Changing the Mechanical Unfolding Pathway of FnIII(10) by Tuning the Pulling Strength
Mitternacht, Simon LU ; Luccioli, Stefano ; Torcini, Alessandro ; Imparato, Alberto and Irbäck, Anders LU
(2009)
In Biophysical Journal
96(2).
p.429-441
- Abstract
- We investigate the mechanical unfolding of the tenth type III domain from fibronectin (FnIII(10)) both at constant force and at constant pulling velocity, by all-atom Monte Carlo simulations. We observe both apparent two-state unfolding and several unfolding pathways involving one of three major, mutually exclusive intermediate states. All three major intermediates lack two of seven native beta-strands, and share a quite similar extension. The unfolding behavior is found to depend strongly on the pulling conditions. In particular, we observe large variations in the relative frequencies of occurrence for the intermediates. At low constant force or low constant velocity, all three major intermediates occur with a significant frequency. At... (More)
- We investigate the mechanical unfolding of the tenth type III domain from fibronectin (FnIII(10)) both at constant force and at constant pulling velocity, by all-atom Monte Carlo simulations. We observe both apparent two-state unfolding and several unfolding pathways involving one of three major, mutually exclusive intermediate states. All three major intermediates lack two of seven native beta-strands, and share a quite similar extension. The unfolding behavior is found to depend strongly on the pulling conditions. In particular, we observe large variations in the relative frequencies of occurrence for the intermediates. At low constant force or low constant velocity, all three major intermediates occur with a significant frequency. At high constant force or high constant velocity, one of them, with the N- and C-terminal beta-strands detached, dominates over the other two. Using the extended Jarzynski equality, we also estimate the equilibrium free-energy landscape, calculated as a function of chain extension. The application of a constant pulling force leads to a free-energy profile with three major local minima. Two of these correspond to the native and fully unfolded states, respectively, whereas the third one can be associated with the major unfolding intermediates. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1425400
- author
- Mitternacht, Simon
LU
; Luccioli, Stefano
; Torcini, Alessandro
; Imparato, Alberto
and Irbäck, Anders
LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biophysical Journal
- volume
- 96
- issue
- 2
- pages
- 429 - 441
- publisher
- Cell Press
- external identifiers
-
- wos:000266377200015
- scopus:58849130229
- pmid:19167294
- ISSN
- 1542-0086
- DOI
- 10.1016/j.bpj.2008.09.043
- language
- English
- LU publication?
- yes
- id
- 77417d45-9c1f-455e-9383-dac9d9d954ac (old id 1425400)
- date added to LUP
- 2016-04-01 12:25:08
- date last changed
- 2024-04-09 12:32:18
@article{77417d45-9c1f-455e-9383-dac9d9d954ac,
abstract = {{We investigate the mechanical unfolding of the tenth type III domain from fibronectin (FnIII(10)) both at constant force and at constant pulling velocity, by all-atom Monte Carlo simulations. We observe both apparent two-state unfolding and several unfolding pathways involving one of three major, mutually exclusive intermediate states. All three major intermediates lack two of seven native beta-strands, and share a quite similar extension. The unfolding behavior is found to depend strongly on the pulling conditions. In particular, we observe large variations in the relative frequencies of occurrence for the intermediates. At low constant force or low constant velocity, all three major intermediates occur with a significant frequency. At high constant force or high constant velocity, one of them, with the N- and C-terminal beta-strands detached, dominates over the other two. Using the extended Jarzynski equality, we also estimate the equilibrium free-energy landscape, calculated as a function of chain extension. The application of a constant pulling force leads to a free-energy profile with three major local minima. Two of these correspond to the native and fully unfolded states, respectively, whereas the third one can be associated with the major unfolding intermediates.}},
author = {{Mitternacht, Simon and Luccioli, Stefano and Torcini, Alessandro and Imparato, Alberto and Irbäck, Anders}},
issn = {{1542-0086}},
language = {{eng}},
number = {{2}},
pages = {{429--441}},
publisher = {{Cell Press}},
series = {{Biophysical Journal}},
title = {{Changing the Mechanical Unfolding Pathway of FnIII(10) by Tuning the Pulling Strength}},
url = {{http://dx.doi.org/10.1016/j.bpj.2008.09.043}},
doi = {{10.1016/j.bpj.2008.09.043}},
volume = {{96}},
year = {{2009}},
}