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Supercritical Fluid Extraction and Chromatography of Lipids in Bilberry.

Jumaah, Firas LU ; Sandahl, Margareta LU and Turner, Charlotta LU (2015) In Journal of the American Oil Chemists Society 92(8). p.1103-1111
Abstract
A supercritical fluid extraction (SFE) method has been developed for the extraction of lipids in bilberry. Experimental design was used to optimize pressure, temperature and extraction time using CO2 as solvent. Best SFE condition for total lipids was 450 bar, 60 °C and 45 min. The SFE method was compared to conventional Bligh & Dyer (B&D) extraction. The amount of fatty acid methyl esters (FAME) was found to be 4.84 ± 0.06 mg and 4.564 ± 0.003 mg per g of the freeze-dried bilberry sample for the developed SFE and B&D methods, respectively, while the amount of total lipids was found to be 54.40 ± 6.06 mg and 65.70 ± 0.67 mg per g of sample for SFE and B&D, respectively. This discrepancy between FAME and total lipids could... (More)
A supercritical fluid extraction (SFE) method has been developed for the extraction of lipids in bilberry. Experimental design was used to optimize pressure, temperature and extraction time using CO2 as solvent. Best SFE condition for total lipids was 450 bar, 60 °C and 45 min. The SFE method was compared to conventional Bligh & Dyer (B&D) extraction. The amount of fatty acid methyl esters (FAME) was found to be 4.84 ± 0.06 mg and 4.564 ± 0.003 mg per g of the freeze-dried bilberry sample for the developed SFE and B&D methods, respectively, while the amount of total lipids was found to be 54.40 ± 6.06 mg and 65.70 ± 0.67 mg per g of sample for SFE and B&D, respectively. This discrepancy between FAME and total lipids could be explained by the presence of wax esters, sterol esters, carotenoids and phospholipids, as determined by supercritical fluid chromatography. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Journal of the American Oil Chemists Society
volume
92
issue
8
pages
1103 - 1111
publisher
The American Oil Chemists' Society
external identifiers
  • pmid:26246621
  • wos:000358781500004
  • scopus:84938509544
ISSN
0003-021X
DOI
10.1007/s11746-015-2680-x
language
English
LU publication?
yes
id
d31ce95b-edd3-473f-8c45-556b3ed82b10 (old id 7844573)
date added to LUP
2015-09-13 20:37:21
date last changed
2017-07-02 03:09:06
@article{d31ce95b-edd3-473f-8c45-556b3ed82b10,
  abstract     = {A supercritical fluid extraction (SFE) method has been developed for the extraction of lipids in bilberry. Experimental design was used to optimize pressure, temperature and extraction time using CO2 as solvent. Best SFE condition for total lipids was 450 bar, 60 °C and 45 min. The SFE method was compared to conventional Bligh & Dyer (B&D) extraction. The amount of fatty acid methyl esters (FAME) was found to be 4.84 ± 0.06 mg and 4.564 ± 0.003 mg per g of the freeze-dried bilberry sample for the developed SFE and B&D methods, respectively, while the amount of total lipids was found to be 54.40 ± 6.06 mg and 65.70 ± 0.67 mg per g of sample for SFE and B&D, respectively. This discrepancy between FAME and total lipids could be explained by the presence of wax esters, sterol esters, carotenoids and phospholipids, as determined by supercritical fluid chromatography.},
  author       = {Jumaah, Firas and Sandahl, Margareta and Turner, Charlotta},
  issn         = {0003-021X},
  language     = {eng},
  number       = {8},
  pages        = {1103--1111},
  publisher    = {The American Oil Chemists' Society},
  series       = {Journal of the American Oil Chemists Society},
  title        = {Supercritical Fluid Extraction and Chromatography of Lipids in Bilberry.},
  url          = {http://dx.doi.org/10.1007/s11746-015-2680-x},
  volume       = {92},
  year         = {2015},
}