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Faster Protocol for Endogenous Fatty Acid Esters of Hydroxy Fatty Acid (FAHFA) Measurements

Kolar, Matthew J. ; Nelson, Andrew T. ; Chang, Tina ; Ertunc, Meric Erikci ; Christy, Mitchell P. ; Ohlsson, Lena LU ; Härröd, Magnus ; Kahn, Barbara B. ; Siegel, Dionicio and Saghatelian, Alan (2018) In Analytical Chemistry 90(8). p.5358-5365
Abstract

Fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of endogenous lipids with antidiabetic and anti-inflammatory activities. Interest in these lipids is due to their unique biological activites and the observation that insulin-resistant people have lower palmitic acid esters of hydroxystearic acid (PAHSA) levels, suggesting that a FAHFA deficiency may contribute to metabolic disease. Rigorous testing of this hypothesis will require the measurement of many clinical samples; however, current analytical workflows are too slow to enable samples to be analyzed quickly. Here we describe the development of a significantly faster workflow to measure FAHFAs that optimizes the fractionation and chromatography of... (More)

Fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of endogenous lipids with antidiabetic and anti-inflammatory activities. Interest in these lipids is due to their unique biological activites and the observation that insulin-resistant people have lower palmitic acid esters of hydroxystearic acid (PAHSA) levels, suggesting that a FAHFA deficiency may contribute to metabolic disease. Rigorous testing of this hypothesis will require the measurement of many clinical samples; however, current analytical workflows are too slow to enable samples to be analyzed quickly. Here we describe the development of a significantly faster workflow to measure FAHFAs that optimizes the fractionation and chromatography of these lipids. We can measure FAHFAs in 30 min with this new protocol versus 90 min using the older protocol with comparable performance in regioisomer detection and quantitation. We also discovered through this optimization that oleic acid esters of hydroxystearic acids (OAHSAs), another family of FAHFAs, have a much lower background signal than PAHSAs, which makes them easier to measure. Our faster workflow was able to quantify changes in PAHSAs and OAHSAs in mouse tissues and human plasma, highlighting the potential of this protocol for basic and clinical applications.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
90
issue
8
pages
8 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:85045688156
  • pmid:29578702
ISSN
0003-2700
DOI
10.1021/acs.analchem.8b00503
language
English
LU publication?
yes
id
77c98984-bb77-46a6-bee2-af6827429c24
date added to LUP
2018-05-03 08:10:47
date last changed
2024-06-11 14:47:52
@article{77c98984-bb77-46a6-bee2-af6827429c24,
  abstract     = {{<p>Fatty acid esters of hydroxy fatty acids (FAHFAs) are a recently discovered class of endogenous lipids with antidiabetic and anti-inflammatory activities. Interest in these lipids is due to their unique biological activites and the observation that insulin-resistant people have lower palmitic acid esters of hydroxystearic acid (PAHSA) levels, suggesting that a FAHFA deficiency may contribute to metabolic disease. Rigorous testing of this hypothesis will require the measurement of many clinical samples; however, current analytical workflows are too slow to enable samples to be analyzed quickly. Here we describe the development of a significantly faster workflow to measure FAHFAs that optimizes the fractionation and chromatography of these lipids. We can measure FAHFAs in 30 min with this new protocol versus 90 min using the older protocol with comparable performance in regioisomer detection and quantitation. We also discovered through this optimization that oleic acid esters of hydroxystearic acids (OAHSAs), another family of FAHFAs, have a much lower background signal than PAHSAs, which makes them easier to measure. Our faster workflow was able to quantify changes in PAHSAs and OAHSAs in mouse tissues and human plasma, highlighting the potential of this protocol for basic and clinical applications.</p>}},
  author       = {{Kolar, Matthew J. and Nelson, Andrew T. and Chang, Tina and Ertunc, Meric Erikci and Christy, Mitchell P. and Ohlsson, Lena and Härröd, Magnus and Kahn, Barbara B. and Siegel, Dionicio and Saghatelian, Alan}},
  issn         = {{0003-2700}},
  language     = {{eng}},
  month        = {{04}},
  number       = {{8}},
  pages        = {{5358--5365}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Analytical Chemistry}},
  title        = {{Faster Protocol for Endogenous Fatty Acid Esters of Hydroxy Fatty Acid (FAHFA) Measurements}},
  url          = {{http://dx.doi.org/10.1021/acs.analchem.8b00503}},
  doi          = {{10.1021/acs.analchem.8b00503}},
  volume       = {{90}},
  year         = {{2018}},
}