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Symbiotic fungi that are essential for plant nutrient uptake investigated with NMP

Pallon, Jan LU ; Wallander, Håkan LU ; Hammer, Edith LU ; Arteaga, Natalia LU ; Auzelyte, Vaida LU ; Elfman, Mikael LU ; Kristiansson, Per LU ; Nilsson, Charlotta LU ; Olsson, Pål Axel LU and Wegdén, Marie LU (2007) In Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms 260(1). p.149-152
Abstract
The nuclear microprobe (NMP) technique using PIXE for elemental analysis and STIM on/off axis for parallel mass density normalization has proven successful to investigate possible interactions between minerals and ectomycorrhizal (EM) mycelia that form symbiotic associations with forest trees. The ability for the EM to make elements biologically available from minerals and soil were compared in field studies and in laboratory experiments, and molecular analysis (PCR-RFLP) was used to identify ectomycorrhizal species from the field samplings. EM rhizomorphs associated with apatite in laboratory systems and in mesh bags incubated in forest ecosystems contained larger amounts of Ca than similar rhizomorphs connected to acid-washed sand. EM... (More)
The nuclear microprobe (NMP) technique using PIXE for elemental analysis and STIM on/off axis for parallel mass density normalization has proven successful to investigate possible interactions between minerals and ectomycorrhizal (EM) mycelia that form symbiotic associations with forest trees. The ability for the EM to make elements biologically available from minerals and soil were compared in field studies and in laboratory experiments, and molecular analysis (PCR-RFLP) was used to identify ectomycorrhizal species from the field samplings. EM rhizomorphs associated with apatite in laboratory systems and in mesh bags incubated in forest ecosystems contained larger amounts of Ca than similar rhizomorphs connected to acid-washed sand. EM mycelium produced in mesh bags had a capacity to mobilize P from apatite-amended sand and a high concentration of K in some rhizomorphs suggests that these fungi are good accumulators of K and may have a significant role in transporting K to trees.



Spores formed by arbuscular mycorrhizal (AM) fungi in laboratory cultures were compared with spores formed in saline soils in Tunisia in Northern Africa. We found lower concentrations of P and higher concentrations of Cl in the spores collected from the field than in the spores collected from laboratory cultures. For the case of laboratory cultures, the distribution of e.g. P and K was found to be clearly correlated. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Rhizomorphs, Arbuscular mycorrhiza, PIXE, Spores, Ectomycorrhizal, STIM
in
Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms
volume
260
issue
1
pages
149 - 152
publisher
Elsevier
external identifiers
  • wos:000248264900029
  • scopus:34249883545
ISSN
0168-583X
DOI
10.1016/j.nimb.2007.02.018
language
English
LU publication?
yes
id
f466a23a-4852-45ca-a3e8-6d665335f65a (old id 764155)
date added to LUP
2007-12-17 17:59:04
date last changed
2017-11-19 04:09:25
@article{f466a23a-4852-45ca-a3e8-6d665335f65a,
  abstract     = {The nuclear microprobe (NMP) technique using PIXE for elemental analysis and STIM on/off axis for parallel mass density normalization has proven successful to investigate possible interactions between minerals and ectomycorrhizal (EM) mycelia that form symbiotic associations with forest trees. The ability for the EM to make elements biologically available from minerals and soil were compared in field studies and in laboratory experiments, and molecular analysis (PCR-RFLP) was used to identify ectomycorrhizal species from the field samplings. EM rhizomorphs associated with apatite in laboratory systems and in mesh bags incubated in forest ecosystems contained larger amounts of Ca than similar rhizomorphs connected to acid-washed sand. EM mycelium produced in mesh bags had a capacity to mobilize P from apatite-amended sand and a high concentration of K in some rhizomorphs suggests that these fungi are good accumulators of K and may have a significant role in transporting K to trees.<br/><br>
<br/><br>
Spores formed by arbuscular mycorrhizal (AM) fungi in laboratory cultures were compared with spores formed in saline soils in Tunisia in Northern Africa. We found lower concentrations of P and higher concentrations of Cl in the spores collected from the field than in the spores collected from laboratory cultures. For the case of laboratory cultures, the distribution of e.g. P and K was found to be clearly correlated.},
  author       = {Pallon, Jan and Wallander, Håkan and Hammer, Edith and Arteaga, Natalia and Auzelyte, Vaida and Elfman, Mikael and Kristiansson, Per and Nilsson, Charlotta and Olsson, Pål Axel and Wegdén, Marie},
  issn         = {0168-583X},
  keyword      = {Rhizomorphs,Arbuscular mycorrhiza,PIXE,Spores,Ectomycorrhizal,STIM},
  language     = {eng},
  number       = {1},
  pages        = {149--152},
  publisher    = {Elsevier},
  series       = {Nuclear Instruments & Methods in Physics Research. Section B: Beam Interactions with Materials and Atoms},
  title        = {Symbiotic fungi that are essential for plant nutrient uptake investigated with NMP},
  url          = {http://dx.doi.org/10.1016/j.nimb.2007.02.018},
  volume       = {260},
  year         = {2007},
}