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Oxygen consumption of the aerobically-perfused cardioplegic donor heart at different temperatures

Qin, Guangqi LU ; Su, Yang; Sjöberg, Trygve LU and Steen, Stig LU (2018) In Annals of Transplantation 23. p.268-273
Abstract

Background: The aim of this study was to investigate the oxygen consumption of explanted aerobically-perfused cardioplegic porcine hearts at different temperatures. Material/Methods: Explanted hearts from 30 pigs weighing 50 kg were randomized into 5 groups. The hearts received continuous antegrade perfusion within a temperature-controlled sealed system. The perfusate consisted of an albumincontaining hyperoncotic cardioplegic nutrition solution with erythrocytes to a hematocrit of 10%. Five temperatures were studied: 37, 30, 22, 15, and 8°C. When the erythrocytes in the perfusate were fully saturated, the oxygenator was excluded from the circuit and blood gases were analyzed periodically until the erythrocytes had desaturated to less... (More)

Background: The aim of this study was to investigate the oxygen consumption of explanted aerobically-perfused cardioplegic porcine hearts at different temperatures. Material/Methods: Explanted hearts from 30 pigs weighing 50 kg were randomized into 5 groups. The hearts received continuous antegrade perfusion within a temperature-controlled sealed system. The perfusate consisted of an albumincontaining hyperoncotic cardioplegic nutrition solution with erythrocytes to a hematocrit of 10%. Five temperatures were studied: 37, 30, 22, 15, and 8°C. When the erythrocytes in the perfusate were fully saturated, the oxygenator was excluded from the circuit and blood gases were analyzed periodically until the erythrocytes had desaturated to less than 20%. Between 80% and 60% saturation the desaturation curves were linear in all groups and the oxygen consumption was calculated from this part of the curves. Results: The weight of the hearts was 208±4 g (mean ±SEM, n=30). The oxygen consumption in mL/min/100 g heart tissue was (mean ±SEM, n=6 in each group) 37°C: 1.10±0.04; 30°C: 0.58±0.02; 22°C: 0.33±0.01; 15°C: 0.21±0.01; and 8°C: 0.16±0.02. Conclusions: The oxygen consumption of the cardioplegic perfused pig heart at normothermia was 1.1 mL/min/100 g and was reduced by 85% to 0.16 mL/min/100 g at 8°C.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Heart, Metabolism, Organ preservation, Oxygen consumption, Perfusion
in
Annals of Transplantation
volume
23
pages
6 pages
publisher
ISI
external identifiers
  • scopus:85046160678
ISSN
1425-9524
DOI
10.12659/AOT.907753
language
English
LU publication?
yes
id
78c3220e-e851-46f8-a67b-5d5cfee31494
date added to LUP
2018-05-14 14:02:29
date last changed
2018-05-14 14:02:29
@article{78c3220e-e851-46f8-a67b-5d5cfee31494,
  abstract     = {<p>Background: The aim of this study was to investigate the oxygen consumption of explanted aerobically-perfused cardioplegic porcine hearts at different temperatures. Material/Methods: Explanted hearts from 30 pigs weighing 50 kg were randomized into 5 groups. The hearts received continuous antegrade perfusion within a temperature-controlled sealed system. The perfusate consisted of an albumincontaining hyperoncotic cardioplegic nutrition solution with erythrocytes to a hematocrit of 10%. Five temperatures were studied: 37, 30, 22, 15, and 8°C. When the erythrocytes in the perfusate were fully saturated, the oxygenator was excluded from the circuit and blood gases were analyzed periodically until the erythrocytes had desaturated to less than 20%. Between 80% and 60% saturation the desaturation curves were linear in all groups and the oxygen consumption was calculated from this part of the curves. Results: The weight of the hearts was 208±4 g (mean ±SEM, n=30). The oxygen consumption in mL/min/100 g heart tissue was (mean ±SEM, n=6 in each group) 37°C: 1.10±0.04; 30°C: 0.58±0.02; 22°C: 0.33±0.01; 15°C: 0.21±0.01; and 8°C: 0.16±0.02. Conclusions: The oxygen consumption of the cardioplegic perfused pig heart at normothermia was 1.1 mL/min/100 g and was reduced by 85% to 0.16 mL/min/100 g at 8°C.</p>},
  author       = {Qin, Guangqi and Su, Yang and Sjöberg, Trygve and Steen, Stig},
  issn         = {1425-9524},
  keyword      = {Heart,Metabolism,Organ preservation,Oxygen consumption,Perfusion},
  language     = {eng},
  month        = {04},
  pages        = {268--273},
  publisher    = {ISI},
  series       = {Annals of Transplantation},
  title        = {Oxygen consumption of the aerobically-perfused cardioplegic donor heart at different temperatures},
  url          = {http://dx.doi.org/10.12659/AOT.907753},
  volume       = {23},
  year         = {2018},
}