Thousand-Fold Volumetric Concentration of Live Cells with a Recirculating Acoustofluidic Device.
(2015) In Analytical Chemistry 87(16). p.8497-8502- Abstract
- The ability to concentrate cells from dilute samples into smaller volumes is an essential process step for most biological assays. Volumetric concentration is typically achieved via centrifugation, but this technique is not well suited for handling small number of cells, especially outside of the laboratory setting. In this work, we describe a novel device that combines acoustofluidics with a recirculating architecture to achieve >1000-fold enrichment of cells in a label-free manner, at high volumetric throughput (>500 μL min(-1)) and with high recovery (>98.7%). We demonstrate that our device can be used with a wide variety of different cell types and show that this concentration strategy does not affect cell viability.... (More)
- The ability to concentrate cells from dilute samples into smaller volumes is an essential process step for most biological assays. Volumetric concentration is typically achieved via centrifugation, but this technique is not well suited for handling small number of cells, especially outside of the laboratory setting. In this work, we describe a novel device that combines acoustofluidics with a recirculating architecture to achieve >1000-fold enrichment of cells in a label-free manner, at high volumetric throughput (>500 μL min(-1)) and with high recovery (>98.7%). We demonstrate that our device can be used with a wide variety of different cell types and show that this concentration strategy does not affect cell viability. Importantly, our device could be readily adopted to serve as a "sample preparation" module that can be integrated with other microfluidic devices to allow analysis of dilute cellular samples in large volumes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7850889
- author
- Jakobsson, Ola LU ; Oh, Seung Soo ; Antfolk, Maria LU ; Eisenstein, Michael ; Laurell, Thomas LU and Soh, H Tom
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 87
- issue
- 16
- pages
- 8497 - 8502
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000359892100066
- pmid:26226316
- scopus:84939818134
- pmid:26226316
- ISSN
- 1520-6882
- DOI
- 10.1021/acs.analchem.5b01944
- language
- English
- LU publication?
- yes
- id
- 7f16e8fc-89b9-4c14-924e-369aad6d2f8e (old id 7850889)
- date added to LUP
- 2016-04-01 11:15:49
- date last changed
- 2022-05-18 17:31:37
@article{7f16e8fc-89b9-4c14-924e-369aad6d2f8e,
abstract = {{The ability to concentrate cells from dilute samples into smaller volumes is an essential process step for most biological assays. Volumetric concentration is typically achieved via centrifugation, but this technique is not well suited for handling small number of cells, especially outside of the laboratory setting. In this work, we describe a novel device that combines acoustofluidics with a recirculating architecture to achieve >1000-fold enrichment of cells in a label-free manner, at high volumetric throughput (>500 μL min(-1)) and with high recovery (>98.7%). We demonstrate that our device can be used with a wide variety of different cell types and show that this concentration strategy does not affect cell viability. Importantly, our device could be readily adopted to serve as a "sample preparation" module that can be integrated with other microfluidic devices to allow analysis of dilute cellular samples in large volumes.}},
author = {{Jakobsson, Ola and Oh, Seung Soo and Antfolk, Maria and Eisenstein, Michael and Laurell, Thomas and Soh, H Tom}},
issn = {{1520-6882}},
language = {{eng}},
number = {{16}},
pages = {{8497--8502}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Analytical Chemistry}},
title = {{Thousand-Fold Volumetric Concentration of Live Cells with a Recirculating Acoustofluidic Device.}},
url = {{http://dx.doi.org/10.1021/acs.analchem.5b01944}},
doi = {{10.1021/acs.analchem.5b01944}},
volume = {{87}},
year = {{2015}},
}