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Integrated protein microchip assay with dual fluorescent- and MALDI read-out

Finnskog, David LU ; Ressine, Anton LU ; Laurell, Thomas LU and Marko-Varga, György LU (2004) In Journal of Proteome Research 3(5). p.988-994
Abstract
A pore chip protein array (PCPA) concept based on a dual readout configuration, fluorescence imaging, and MALDI-TOF MS has been developed. Highly packed, (>4000 Spots/cm(2)), antibody arrays were dispensed on the porous chip by using a piezo-electric microdispenser. Sandwich assay was made after blocking by addition of a secondary antibody either lgG-FITC-labeled or anti-Ang II. The antigen in the first system was a large protein (lgG), and in the other system, a FITC marked peptide Angiotensin II (Ang II) was used. Ang II antibodies showed specificity for Ang II, while the Ang I antibodies showed binding properties for Ang I, II, and Renin. Fluorescence and MALDI TOF MS read-out was made for lgG and Ang II. A major advantage of the... (More)
A pore chip protein array (PCPA) concept based on a dual readout configuration, fluorescence imaging, and MALDI-TOF MS has been developed. Highly packed, (>4000 Spots/cm(2)), antibody arrays were dispensed on the porous chip by using a piezo-electric microdispenser. Sandwich assay was made after blocking by addition of a secondary antibody either lgG-FITC-labeled or anti-Ang II. The antigen in the first system was a large protein (lgG), and in the other system, a FITC marked peptide Angiotensin II (Ang II) was used. Ang II antibodies showed specificity for Ang II, while the Ang I antibodies showed binding properties for Ang I, II, and Renin. Fluorescence and MALDI TOF MS read-out was made for lgG and Ang II. A major advantage of the dual read-out PCPA approach is that both affinity binding and mass identity are derived. Detection limits for Ang II on the chip is as low as 500 zmol (Ang II). (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
matrix-assisted laser desorption ionization time-of-flight
in
Journal of Proteome Research
volume
3
issue
5
pages
988 - 994
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000224693800009
  • scopus:7044237454
ISSN
1535-3893
DOI
10.1021/pr0499287
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biomedical Engineering (011200011), Analytical Chemistry (S/LTH) (011001004)
id
760e48d8-3724-4cf9-8aa5-9995b4965db5 (old id 138344)
date added to LUP
2016-04-01 11:41:23
date last changed
2022-02-25 19:56:54
@article{760e48d8-3724-4cf9-8aa5-9995b4965db5,
  abstract     = {{A pore chip protein array (PCPA) concept based on a dual readout configuration, fluorescence imaging, and MALDI-TOF MS has been developed. Highly packed, (>4000 Spots/cm(2)), antibody arrays were dispensed on the porous chip by using a piezo-electric microdispenser. Sandwich assay was made after blocking by addition of a secondary antibody either lgG-FITC-labeled or anti-Ang II. The antigen in the first system was a large protein (lgG), and in the other system, a FITC marked peptide Angiotensin II (Ang II) was used. Ang II antibodies showed specificity for Ang II, while the Ang I antibodies showed binding properties for Ang I, II, and Renin. Fluorescence and MALDI TOF MS read-out was made for lgG and Ang II. A major advantage of the dual read-out PCPA approach is that both affinity binding and mass identity are derived. Detection limits for Ang II on the chip is as low as 500 zmol (Ang II).}},
  author       = {{Finnskog, David and Ressine, Anton and Laurell, Thomas and Marko-Varga, György}},
  issn         = {{1535-3893}},
  keywords     = {{matrix-assisted laser desorption ionization time-of-flight}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{988--994}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Journal of Proteome Research}},
  title        = {{Integrated protein microchip assay with dual fluorescent- and MALDI read-out}},
  url          = {{http://dx.doi.org/10.1021/pr0499287}},
  doi          = {{10.1021/pr0499287}},
  volume       = {{3}},
  year         = {{2004}},
}