Supercritical Fluid Extraction and Chromatography of Lipids in Bilberry.
(2015) In Journal of the American Oil Chemists Society 92(8). p.1103-1111- Abstract
- A supercritical fluid extraction (SFE) method has been developed for the extraction of lipids in bilberry. Experimental design was used to optimize pressure, temperature and extraction time using CO2 as solvent. Best SFE condition for total lipids was 450 bar, 60 °C and 45 min. The SFE method was compared to conventional Bligh & Dyer (B&D) extraction. The amount of fatty acid methyl esters (FAME) was found to be 4.84 ± 0.06 mg and 4.564 ± 0.003 mg per g of the freeze-dried bilberry sample for the developed SFE and B&D methods, respectively, while the amount of total lipids was found to be 54.40 ± 6.06 mg and 65.70 ± 0.67 mg per g of sample for SFE and B&D, respectively. This discrepancy between FAME and total lipids could... (More)
- A supercritical fluid extraction (SFE) method has been developed for the extraction of lipids in bilberry. Experimental design was used to optimize pressure, temperature and extraction time using CO2 as solvent. Best SFE condition for total lipids was 450 bar, 60 °C and 45 min. The SFE method was compared to conventional Bligh & Dyer (B&D) extraction. The amount of fatty acid methyl esters (FAME) was found to be 4.84 ± 0.06 mg and 4.564 ± 0.003 mg per g of the freeze-dried bilberry sample for the developed SFE and B&D methods, respectively, while the amount of total lipids was found to be 54.40 ± 6.06 mg and 65.70 ± 0.67 mg per g of sample for SFE and B&D, respectively. This discrepancy between FAME and total lipids could be explained by the presence of wax esters, sterol esters, carotenoids and phospholipids, as determined by supercritical fluid chromatography. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7844573
- author
- Jumaah, Firas LU ; Sandahl, Margareta LU and Turner, Charlotta LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of the American Oil Chemists Society
- volume
- 92
- issue
- 8
- pages
- 1103 - 1111
- publisher
- The American Oil Chemists' Society
- external identifiers
-
- pmid:26246621
- wos:000358781500004
- scopus:84938509544
- pmid:26246621
- ISSN
- 0003-021X
- DOI
- 10.1007/s11746-015-2680-x
- language
- English
- LU publication?
- yes
- id
- d31ce95b-edd3-473f-8c45-556b3ed82b10 (old id 7844573)
- date added to LUP
- 2016-04-01 10:15:36
- date last changed
- 2022-03-12 03:50:58
@article{d31ce95b-edd3-473f-8c45-556b3ed82b10,
abstract = {{A supercritical fluid extraction (SFE) method has been developed for the extraction of lipids in bilberry. Experimental design was used to optimize pressure, temperature and extraction time using CO2 as solvent. Best SFE condition for total lipids was 450 bar, 60 °C and 45 min. The SFE method was compared to conventional Bligh & Dyer (B&D) extraction. The amount of fatty acid methyl esters (FAME) was found to be 4.84 ± 0.06 mg and 4.564 ± 0.003 mg per g of the freeze-dried bilberry sample for the developed SFE and B&D methods, respectively, while the amount of total lipids was found to be 54.40 ± 6.06 mg and 65.70 ± 0.67 mg per g of sample for SFE and B&D, respectively. This discrepancy between FAME and total lipids could be explained by the presence of wax esters, sterol esters, carotenoids and phospholipids, as determined by supercritical fluid chromatography.}},
author = {{Jumaah, Firas and Sandahl, Margareta and Turner, Charlotta}},
issn = {{0003-021X}},
language = {{eng}},
number = {{8}},
pages = {{1103--1111}},
publisher = {{The American Oil Chemists' Society}},
series = {{Journal of the American Oil Chemists Society}},
title = {{Supercritical Fluid Extraction and Chromatography of Lipids in Bilberry.}},
url = {{http://dx.doi.org/10.1007/s11746-015-2680-x}},
doi = {{10.1007/s11746-015-2680-x}},
volume = {{92}},
year = {{2015}},
}