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Essential role of DivIVA in polar growth and morphogenesis in Streptomyces coelicolor A3(2)

Flärdh, Klas LU (2003) In Molecular Microbiology 49(6). p.1523-1536
Abstract
Streptomycetes grow by cell wall extension at hyphal

tips. The molecular basis for such polar growth in

prokaryotes is largely unknown. It is reported here

that DivIVA

SC

, the

Streptomyces coelicolor

homologue

of the

Bacillus subtilis

protein DivIVA, is essential

and directly involved in hyphal tip growth and

morphogenesis. A DivIVA

SC

-EGFP hybrid was distinctively

localized to hyphal tips and lateral branches.

Reduction of

divIVA

SC

expression to about 10% of the

normal level produced a phenotype strikingly similar

to that of many tip growth mutants... (More)
Streptomycetes grow by cell wall extension at hyphal

tips. The molecular basis for such polar growth in

prokaryotes is largely unknown. It is reported here

that DivIVA

SC

, the

Streptomyces coelicolor

homologue

of the

Bacillus subtilis

protein DivIVA, is essential

and directly involved in hyphal tip growth and

morphogenesis. A DivIVA

SC

-EGFP hybrid was distinctively

localized to hyphal tips and lateral branches.

Reduction of

divIVA

SC

expression to about 10% of the

normal level produced a phenotype strikingly similar

to that of many tip growth mutants in fungi, including

irregular curly hyphae and apical branching. Overexpression

of the gene dramatically perturbed determination

of cell shape at the growing tips. Furthermore,

staining of nascent peptidoglycan with a fluorescent

vancomycin conjugate revealed that induction of

overexpression in normal hyphae disturbed tip

growth, and gave rise to several new sites of cell wall

assembly, effectively causing hyperbranching. The

results show that DivIVA

SC

is a novel bacterial morphogene,

and it is localized at or very close to the

apical sites of peptidoglycan assembly in

Streptomyces

hyphae. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Microbiology
volume
49
issue
6
pages
1523 - 1536
publisher
Wiley-Blackwell
external identifiers
  • scopus:0141789744
ISSN
1365-2958
DOI
10.1046/j.1365-2958.2003.03660.x
language
English
LU publication?
no
id
1dd2911c-e59e-4a21-9117-fdcc9ba17984 (old id 770242)
date added to LUP
2007-12-18 16:33:26
date last changed
2017-06-25 03:40:38
@article{1dd2911c-e59e-4a21-9117-fdcc9ba17984,
  abstract     = {Streptomycetes grow by cell wall extension at hyphal<br/><br>
tips. The molecular basis for such polar growth in<br/><br>
prokaryotes is largely unknown. It is reported here<br/><br>
that DivIVA<br/><br>
SC<br/><br>
, the<br/><br>
Streptomyces coelicolor<br/><br>
homologue<br/><br>
of the<br/><br>
Bacillus subtilis<br/><br>
protein DivIVA, is essential<br/><br>
and directly involved in hyphal tip growth and<br/><br>
morphogenesis. A DivIVA<br/><br>
SC<br/><br>
-EGFP hybrid was distinctively<br/><br>
localized to hyphal tips and lateral branches.<br/><br>
Reduction of<br/><br>
divIVA<br/><br>
SC<br/><br>
expression to about 10% of the<br/><br>
normal level produced a phenotype strikingly similar<br/><br>
to that of many tip growth mutants in fungi, including<br/><br>
irregular curly hyphae and apical branching. Overexpression<br/><br>
of the gene dramatically perturbed determination<br/><br>
of cell shape at the growing tips. Furthermore,<br/><br>
staining of nascent peptidoglycan with a fluorescent<br/><br>
vancomycin conjugate revealed that induction of<br/><br>
overexpression in normal hyphae disturbed tip<br/><br>
growth, and gave rise to several new sites of cell wall<br/><br>
assembly, effectively causing hyperbranching. The<br/><br>
results show that DivIVA<br/><br>
SC<br/><br>
is a novel bacterial morphogene,<br/><br>
and it is localized at or very close to the<br/><br>
apical sites of peptidoglycan assembly in<br/><br>
Streptomyces<br/><br>
hyphae.},
  author       = {Flärdh, Klas},
  issn         = {1365-2958},
  language     = {eng},
  number       = {6},
  pages        = {1523--1536},
  publisher    = {Wiley-Blackwell},
  series       = {Molecular Microbiology},
  title        = {Essential role of DivIVA in polar growth and morphogenesis in Streptomyces coelicolor A3(2)},
  url          = {http://dx.doi.org/10.1046/j.1365-2958.2003.03660.x},
  volume       = {49},
  year         = {2003},
}