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KIF5C S176 phosphorylation regulates microtubule binding and transport efficiency in mammalian neurons

Padzik, Artur; Deshpande, Prasannakumar; Hollos, Patrik; Franker, Mariella; Rannikko, Emmy H.; Cai, Dawen; Prus, Piotr; Mågård, Mats LU ; Westerlund, Nina and Verhey, Kristen J., et al. (2016) In Frontiers in Cellular Neuroscience 10(MAR2016).
Abstract

Increased phosphorylation of the KIF5 anterograde motor is associated with impaired axonal transport and neurodegeneration, but paradoxically also with normal transport, though the details are not fully defined. JNK phosphorylates KIF5C on S176 in the motor domain; a site that we show is phosphorylated in brain. Microtubule pelleting assays demonstrate that phosphomimetic KIF5C (1-560)S176D associates weakly with microtubules compared to KIF5C (1-560)WT. Consistent with this, 50% of KIF5C (1-560)S176D shows diffuse movement in neurons. However, the remaining 50% remains microtubule bound and displays decreased pausing and increased bidirectional movement. The same directionality switching is observed... (More)

Increased phosphorylation of the KIF5 anterograde motor is associated with impaired axonal transport and neurodegeneration, but paradoxically also with normal transport, though the details are not fully defined. JNK phosphorylates KIF5C on S176 in the motor domain; a site that we show is phosphorylated in brain. Microtubule pelleting assays demonstrate that phosphomimetic KIF5C (1-560)S176D associates weakly with microtubules compared to KIF5C (1-560)WT. Consistent with this, 50% of KIF5C (1-560)S176D shows diffuse movement in neurons. However, the remaining 50% remains microtubule bound and displays decreased pausing and increased bidirectional movement. The same directionality switching is observed with KIF5C(1-560)WT in the presence of an active JNK chimera, MKK7-JNK. Yet, in cargo trafficking assays where peroxisome cargo is bound, KIF5C(1-560)S176D-GFP-FRB transports normally to microtubule plus ends. We also find that JNK increases the ATP hydrolysis of KIF5C in vitro. These data suggest that phosphorylation of KIF5C-S176 primes the motor to either disengage entirely from microtubule tracks as previously observed in response to stress, or to display improved efficiency. The final outcome may depend on cargo load and motor ensembles.

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published
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Axonal transport, BDNF, JNK, Kinesin, Molecular motors, Neurons, Phosphorylation, SCG10
in
Frontiers in Cellular Neuroscience
volume
10
issue
MAR2016
publisher
Frontiers
external identifiers
  • Scopus:84961761048
  • WOS:000372107000001
ISSN
1662-5102
DOI
10.3389/fncel.2016.00057
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English
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yes
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787b1632-6d69-40d3-b2f7-24ff173b230f
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2016-09-19 10:47:24
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2017-01-01 08:34:15
@article{787b1632-6d69-40d3-b2f7-24ff173b230f,
  abstract     = {<p>Increased phosphorylation of the KIF5 anterograde motor is associated with impaired axonal transport and neurodegeneration, but paradoxically also with normal transport, though the details are not fully defined. JNK phosphorylates KIF5C on S176 in the motor domain; a site that we show is phosphorylated in brain. Microtubule pelleting assays demonstrate that phosphomimetic KIF5C (1-560)<sup>S176D</sup> associates weakly with microtubules compared to KIF5C (1-560)<sup>WT</sup>. Consistent with this, 50% of KIF5C (1-560)<sup>S176D</sup> shows diffuse movement in neurons. However, the remaining 50% remains microtubule bound and displays decreased pausing and increased bidirectional movement. The same directionality switching is observed with KIF5C(1-560)<sup>WT</sup> in the presence of an active JNK chimera, MKK7-JNK. Yet, in cargo trafficking assays where peroxisome cargo is bound, KIF5C(1-560)<sup>S176D</sup>-GFP-FRB transports normally to microtubule plus ends. We also find that JNK increases the ATP hydrolysis of KIF5C in vitro. These data suggest that phosphorylation of KIF5C-S176 primes the motor to either disengage entirely from microtubule tracks as previously observed in response to stress, or to display improved efficiency. The final outcome may depend on cargo load and motor ensembles.</p>},
  articleno    = {57},
  author       = {Padzik, Artur and Deshpande, Prasannakumar and Hollos, Patrik and Franker, Mariella and Rannikko, Emmy H. and Cai, Dawen and Prus, Piotr and Mågård, Mats and Westerlund, Nina and Verhey, Kristen J. and James, Peter and Hoogenraad, Casper C. and Coffey, Eleanor T.},
  issn         = {1662-5102},
  keyword      = {Axonal transport,BDNF,JNK,Kinesin,Molecular motors,Neurons,Phosphorylation,SCG10},
  language     = {eng},
  month        = {03},
  number       = {MAR2016},
  publisher    = {Frontiers},
  series       = {Frontiers in Cellular Neuroscience},
  title        = {KIF5C S176 phosphorylation regulates microtubule binding and transport efficiency in mammalian neurons},
  url          = {http://dx.doi.org/10.3389/fncel.2016.00057},
  volume       = {10},
  year         = {2016},
}