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A novel feeding strategy for industrial fed-batch processes based on frequency content analysis

Johnsson, Ola LU ; Andersson, Jonas; Johnsson, Charlotta LU ; Lidén, Gunnar LU and Hägglund, Tore LU (2012) 15th European Congress on Biotechnology In New Biotechnology 29(Supplement). p.11-11
Abstract
Overflow metabolism, i.e. the production of metabolic by-products at a high glycolytic flux, is a recurring problem in fed-batch processes with many types of microorganisms. In the current study, a novel feeding strategy aimed at avoiding process failures due to overflow by-product formation was designed and implemented in a pilot-scale reactor (0.5 m3). The basic principle behind the strategy was to analyze the effects on the dissolved oxygen concentration by periodic variations in the inlet feed rate. The frequency spectrum of the dissolved oxygen signal was used to estimate the proximity of the system to the region where overflow metabolism occurs by examining the content in the relevant frequency range. A control variable based on the... (More)
Overflow metabolism, i.e. the production of metabolic by-products at a high glycolytic flux, is a recurring problem in fed-batch processes with many types of microorganisms. In the current study, a novel feeding strategy aimed at avoiding process failures due to overflow by-product formation was designed and implemented in a pilot-scale reactor (0.5 m3). The basic principle behind the strategy was to analyze the effects on the dissolved oxygen concentration by periodic variations in the inlet feed rate. The frequency spectrum of the dissolved oxygen signal was used to estimate the proximity of the system to the region where overflow metabolism occurs by examining the content in the relevant frequency range. A control variable based on the measured frequency content was subsequently used to control the feed rate. The only measurement required for this strategy is the dissolved oxygen level in the broth, for which robust, fast and precise probes are widely available in industrial fermentors today.



The strategy was successfully implemented in pilot-scale processes for industrial enzyme production using Bacillus licheniformis. It was shown possible to run the process close to the optimal feed rate, indicated by very low amounts of acetate (the overflow metabolite) in the broth. In comparison to a reference strategy the new control strategy resulted in over 10% higher biomass yields. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
New Biotechnology
volume
29
issue
Supplement
pages
1 pages
publisher
Elsevier
conference name
15th European Congress on Biotechnology
ISSN
1876-4347
DOI
10.1016/j.nbt.2012.08.022
language
English
LU publication?
yes
id
bfe52508-d8d1-4919-8181-e5e363ff0ff3 (old id 7762754)
date added to LUP
2013-08-17 12:02:56
date last changed
2017-02-16 08:48:22
@misc{bfe52508-d8d1-4919-8181-e5e363ff0ff3,
  abstract     = {Overflow metabolism, i.e. the production of metabolic by-products at a high glycolytic flux, is a recurring problem in fed-batch processes with many types of microorganisms. In the current study, a novel feeding strategy aimed at avoiding process failures due to overflow by-product formation was designed and implemented in a pilot-scale reactor (0.5 m3). The basic principle behind the strategy was to analyze the effects on the dissolved oxygen concentration by periodic variations in the inlet feed rate. The frequency spectrum of the dissolved oxygen signal was used to estimate the proximity of the system to the region where overflow metabolism occurs by examining the content in the relevant frequency range. A control variable based on the measured frequency content was subsequently used to control the feed rate. The only measurement required for this strategy is the dissolved oxygen level in the broth, for which robust, fast and precise probes are widely available in industrial fermentors today.<br/><br>
<br/><br>
The strategy was successfully implemented in pilot-scale processes for industrial enzyme production using Bacillus licheniformis. It was shown possible to run the process close to the optimal feed rate, indicated by very low amounts of acetate (the overflow metabolite) in the broth. In comparison to a reference strategy the new control strategy resulted in over 10% higher biomass yields.},
  author       = {Johnsson, Ola and Andersson, Jonas and Johnsson, Charlotta and Lidén, Gunnar and Hägglund, Tore},
  issn         = {1876-4347},
  language     = {eng},
  note         = {Conference Abstract},
  number       = {Supplement},
  pages        = {11--11},
  publisher    = {Elsevier},
  series       = {New Biotechnology},
  title        = {A novel feeding strategy for industrial fed-batch processes based on frequency content analysis},
  url          = {http://dx.doi.org/10.1016/j.nbt.2012.08.022},
  volume       = {29},
  year         = {2012},
}