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Array transducer for ultrasonic manipulation of particles

Lilliehorn, Tobias; Johansson, Linda; Simu, Urban; Stepinski, Tadeusz; Johansson, Stefan; Evander, Mikael LU ; Almqvist, Monica LU ; Laurell, Thomas LU and Nilsson, Johan LU (2004) Micro Structure Workshop p.69-72
Abstract
This paper presents the concept and initial work on a microfluidic platform for bead-based analysis of biological sample. The core technology in this project is ultrasonic manipulation and trapping of particle in array configurations by means of acoustic forces. The platform is ultimately aimed for parallel multistep bioassays performed on biochemically activated microbeads (or particles) using submicrolitre sample volumes. A first prototype with three individually controlled particle trapping sites has been developed and evaluated. Standing ultrasonic waves were generated across a microfluidic channel by integrated PZT ultrasonic microtransducers. Particles in a fluid passing a transducer were drawn to pressure minima in the acoustic... (More)
This paper presents the concept and initial work on a microfluidic platform for bead-based analysis of biological sample. The core technology in this project is ultrasonic manipulation and trapping of particle in array configurations by means of acoustic forces. The platform is ultimately aimed for parallel multistep bioassays performed on biochemically activated microbeads (or particles) using submicrolitre sample volumes. A first prototype with three individually controlled particle trapping sites has been developed and evaluated. Standing ultrasonic waves were generated across a microfluidic channel by integrated PZT ultrasonic microtransducers. Particles in a fluid passing a transducer were drawn to pressure minima in the acoustic field, thereby being trapped and confined laterally over the transducer. It is anticipated that acoustic trapping using integrated transducers can be exploited in miniaturised total chemical analysis systems (µTAS), where e.g. microbeads with immobilised antibodies can be trapped in arrays and subjected to minute amounts of sample followed by a reaction, detected using fluorescence. A first model bioassay with detection of fluorescein marked avidin binding to trapped biotin beads has been evaluated. To enable development of the next generation of 2D array trapping devices, means of microfabricating multilayer ultrasonic array transducers using thick film technology have been developed. (Less)
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4 pages
conference name
Micro Structure Workshop
language
English
LU publication?
yes
id
e5f62b70-0be0-42ee-ae98-39bbb2030986 (old id 789619)
date added to LUP
2007-12-21 11:15:28
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2016-04-16 10:34:54
@misc{e5f62b70-0be0-42ee-ae98-39bbb2030986,
  abstract     = {This paper presents the concept and initial work on a microfluidic platform for bead-based analysis of biological sample. The core technology in this project is ultrasonic manipulation and trapping of particle in array configurations by means of acoustic forces. The platform is ultimately aimed for parallel multistep bioassays performed on biochemically activated microbeads (or particles) using submicrolitre sample volumes. A first prototype with three individually controlled particle trapping sites has been developed and evaluated. Standing ultrasonic waves were generated across a microfluidic channel by integrated PZT ultrasonic microtransducers. Particles in a fluid passing a transducer were drawn to pressure minima in the acoustic field, thereby being trapped and confined laterally over the transducer. It is anticipated that acoustic trapping using integrated transducers can be exploited in miniaturised total chemical analysis systems (µTAS), where e.g. microbeads with immobilised antibodies can be trapped in arrays and subjected to minute amounts of sample followed by a reaction, detected using fluorescence. A first model bioassay with detection of fluorescein marked avidin binding to trapped biotin beads has been evaluated. To enable development of the next generation of 2D array trapping devices, means of microfabricating multilayer ultrasonic array transducers using thick film technology have been developed.},
  author       = {Lilliehorn, Tobias and Johansson, Linda and Simu, Urban and Stepinski, Tadeusz and Johansson, Stefan and Evander, Mikael and Almqvist, Monica and Laurell, Thomas and Nilsson, Johan},
  language     = {eng},
  pages        = {69--72},
  title        = {Array transducer for ultrasonic manipulation of particles},
  year         = {2004},
}