},
}