Advanced

pH Tolerance in Freshwater Bacterioplankton: Trait-Variation of the Community measured by Leucine Incorporation.

Bååth, Erland LU and Kritzberg, Emma LU (2015) In Applied and Environmental Microbiology 81(21). p.7411-7419
Abstract
pH is an important factor determining bacterial community composition in soil and water. We have directly determined the community tolerance (trait variation) to pH in communities from 22 lakes and streams ranging in pH from 4 to 9 using a growth based method not relying on distinguishing between individual populations. pH in the water samples was altered to up to 16 pH values, covering in situ pH ± 2.5 units, and the tolerance was assessed by measuring bacterial growth (Leu incorporation) instantaneously after pH adjustment. The resulting unimodal response curves, reflecting community tolerance to pH, were well modeled with a double logistic equation (mean R(2) = 0.97). Optimal pH for growth (pHopt) among the bacterial communities was... (More)
pH is an important factor determining bacterial community composition in soil and water. We have directly determined the community tolerance (trait variation) to pH in communities from 22 lakes and streams ranging in pH from 4 to 9 using a growth based method not relying on distinguishing between individual populations. pH in the water samples was altered to up to 16 pH values, covering in situ pH ± 2.5 units, and the tolerance was assessed by measuring bacterial growth (Leu incorporation) instantaneously after pH adjustment. The resulting unimodal response curves, reflecting community tolerance to pH, were well modeled with a double logistic equation (mean R(2) = 0.97). Optimal pH for growth (pHopt) among the bacterial communities was closely correlated with in situ pH, with a slope (0.89 ± 0.099) close to unity. The pH interval, in which growth was ≥90 % of that at pHopt, was 1.1 to 3 pH units wide (mean 2.0 pH units). Tolerance response curves of communities originating from circum-neutral pH were symmetrical, while in high (pH 8.9) and especially low pH waters (pH<5.5) asymmetric tolerance curves were found. In low pH waters decreasing pH was more detrimental for bacterial growth than increasing pH, with a tendency for the opposite for high pH waters. A pH tolerance index, using the ratio of growth at only two pH values (pH 4 and 8), was closely related to pHopt (R(2) = 0.83), allowing for easy determination of pH tolerance during rapid changes in pH. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied and Environmental Microbiology
volume
81
issue
21
pages
7411 - 7419
publisher
American Society for Microbiology
external identifiers
  • pmid:26276108
  • wos:000363462900008
  • scopus:84944402361
ISSN
0099-2240
DOI
10.1128/AEM.02236-15
language
English
LU publication?
yes
id
12a85390-a3d5-43be-99a9-97ad32ee49da (old id 7841007)
date added to LUP
2015-09-11 13:20:17
date last changed
2017-07-09 03:00:14
@article{12a85390-a3d5-43be-99a9-97ad32ee49da,
  abstract     = {pH is an important factor determining bacterial community composition in soil and water. We have directly determined the community tolerance (trait variation) to pH in communities from 22 lakes and streams ranging in pH from 4 to 9 using a growth based method not relying on distinguishing between individual populations. pH in the water samples was altered to up to 16 pH values, covering in situ pH ± 2.5 units, and the tolerance was assessed by measuring bacterial growth (Leu incorporation) instantaneously after pH adjustment. The resulting unimodal response curves, reflecting community tolerance to pH, were well modeled with a double logistic equation (mean R(2) = 0.97). Optimal pH for growth (pHopt) among the bacterial communities was closely correlated with in situ pH, with a slope (0.89 ± 0.099) close to unity. The pH interval, in which growth was ≥90 % of that at pHopt, was 1.1 to 3 pH units wide (mean 2.0 pH units). Tolerance response curves of communities originating from circum-neutral pH were symmetrical, while in high (pH 8.9) and especially low pH waters (pH&lt;5.5) asymmetric tolerance curves were found. In low pH waters decreasing pH was more detrimental for bacterial growth than increasing pH, with a tendency for the opposite for high pH waters. A pH tolerance index, using the ratio of growth at only two pH values (pH 4 and 8), was closely related to pHopt (R(2) = 0.83), allowing for easy determination of pH tolerance during rapid changes in pH.},
  author       = {Bååth, Erland and Kritzberg, Emma},
  issn         = {0099-2240},
  language     = {eng},
  number       = {21},
  pages        = {7411--7419},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {pH Tolerance in Freshwater Bacterioplankton: Trait-Variation of the Community measured by Leucine Incorporation.},
  url          = {http://dx.doi.org/10.1128/AEM.02236-15},
  volume       = {81},
  year         = {2015},
}