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Thousand-Fold Volumetric Concentration of Live Cells with a Recirculating Acoustofluidic Device.

Jakobsson, Ola LU ; Oh, Seung Soo; Antfolk, Maria LU ; Eisenstein, Michael; Laurell, Thomas LU and Soh, H Tom (2015) In Analytical Chemistry 87(16). p.8497-8502
Abstract
The ability to concentrate cells from dilute samples into smaller volumes is an essential process step for most biological assays. Volumetric concentration is typically achieved via centrifugation, but this technique is not well suited for handling small number of cells, especially outside of the laboratory setting. In this work, we describe a novel device that combines acoustofluidics with a recirculating architecture to achieve >1000-fold enrichment of cells in a label-free manner, at high volumetric throughput (>500 μL min(-1)) and with high recovery (>98.7%). We demonstrate that our device can be used with a wide variety of different cell types and show that this concentration strategy does not affect cell viability.... (More)
The ability to concentrate cells from dilute samples into smaller volumes is an essential process step for most biological assays. Volumetric concentration is typically achieved via centrifugation, but this technique is not well suited for handling small number of cells, especially outside of the laboratory setting. In this work, we describe a novel device that combines acoustofluidics with a recirculating architecture to achieve >1000-fold enrichment of cells in a label-free manner, at high volumetric throughput (>500 μL min(-1)) and with high recovery (>98.7%). We demonstrate that our device can be used with a wide variety of different cell types and show that this concentration strategy does not affect cell viability. Importantly, our device could be readily adopted to serve as a "sample preparation" module that can be integrated with other microfluidic devices to allow analysis of dilute cellular samples in large volumes. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
87
issue
16
pages
8497 - 8502
publisher
The American Chemical Society
external identifiers
  • wos:000359892100066
  • pmid:26226316
  • scopus:84939818134
ISSN
1520-6882
DOI
10.1021/acs.analchem.5b01944
language
English
LU publication?
yes
id
7f16e8fc-89b9-4c14-924e-369aad6d2f8e (old id 7850889)
date added to LUP
2015-09-14 10:08:12
date last changed
2017-11-05 03:26:48
@article{7f16e8fc-89b9-4c14-924e-369aad6d2f8e,
  abstract     = {The ability to concentrate cells from dilute samples into smaller volumes is an essential process step for most biological assays. Volumetric concentration is typically achieved via centrifugation, but this technique is not well suited for handling small number of cells, especially outside of the laboratory setting. In this work, we describe a novel device that combines acoustofluidics with a recirculating architecture to achieve >1000-fold enrichment of cells in a label-free manner, at high volumetric throughput (>500 μL min(-1)) and with high recovery (>98.7%). We demonstrate that our device can be used with a wide variety of different cell types and show that this concentration strategy does not affect cell viability. Importantly, our device could be readily adopted to serve as a "sample preparation" module that can be integrated with other microfluidic devices to allow analysis of dilute cellular samples in large volumes.},
  author       = {Jakobsson, Ola and Oh, Seung Soo and Antfolk, Maria and Eisenstein, Michael and Laurell, Thomas and Soh, H Tom},
  issn         = {1520-6882},
  language     = {eng},
  number       = {16},
  pages        = {8497--8502},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Thousand-Fold Volumetric Concentration of Live Cells with a Recirculating Acoustofluidic Device.},
  url          = {http://dx.doi.org/10.1021/acs.analchem.5b01944},
  volume       = {87},
  year         = {2015},
}