Boosting Membrane Interactions and Antimicrobial Effects of Photocatalytic Titanium Dioxide Nanoparticles by Peptide Coating
(2024) In Small 20(30).- Abstract
Photocatalytic nanoparticles offer antimicrobial effects under illumination due to the formation of reactive oxygen species (ROS), capable of degrading bacterial membranes. ROS may, however, also degrade human cell membranes and trigger toxicity. Since antimicrobial peptides (AMPs) may display excellent selectivity between human cells and bacteria, these may offer opportunities to effectively “target” nanoparticles to bacterial membranes for increased selectivity. Investigating this, photocatalytic TiO2 nanoparticles (NPs) are coated with the AMP LL-37, and ROS generation is found by C11-BODIPY to be essentially unaffected after AMP coating. Furthermore, peptide-coated TiO2 NPs retain their positive... (More)
Photocatalytic nanoparticles offer antimicrobial effects under illumination due to the formation of reactive oxygen species (ROS), capable of degrading bacterial membranes. ROS may, however, also degrade human cell membranes and trigger toxicity. Since antimicrobial peptides (AMPs) may display excellent selectivity between human cells and bacteria, these may offer opportunities to effectively “target” nanoparticles to bacterial membranes for increased selectivity. Investigating this, photocatalytic TiO2 nanoparticles (NPs) are coated with the AMP LL-37, and ROS generation is found by C11-BODIPY to be essentially unaffected after AMP coating. Furthermore, peptide-coated TiO2 NPs retain their positive ζ-potential also after 1–2 h of UV illumination, showing peptide degradation to be sufficiently limited to allow peptide-mediated targeting. In line with this, quartz crystal microbalance measurements show peptide coating to promote membrane binding of TiO2 NPs, particularly so for bacteria-like anionic and cholesterol-void membranes. As a result, membrane degradation during illumination is strongly promoted for such membranes, but not so for mammalian-like membranes. The mechanisms of these effects are elucidated by neutron reflectometry. Analogously, LL-37 coating promoted membrane rupture by TiO2 NPs for Gram-negative and Gram-positive bacteria, but not for human monocytes. These findings demonstrate that AMP coating may selectively boost the antimicrobial effects of photocatalytic NPs.
(Less)
- author
- Caselli, Lucrezia LU ; Parra-Ortiz, Elisa ; Micciulla, Samantha ; Skoda, Maximilian W.A. ; Häffner, Sara Malekkhaiat ; Nielsen, Emilie Marie ; van der Plas, Mariena J.A. and Malmsten, Martin LU
- organization
- publishing date
- 2024-07
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Antimicrobial peptides, lipid membranes, lipid oxidation, LL-37, photocatalysis, TiO
- in
- Small
- volume
- 20
- issue
- 30
- article number
- 2309496
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:38402437
- scopus:85186268019
- ISSN
- 1613-6810
- DOI
- 10.1002/smll.202309496
- language
- English
- LU publication?
- yes
- id
- 761708a2-4464-4cff-a7d5-551a34d4c98c
- date added to LUP
- 2025-01-09 12:12:28
- date last changed
- 2025-07-11 16:55:05
@article{761708a2-4464-4cff-a7d5-551a34d4c98c,
abstract = {{<p>Photocatalytic nanoparticles offer antimicrobial effects under illumination due to the formation of reactive oxygen species (ROS), capable of degrading bacterial membranes. ROS may, however, also degrade human cell membranes and trigger toxicity. Since antimicrobial peptides (AMPs) may display excellent selectivity between human cells and bacteria, these may offer opportunities to effectively “target” nanoparticles to bacterial membranes for increased selectivity. Investigating this, photocatalytic TiO<sub>2</sub> nanoparticles (NPs) are coated with the AMP LL-37, and ROS generation is found by C<sub>11</sub>-BODIPY to be essentially unaffected after AMP coating. Furthermore, peptide-coated TiO<sub>2</sub> NPs retain their positive ζ-potential also after 1–2 h of UV illumination, showing peptide degradation to be sufficiently limited to allow peptide-mediated targeting. In line with this, quartz crystal microbalance measurements show peptide coating to promote membrane binding of TiO<sub>2</sub> NPs, particularly so for bacteria-like anionic and cholesterol-void membranes. As a result, membrane degradation during illumination is strongly promoted for such membranes, but not so for mammalian-like membranes. The mechanisms of these effects are elucidated by neutron reflectometry. Analogously, LL-37 coating promoted membrane rupture by TiO<sub>2</sub> NPs for Gram-negative and Gram-positive bacteria, but not for human monocytes. These findings demonstrate that AMP coating may selectively boost the antimicrobial effects of photocatalytic NPs.</p>}},
author = {{Caselli, Lucrezia and Parra-Ortiz, Elisa and Micciulla, Samantha and Skoda, Maximilian W.A. and Häffner, Sara Malekkhaiat and Nielsen, Emilie Marie and van der Plas, Mariena J.A. and Malmsten, Martin}},
issn = {{1613-6810}},
keywords = {{Antimicrobial peptides; lipid membranes; lipid oxidation; LL-37; photocatalysis; TiO}},
language = {{eng}},
number = {{30}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Small}},
title = {{Boosting Membrane Interactions and Antimicrobial Effects of Photocatalytic Titanium Dioxide Nanoparticles by Peptide Coating}},
url = {{http://dx.doi.org/10.1002/smll.202309496}},
doi = {{10.1002/smll.202309496}},
volume = {{20}},
year = {{2024}},
}