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Identification of the miRNA targetome in hippocampal neurons using RIP-seq.

Malmevik, Josephine LU ; Petri, Rebecca LU ; Klüssendorf, Thies LU ; Knauff, Pina LU ; Åkerblom, Malin LU ; Johansson, Jenny G LU ; Soneji, Shamit LU and Jakobsson, Johan LU (2015) In Scientific Reports 5.
Abstract
MicroRNAs (miRNAs) are key players in the regulation of neuronal processes by targeting a large network of target messenger RNAs (mRNAs). However, the identity and function of mRNAs targeted by miRNAs in specific cells of the brain are largely unknown. Here, we established an adeno-associated viral vector (AAV)-based neuron-specific Argonaute2:GFP-RNA immunoprecipitation followed by high-throughput sequencing to analyse the regulatory role of miRNAs in mouse hippocampal neurons. Using this approach, we identified more than two thousand miRNA targets in hippocampal neurons, regulating essential neuronal features such as cell signalling, transcription and axon guidance. Furthermore, we found that stable inhibition of the highly expressed... (More)
MicroRNAs (miRNAs) are key players in the regulation of neuronal processes by targeting a large network of target messenger RNAs (mRNAs). However, the identity and function of mRNAs targeted by miRNAs in specific cells of the brain are largely unknown. Here, we established an adeno-associated viral vector (AAV)-based neuron-specific Argonaute2:GFP-RNA immunoprecipitation followed by high-throughput sequencing to analyse the regulatory role of miRNAs in mouse hippocampal neurons. Using this approach, we identified more than two thousand miRNA targets in hippocampal neurons, regulating essential neuronal features such as cell signalling, transcription and axon guidance. Furthermore, we found that stable inhibition of the highly expressed miR-124 and miR-125 in hippocampal neurons led to significant but distinct changes in the AGO2 binding of target mRNAs, resulting in subsequent upregulation of numerous miRNA target genes. These findings greatly enhance our understanding of the miRNA targetome in hippocampal neurons. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
5
publisher
Nature Publishing Group
external identifiers
  • pmid:26219083
  • wos:000358583300001
  • scopus:84938271115
ISSN
2045-2322
DOI
10.1038/srep12609
language
English
LU publication?
yes
id
b06a12a6-75f1-457f-abc1-4cc8689b755c (old id 7703656)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26219083?dopt=Abstract
date added to LUP
2015-08-11 12:34:07
date last changed
2017-07-30 04:13:08
@article{b06a12a6-75f1-457f-abc1-4cc8689b755c,
  abstract     = {MicroRNAs (miRNAs) are key players in the regulation of neuronal processes by targeting a large network of target messenger RNAs (mRNAs). However, the identity and function of mRNAs targeted by miRNAs in specific cells of the brain are largely unknown. Here, we established an adeno-associated viral vector (AAV)-based neuron-specific Argonaute2:GFP-RNA immunoprecipitation followed by high-throughput sequencing to analyse the regulatory role of miRNAs in mouse hippocampal neurons. Using this approach, we identified more than two thousand miRNA targets in hippocampal neurons, regulating essential neuronal features such as cell signalling, transcription and axon guidance. Furthermore, we found that stable inhibition of the highly expressed miR-124 and miR-125 in hippocampal neurons led to significant but distinct changes in the AGO2 binding of target mRNAs, resulting in subsequent upregulation of numerous miRNA target genes. These findings greatly enhance our understanding of the miRNA targetome in hippocampal neurons.},
  articleno    = {12609},
  author       = {Malmevik, Josephine and Petri, Rebecca and Klüssendorf, Thies and Knauff, Pina and Åkerblom, Malin and Johansson, Jenny G and Soneji, Shamit and Jakobsson, Johan},
  issn         = {2045-2322},
  language     = {eng},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Identification of the miRNA targetome in hippocampal neurons using RIP-seq.},
  url          = {http://dx.doi.org/10.1038/srep12609},
  volume       = {5},
  year         = {2015},
}