Monitoring of brain interstitial total tau and beta amyloid proteins by microdialysis in patients with traumatic brain injury
(2009) In Journal of Neurosurgery 110(6). p.37-1227- Abstract
OBJECT: Damage to axons contributes to postinjury disabilities and is commonly observed following traumatic brain injury (TBI). Traumatic brain injury is an important environmental risk factor for the development of Alzheimer disease (AD). In the present feasibility study, the aim was to use intracerebral microdialysis catheters with a high molecular cutoff membrane (100 kD) to harvest interstitial total tau (T-tau) and amyloid beta 1-42 (Abeta42) proteins, which are important biomarkers for axonal injury and for AD, following moderate-to-severe TBI.
METHODS: Eight patients (5 men and 3 women) were included in the study; 5 of the patients had a focal/mixed TBI and 3 had a diffuse axonal injury (DAI). Following the bedside analysis... (More)
OBJECT: Damage to axons contributes to postinjury disabilities and is commonly observed following traumatic brain injury (TBI). Traumatic brain injury is an important environmental risk factor for the development of Alzheimer disease (AD). In the present feasibility study, the aim was to use intracerebral microdialysis catheters with a high molecular cutoff membrane (100 kD) to harvest interstitial total tau (T-tau) and amyloid beta 1-42 (Abeta42) proteins, which are important biomarkers for axonal injury and for AD, following moderate-to-severe TBI.
METHODS: Eight patients (5 men and 3 women) were included in the study; 5 of the patients had a focal/mixed TBI and 3 had a diffuse axonal injury (DAI). Following the bedside analysis of the routinely measured energy metabolic markers (that is, glucose, lactate/pyruvate ratio, glycerol, and glutamate), the remaining dialysate was pooled and two 12-hour samples per day were used to analyze T-tau and Abeta42 by enzyme-linked immunosorbent assay from Day 1 up to 8 days postinjury.
RESULTS: The results show high levels of interstitial T-tau and Abeta42 postinjury. Patients with a predominantly focal lesion had higher interstitial T-tau levels than in the DAI group from Days 1 to 3 postinjury (p < 0.05). In contrast, patients with DAI had consistently higher Abeta42 levels when compared with patients with focal injury.
CONCLUSIONS: These results suggest that monitoring of interstitial T-tau and Abeta42 by using microdialysis may be an important tool when evaluating the presence and role of axonal injury following TBI.
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- author
- Marklund, Niklas LU ; Blennow, Kaj LU ; Zetterberg, Henrik LU ; Ronne-Engström, Elisabeth ; Enblad, Per and Hillered, Lars
- publishing date
- 2009-06
- type
- Contribution to journal
- publication status
- published
- keywords
- Adolescent, Adult, Aged, Amyloid beta-Peptides, Biomarkers, Cohort Studies, Diffuse Axonal Injury, Female, Humans, Male, Microdialysis, Middle Aged, Peptide Fragments, Pilot Projects, Predictive Value of Tests, Risk Factors, Young Adult, tau Proteins, Case Reports, Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't
- in
- Journal of Neurosurgery
- volume
- 110
- issue
- 6
- pages
- 37 - 1227
- publisher
- American Association of Neurosurgeons
- external identifiers
-
- pmid:19216653
- scopus:67650810148
- ISSN
- 0022-3085
- DOI
- 10.3171/2008.9.JNS08584
- language
- English
- LU publication?
- no
- id
- 76841ecc-76a9-40b1-b186-7c848f7db76f
- date added to LUP
- 2018-03-03 14:51:22
- date last changed
- 2024-06-24 10:40:44
@article{76841ecc-76a9-40b1-b186-7c848f7db76f, abstract = {{<p>OBJECT: Damage to axons contributes to postinjury disabilities and is commonly observed following traumatic brain injury (TBI). Traumatic brain injury is an important environmental risk factor for the development of Alzheimer disease (AD). In the present feasibility study, the aim was to use intracerebral microdialysis catheters with a high molecular cutoff membrane (100 kD) to harvest interstitial total tau (T-tau) and amyloid beta 1-42 (Abeta42) proteins, which are important biomarkers for axonal injury and for AD, following moderate-to-severe TBI.</p><p>METHODS: Eight patients (5 men and 3 women) were included in the study; 5 of the patients had a focal/mixed TBI and 3 had a diffuse axonal injury (DAI). Following the bedside analysis of the routinely measured energy metabolic markers (that is, glucose, lactate/pyruvate ratio, glycerol, and glutamate), the remaining dialysate was pooled and two 12-hour samples per day were used to analyze T-tau and Abeta42 by enzyme-linked immunosorbent assay from Day 1 up to 8 days postinjury.</p><p>RESULTS: The results show high levels of interstitial T-tau and Abeta42 postinjury. Patients with a predominantly focal lesion had higher interstitial T-tau levels than in the DAI group from Days 1 to 3 postinjury (p < 0.05). In contrast, patients with DAI had consistently higher Abeta42 levels when compared with patients with focal injury.</p><p>CONCLUSIONS: These results suggest that monitoring of interstitial T-tau and Abeta42 by using microdialysis may be an important tool when evaluating the presence and role of axonal injury following TBI.</p>}}, author = {{Marklund, Niklas and Blennow, Kaj and Zetterberg, Henrik and Ronne-Engström, Elisabeth and Enblad, Per and Hillered, Lars}}, issn = {{0022-3085}}, keywords = {{Adolescent; Adult; Aged; Amyloid beta-Peptides; Biomarkers; Cohort Studies; Diffuse Axonal Injury; Female; Humans; Male; Microdialysis; Middle Aged; Peptide Fragments; Pilot Projects; Predictive Value of Tests; Risk Factors; Young Adult; tau Proteins; Case Reports; Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't}}, language = {{eng}}, number = {{6}}, pages = {{37--1227}}, publisher = {{American Association of Neurosurgeons}}, series = {{Journal of Neurosurgery}}, title = {{Monitoring of brain interstitial total tau and beta amyloid proteins by microdialysis in patients with traumatic brain injury}}, url = {{http://dx.doi.org/10.3171/2008.9.JNS08584}}, doi = {{10.3171/2008.9.JNS08584}}, volume = {{110}}, year = {{2009}}, }