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Monitoring of brain interstitial total tau and beta amyloid proteins by microdialysis in patients with traumatic brain injury

Marklund, Niklas LU ; Blennow, Kaj LU ; Zetterberg, Henrik LU ; Ronne-Engström, Elisabeth; Enblad, Per and Hillered, Lars (2009) In Journal of Neurosurgery 110(6). p.37-1227
Abstract

OBJECT: Damage to axons contributes to postinjury disabilities and is commonly observed following traumatic brain injury (TBI). Traumatic brain injury is an important environmental risk factor for the development of Alzheimer disease (AD). In the present feasibility study, the aim was to use intracerebral microdialysis catheters with a high molecular cutoff membrane (100 kD) to harvest interstitial total tau (T-tau) and amyloid beta 1-42 (Abeta42) proteins, which are important biomarkers for axonal injury and for AD, following moderate-to-severe TBI.

METHODS: Eight patients (5 men and 3 women) were included in the study; 5 of the patients had a focal/mixed TBI and 3 had a diffuse axonal injury (DAI). Following the bedside analysis... (More)

OBJECT: Damage to axons contributes to postinjury disabilities and is commonly observed following traumatic brain injury (TBI). Traumatic brain injury is an important environmental risk factor for the development of Alzheimer disease (AD). In the present feasibility study, the aim was to use intracerebral microdialysis catheters with a high molecular cutoff membrane (100 kD) to harvest interstitial total tau (T-tau) and amyloid beta 1-42 (Abeta42) proteins, which are important biomarkers for axonal injury and for AD, following moderate-to-severe TBI.

METHODS: Eight patients (5 men and 3 women) were included in the study; 5 of the patients had a focal/mixed TBI and 3 had a diffuse axonal injury (DAI). Following the bedside analysis of the routinely measured energy metabolic markers (that is, glucose, lactate/pyruvate ratio, glycerol, and glutamate), the remaining dialysate was pooled and two 12-hour samples per day were used to analyze T-tau and Abeta42 by enzyme-linked immunosorbent assay from Day 1 up to 8 days postinjury.

RESULTS: The results show high levels of interstitial T-tau and Abeta42 postinjury. Patients with a predominantly focal lesion had higher interstitial T-tau levels than in the DAI group from Days 1 to 3 postinjury (p < 0.05). In contrast, patients with DAI had consistently higher Abeta42 levels when compared with patients with focal injury.

CONCLUSIONS: These results suggest that monitoring of interstitial T-tau and Abeta42 by using microdialysis may be an important tool when evaluating the presence and role of axonal injury following TBI.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Adolescent, Adult, Aged, Amyloid beta-Peptides, Biomarkers, Cohort Studies, Diffuse Axonal Injury, Female, Humans, Male, Microdialysis, Middle Aged, Peptide Fragments, Pilot Projects, Predictive Value of Tests, Risk Factors, Young Adult, tau Proteins, Case Reports, Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Neurosurgery
volume
110
issue
6
pages
37 - 1227
publisher
American Association of Neurosurgeons
external identifiers
  • scopus:67650810148
ISSN
0022-3085
DOI
10.3171/2008.9.JNS08584
language
English
LU publication?
no
id
76841ecc-76a9-40b1-b186-7c848f7db76f
date added to LUP
2018-03-03 14:51:22
date last changed
2018-10-14 04:46:27
@article{76841ecc-76a9-40b1-b186-7c848f7db76f,
  abstract     = {<p>OBJECT: Damage to axons contributes to postinjury disabilities and is commonly observed following traumatic brain injury (TBI). Traumatic brain injury is an important environmental risk factor for the development of Alzheimer disease (AD). In the present feasibility study, the aim was to use intracerebral microdialysis catheters with a high molecular cutoff membrane (100 kD) to harvest interstitial total tau (T-tau) and amyloid beta 1-42 (Abeta42) proteins, which are important biomarkers for axonal injury and for AD, following moderate-to-severe TBI.</p><p>METHODS: Eight patients (5 men and 3 women) were included in the study; 5 of the patients had a focal/mixed TBI and 3 had a diffuse axonal injury (DAI). Following the bedside analysis of the routinely measured energy metabolic markers (that is, glucose, lactate/pyruvate ratio, glycerol, and glutamate), the remaining dialysate was pooled and two 12-hour samples per day were used to analyze T-tau and Abeta42 by enzyme-linked immunosorbent assay from Day 1 up to 8 days postinjury.</p><p>RESULTS: The results show high levels of interstitial T-tau and Abeta42 postinjury. Patients with a predominantly focal lesion had higher interstitial T-tau levels than in the DAI group from Days 1 to 3 postinjury (p &lt; 0.05). In contrast, patients with DAI had consistently higher Abeta42 levels when compared with patients with focal injury.</p><p>CONCLUSIONS: These results suggest that monitoring of interstitial T-tau and Abeta42 by using microdialysis may be an important tool when evaluating the presence and role of axonal injury following TBI.</p>},
  author       = {Marklund, Niklas and Blennow, Kaj and Zetterberg, Henrik and Ronne-Engström, Elisabeth and Enblad, Per and Hillered, Lars},
  issn         = {0022-3085},
  keyword      = {Adolescent,Adult,Aged,Amyloid beta-Peptides,Biomarkers,Cohort Studies,Diffuse Axonal Injury,Female,Humans,Male,Microdialysis,Middle Aged,Peptide Fragments,Pilot Projects,Predictive Value of Tests,Risk Factors,Young Adult,tau Proteins,Case Reports,Clinical Trial,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {6},
  pages        = {37--1227},
  publisher    = {American Association of Neurosurgeons},
  series       = {Journal of Neurosurgery},
  title        = {Monitoring of brain interstitial total tau and beta amyloid proteins by microdialysis in patients with traumatic brain injury},
  url          = {http://dx.doi.org/10.3171/2008.9.JNS08584},
  volume       = {110},
  year         = {2009},
}