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Studies on the interaction between hyaluronan and a rat colon cancer cell line

Samuelsson, C LU and Gustafson, S (1998) In Glycoconjugate Journal 15(2). p.75-169
Abstract

Binding studies with 125I-Tyr labelled hyaluronan (HA) on a cultured rat colon cancer cell line were performed to characterize the association of HA to tumour cells in vitro. Results show a specific and saturable binding (Kd=1.36 nM) which indicates the presence of an HA binding receptor on the tumour cells. There is a specific constant increase of cell-associated HA over time, which indicates that HA is specifically taken up by the cells through endocytosis. The binding of 125I-Tyr labelled HA was more effectively inhibited by unlabelled HA of high MW in relation to low MW species of the polysaccharide indicating that the receptor binds HA of high MW with greater affinity than low MW species. In competition experiments, the HA-binding... (More)

Binding studies with 125I-Tyr labelled hyaluronan (HA) on a cultured rat colon cancer cell line were performed to characterize the association of HA to tumour cells in vitro. Results show a specific and saturable binding (Kd=1.36 nM) which indicates the presence of an HA binding receptor on the tumour cells. There is a specific constant increase of cell-associated HA over time, which indicates that HA is specifically taken up by the cells through endocytosis. The binding of 125I-Tyr labelled HA was more effectively inhibited by unlabelled HA of high MW in relation to low MW species of the polysaccharide indicating that the receptor binds HA of high MW with greater affinity than low MW species. In competition experiments, the HA-binding could not be inhibited by other polysaccharides such as chondroitin sulphate and heparin. Nor could ligands for scavenger receptors and antibodies directed towards ICAM-1, CD 44 and RHAMM (Receptor for HA Mediated Motility) significantly inhibit the association of HA to tumour cells.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Antibodies/pharmacology, Chondroitin Sulfates/pharmacology, Colonic Neoplasms/drug therapy, Dextran Sulfate/pharmacology, Endothelium/cytology, Extracellular Matrix Proteins/immunology, Hyaluronan Receptors/immunology, Hyaluronic Acid/metabolism, Intercellular Adhesion Molecule-1/immunology, Liver/cytology, Mice, Rats, Rats, Wistar, Tumor Cells, Cultured
in
Glycoconjugate Journal
volume
15
issue
2
pages
75 - 169
publisher
Springer
external identifiers
  • pmid:9557877
  • scopus:0031863536
ISSN
0282-0080
DOI
10.1023/A:1006920323095
language
English
LU publication?
no
id
76c34967-90c4-48e4-a00a-39f88bca12da
date added to LUP
2019-06-05 16:20:31
date last changed
2019-10-29 05:51:52
@article{76c34967-90c4-48e4-a00a-39f88bca12da,
  abstract     = {<p>Binding studies with 125I-Tyr labelled hyaluronan (HA) on a cultured rat colon cancer cell line were performed to characterize the association of HA to tumour cells in vitro. Results show a specific and saturable binding (Kd=1.36 nM) which indicates the presence of an HA binding receptor on the tumour cells. There is a specific constant increase of cell-associated HA over time, which indicates that HA is specifically taken up by the cells through endocytosis. The binding of 125I-Tyr labelled HA was more effectively inhibited by unlabelled HA of high MW in relation to low MW species of the polysaccharide indicating that the receptor binds HA of high MW with greater affinity than low MW species. In competition experiments, the HA-binding could not be inhibited by other polysaccharides such as chondroitin sulphate and heparin. Nor could ligands for scavenger receptors and antibodies directed towards ICAM-1, CD 44 and RHAMM (Receptor for HA Mediated Motility) significantly inhibit the association of HA to tumour cells.</p>},
  author       = {Samuelsson, C and Gustafson, S},
  issn         = {0282-0080},
  language     = {eng},
  number       = {2},
  pages        = {75--169},
  publisher    = {Springer},
  series       = {Glycoconjugate Journal},
  title        = {Studies on the interaction between hyaluronan and a rat colon cancer cell line},
  url          = {http://dx.doi.org/10.1023/A:1006920323095},
  doi          = {10.1023/A:1006920323095},
  volume       = {15},
  year         = {1998},
}