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A novel mechanism for NETosis provides antimicrobial defense at the oral mucosa.

Mohanty, Tirthankar LU ; Sjögren, Jonathan LU ; Kahn, Fredrik LU ; Abu-Humaidan, Anas LU ; Fisker, Niels; Assing, Kristian; Mörgelin, Matthias LU ; Bengtsson, Anders LU ; Borregaard, Niels and Sørensen, Ole E LU (2015) In Blood 126(18). p.2128-2137
Abstract
Neutrophils are essential for host defense at the oral mucosa and neutropenia or functional neutrophil defects lead to disordered oral homeostasis. We found that neutrophils from the oral mucosa harvested from morning saliva had undergone NETosis in vivo. The NETosis was mediated through intracellular signals elicited by binding of sialyl lewis(X) present on salival mucins to L-selectin on neutrophils. This led to rapid loss of nuclear membrane and intracellular release of granule proteins with subsequent NET release independent of elastase and NADPH-oxidase activation. The saliva-induced NETs were more DNase-resistant and had higher capacity to bind and kill bacteria than NETs induced by bacteria or by PMA. Furthermore, saliva/sialyl... (More)
Neutrophils are essential for host defense at the oral mucosa and neutropenia or functional neutrophil defects lead to disordered oral homeostasis. We found that neutrophils from the oral mucosa harvested from morning saliva had undergone NETosis in vivo. The NETosis was mediated through intracellular signals elicited by binding of sialyl lewis(X) present on salival mucins to L-selectin on neutrophils. This led to rapid loss of nuclear membrane and intracellular release of granule proteins with subsequent NET release independent of elastase and NADPH-oxidase activation. The saliva-induced NETs were more DNase-resistant and had higher capacity to bind and kill bacteria than NETs induced by bacteria or by PMA. Furthermore, saliva/sialyl lewis(X) mediated signaling enhanced intracellular killing of bacteria by neutrophils. Saliva from patients with aphthous ulcers and Behçet's disease prone to oral ulcers, failed to induce NETosis, but for different reasons, demonstrating that disordered homeostasis in the oral cavity may result in deficient saliva-mediated NETosis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Blood
volume
126
issue
18
pages
2128 - 2137
publisher
American Society of Hematology
external identifiers
  • pmid:26243777
  • wos:000366390100012
  • scopus:84945959235
ISSN
1528-0020
DOI
10.1182/blood-2015-04-641142
language
English
LU publication?
yes
id
ef844a75-201b-4cb1-a53b-c8490f17698a (old id 7844700)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26243777?dopt=Abstract
date added to LUP
2015-09-05 13:22:54
date last changed
2017-10-29 03:12:50
@article{ef844a75-201b-4cb1-a53b-c8490f17698a,
  abstract     = {Neutrophils are essential for host defense at the oral mucosa and neutropenia or functional neutrophil defects lead to disordered oral homeostasis. We found that neutrophils from the oral mucosa harvested from morning saliva had undergone NETosis in vivo. The NETosis was mediated through intracellular signals elicited by binding of sialyl lewis(X) present on salival mucins to L-selectin on neutrophils. This led to rapid loss of nuclear membrane and intracellular release of granule proteins with subsequent NET release independent of elastase and NADPH-oxidase activation. The saliva-induced NETs were more DNase-resistant and had higher capacity to bind and kill bacteria than NETs induced by bacteria or by PMA. Furthermore, saliva/sialyl lewis(X) mediated signaling enhanced intracellular killing of bacteria by neutrophils. Saliva from patients with aphthous ulcers and Behçet's disease prone to oral ulcers, failed to induce NETosis, but for different reasons, demonstrating that disordered homeostasis in the oral cavity may result in deficient saliva-mediated NETosis.},
  author       = {Mohanty, Tirthankar and Sjögren, Jonathan and Kahn, Fredrik and Abu-Humaidan, Anas and Fisker, Niels and Assing, Kristian and Mörgelin, Matthias and Bengtsson, Anders and Borregaard, Niels and Sørensen, Ole E},
  issn         = {1528-0020},
  language     = {eng},
  number       = {18},
  pages        = {2128--2137},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {A novel mechanism for NETosis provides antimicrobial defense at the oral mucosa.},
  url          = {http://dx.doi.org/10.1182/blood-2015-04-641142},
  volume       = {126},
  year         = {2015},
}