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Nucleolipid bilayers: A quartz crystal microbalance and neutron reflectometry study.

Montis, Costanza; Gerelli, Yuri; Fragneto, Giovanna; Nylander, Tommy LU ; Baglioni, Piero and Berti, Debora (2016) In Colloids and Surfaces B: Biointerfaces 137(Online 22 July 2015). p.203-213
Abstract
POP-Ade (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidyladenosine) is a biocompatible anionic nucleolipid with the DNA nucleoside, Adenosine, in the polar headgroup. We have studied the affinity of nucleic acids of different contour length, composition and structure toward supported lipid bilayers (SLB) composed of POP-Ade mixed with the zwitterionic phospholipid POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) using quartz crystal microbalance with dissipation monitoring (QCM-D) and neutron reflectometry (NR). In order to highlight the specificity of the nucleic acid interaction, the results were compared with data obtained for SLB containing the anionic phospholipid POPG (1-palmitoyl-2-oleoyl-sn-phosphatidyl-glycerol) replacing... (More)
POP-Ade (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidyladenosine) is a biocompatible anionic nucleolipid with the DNA nucleoside, Adenosine, in the polar headgroup. We have studied the affinity of nucleic acids of different contour length, composition and structure toward supported lipid bilayers (SLB) composed of POP-Ade mixed with the zwitterionic phospholipid POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) using quartz crystal microbalance with dissipation monitoring (QCM-D) and neutron reflectometry (NR). In order to highlight the specificity of the nucleic acid interaction, the results were compared with data obtained for SLB containing the anionic phospholipid POPG (1-palmitoyl-2-oleoyl-sn-phosphatidyl-glycerol) replacing POP-Ade. Our results demonstrate that the presence of a nucleobase headgroup provides the bilayers with the ability to bind single stranded nucleic acids in a selective fashion, according to a Watson-Crick pattern. In addition the interaction with double stranded nucleic acids was strengthened. Overall, these findings represent fundamental information for the design of biocompatible DNA vectors with DNA-RNA-based amphiphiles. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Colloids and Surfaces B: Biointerfaces
volume
137
issue
Online 22 July 2015
pages
203 - 213
publisher
Elsevier
external identifiers
  • pmid:26233685
  • wos:000367491200024
  • scopus:84951574200
ISSN
1873-4367
DOI
10.1016/j.colsurfb.2015.07.039
language
English
LU publication?
yes
id
a637791a-584d-4cb4-a0f7-eeb0fe42ce2e (old id 7845010)
date added to LUP
2015-09-13 20:30:09
date last changed
2017-02-05 03:03:21
@article{a637791a-584d-4cb4-a0f7-eeb0fe42ce2e,
  abstract     = {POP-Ade (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidyladenosine) is a biocompatible anionic nucleolipid with the DNA nucleoside, Adenosine, in the polar headgroup. We have studied the affinity of nucleic acids of different contour length, composition and structure toward supported lipid bilayers (SLB) composed of POP-Ade mixed with the zwitterionic phospholipid POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) using quartz crystal microbalance with dissipation monitoring (QCM-D) and neutron reflectometry (NR). In order to highlight the specificity of the nucleic acid interaction, the results were compared with data obtained for SLB containing the anionic phospholipid POPG (1-palmitoyl-2-oleoyl-sn-phosphatidyl-glycerol) replacing POP-Ade. Our results demonstrate that the presence of a nucleobase headgroup provides the bilayers with the ability to bind single stranded nucleic acids in a selective fashion, according to a Watson-Crick pattern. In addition the interaction with double stranded nucleic acids was strengthened. Overall, these findings represent fundamental information for the design of biocompatible DNA vectors with DNA-RNA-based amphiphiles.},
  author       = {Montis, Costanza and Gerelli, Yuri and Fragneto, Giovanna and Nylander, Tommy and Baglioni, Piero and Berti, Debora},
  issn         = {1873-4367},
  language     = {eng},
  number       = {Online 22 July 2015},
  pages        = {203--213},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces B: Biointerfaces},
  title        = {Nucleolipid bilayers: A quartz crystal microbalance and neutron reflectometry study.},
  url          = {http://dx.doi.org/10.1016/j.colsurfb.2015.07.039},
  volume       = {137},
  year         = {2016},
}