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Versatile microchip utilising ultrasonic standing waves

Evander, Mikael LU ; Lilliehorn, Tobias; Johansson, Linda; Almqvist, Monica LU ; Simu, Urban; Johansson, Stefan; Laurell, Thomas LU and Nilsson, Johan LU (2005) 13th Nordic Baltic Conference on Biomedical Engineering and Medical Physics In IFMBE Proceedings 2005 p.123-124
Abstract
This paper presents the concept and initial work on a

microfluidic platform for bead-based analysis of

biological sample. The core technology in this project

is ultrasonic manipulation and trapping of particle in

array configurations by means of acoustic forces. The

platform is ultimately aimed for parallel multistep

bioassays performed on biochemically activated

microbeads (or particles) using submicrolitre sample

volumes. A first prototype with three individually

controlled particle trapping sites has been developed

and evaluated. Standing ultrasonic waves were

generated across a microfluidic channel by integrated

PZT ultrasonic... (More)
This paper presents the concept and initial work on a

microfluidic platform for bead-based analysis of

biological sample. The core technology in this project

is ultrasonic manipulation and trapping of particle in

array configurations by means of acoustic forces. The

platform is ultimately aimed for parallel multistep

bioassays performed on biochemically activated

microbeads (or particles) using submicrolitre sample

volumes. A first prototype with three individually

controlled particle trapping sites has been developed

and evaluated. Standing ultrasonic waves were

generated across a microfluidic channel by integrated

PZT ultrasonic microtransducers. Particles in a fluid

passing a transducer were drawn to pressure minima

in the acoustic field, thereby being trapped and

confined laterally over the transducer. It is

anticipated that acoustic trapping using integrated

transducers can be exploited in miniaturised total

chemical analysis systems (µTAS), where e.g.

microbeads with immobilised antibodies can be

trapped in arrays and subjected to minute amounts of

sample followed by a reaction, detected using

fluorescence. Preliminary results indicate that the

platform is capable of handling live cells as well as

microbeads. A first model bioassay with detection of

fluorescein marked avidin binding to trapped biotin

beads has been evaluated. (Less)
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publication status
published
subject
in
IFMBE Proceedings 2005
pages
2 pages
conference name
13th Nordic Baltic Conference on Biomedical Engineering and Medical Physics
language
English
LU publication?
yes
id
13ae3d63-a1ef-4bd5-ae0d-bed9f4e6a222 (old id 789762)
date added to LUP
2007-12-21 11:29:48
date last changed
2016-04-16 12:25:12
@inproceedings{13ae3d63-a1ef-4bd5-ae0d-bed9f4e6a222,
  abstract     = {This paper presents the concept and initial work on a<br/><br>
microfluidic platform for bead-based analysis of<br/><br>
biological sample. The core technology in this project<br/><br>
is ultrasonic manipulation and trapping of particle in<br/><br>
array configurations by means of acoustic forces. The<br/><br>
platform is ultimately aimed for parallel multistep<br/><br>
bioassays performed on biochemically activated<br/><br>
microbeads (or particles) using submicrolitre sample<br/><br>
volumes. A first prototype with three individually<br/><br>
controlled particle trapping sites has been developed<br/><br>
and evaluated. Standing ultrasonic waves were<br/><br>
generated across a microfluidic channel by integrated<br/><br>
PZT ultrasonic microtransducers. Particles in a fluid<br/><br>
passing a transducer were drawn to pressure minima<br/><br>
in the acoustic field, thereby being trapped and<br/><br>
confined laterally over the transducer. It is<br/><br>
anticipated that acoustic trapping using integrated<br/><br>
transducers can be exploited in miniaturised total<br/><br>
chemical analysis systems (µTAS), where e.g.<br/><br>
microbeads with immobilised antibodies can be<br/><br>
trapped in arrays and subjected to minute amounts of<br/><br>
sample followed by a reaction, detected using<br/><br>
fluorescence. Preliminary results indicate that the<br/><br>
platform is capable of handling live cells as well as<br/><br>
microbeads. A first model bioassay with detection of<br/><br>
fluorescein marked avidin binding to trapped biotin<br/><br>
beads has been evaluated.},
  author       = {Evander, Mikael and Lilliehorn, Tobias and Johansson, Linda and Almqvist, Monica and Simu, Urban and Johansson, Stefan and Laurell, Thomas and Nilsson, Johan},
  booktitle    = {IFMBE Proceedings 2005},
  language     = {eng},
  pages        = {123--124},
  title        = {Versatile microchip utilising ultrasonic standing waves},
  year         = {2005},
}