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A validated LC-MS/MS method for the quantification of piperacillin/tazobactam on dried blood spot

Barco, Sebastiano; Risso, Francesco Maria; Bruschettini, Matteo LU ; Bandettini, Roberto; Ramenghi, Luca A.; Tripodi, Gino; Castagnola, Elio and Cangemi, Giuliana (2014) In Bioanalysis 6(21). p.2795-2802
Abstract
Background Therapeutic drug monitoring (TDM) of antibiotics in children is of fundamental importance for effective patient management. The use of dried blood spot (DBS) offers a number of advantages over conventional blood collection. The aim of this study is to develop and validate a method to measure piperacillin/tazobactam in DBS. Results The analysis was performed by using LC-MS/MS operating in multiple reaction monitoring mode. The method has been validated by applying EMA guidelines and its suitability for TDM was evaluated by using samples from low birth weight neonates. Conclusion This paper describes a fast and cost-effective micromethod for the simultaneous determination of piperacillin/tazobactam levels on dried blood spot that... (More)
Background Therapeutic drug monitoring (TDM) of antibiotics in children is of fundamental importance for effective patient management. The use of dried blood spot (DBS) offers a number of advantages over conventional blood collection. The aim of this study is to develop and validate a method to measure piperacillin/tazobactam in DBS. Results The analysis was performed by using LC-MS/MS operating in multiple reaction monitoring mode. The method has been validated by applying EMA guidelines and its suitability for TDM was evaluated by using samples from low birth weight neonates. Conclusion This paper describes a fast and cost-effective micromethod for the simultaneous determination of piperacillin/tazobactam levels on dried blood spot that is suitable for TDM in children. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioanalysis
volume
6
issue
21
pages
2795 - 2802
publisher
Future Science
external identifiers
  • wos:000346699600002
  • scopus:84918496205
ISSN
1757-6180
DOI
10.4155/BIO.14.205
language
English
LU publication?
no
id
0e4f2040-3a65-410d-b4bb-95ce30a7e813 (old id 7855465)
date added to LUP
2015-09-04 11:01:52
date last changed
2017-01-01 03:58:50
@article{0e4f2040-3a65-410d-b4bb-95ce30a7e813,
  abstract     = {Background Therapeutic drug monitoring (TDM) of antibiotics in children is of fundamental importance for effective patient management. The use of dried blood spot (DBS) offers a number of advantages over conventional blood collection. The aim of this study is to develop and validate a method to measure piperacillin/tazobactam in DBS. Results The analysis was performed by using LC-MS/MS operating in multiple reaction monitoring mode. The method has been validated by applying EMA guidelines and its suitability for TDM was evaluated by using samples from low birth weight neonates. Conclusion This paper describes a fast and cost-effective micromethod for the simultaneous determination of piperacillin/tazobactam levels on dried blood spot that is suitable for TDM in children.},
  author       = {Barco, Sebastiano and Risso, Francesco Maria and Bruschettini, Matteo and Bandettini, Roberto and Ramenghi, Luca A. and Tripodi, Gino and Castagnola, Elio and Cangemi, Giuliana},
  issn         = {1757-6180},
  language     = {eng},
  number       = {21},
  pages        = {2795--2802},
  publisher    = {Future Science},
  series       = {Bioanalysis},
  title        = {A validated LC-MS/MS method for the quantification of piperacillin/tazobactam on dried blood spot},
  url          = {http://dx.doi.org/10.4155/BIO.14.205},
  volume       = {6},
  year         = {2014},
}