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AFM study of lipid monolayers. 2. Effect of cholesterol on fatty acids

Sparr, E. LU ; Ekelund, K. LU ; Engblom, J. LU ; Engström, S. and Wennerström, H. LU (1999) In Langmuir 15(20). p.6950-6955
Abstract

In this study the effect of cholesterol in Langmuir-Blodgett monolayers of fatty acids of varying chain lengths was investigated by atomic force microscopy (AFM). Domain formation due to lateral phase separation was studied at different lipid compositions and surface pressures. A small amount of cholesterol is miscible with palmitic acid (C16:0) and forms a flat monolayer while excess cholesterol forms a rougher cholesterol-rich phase. No miscibility was observed in monolayers of lignoceric acid (C24:0) and cholesterol. For the ternary mixed monolayer (palmitic acid, lignoceric acid, and cholesterol) the two fatty acids formed separate domains and the miscibility of cholesterol in the two phases showed behavior corresponding to that of... (More)

In this study the effect of cholesterol in Langmuir-Blodgett monolayers of fatty acids of varying chain lengths was investigated by atomic force microscopy (AFM). Domain formation due to lateral phase separation was studied at different lipid compositions and surface pressures. A small amount of cholesterol is miscible with palmitic acid (C16:0) and forms a flat monolayer while excess cholesterol forms a rougher cholesterol-rich phase. No miscibility was observed in monolayers of lignoceric acid (C24:0) and cholesterol. For the ternary mixed monolayer (palmitic acid, lignoceric acid, and cholesterol) the two fatty acids formed separate domains and the miscibility of cholesterol in the two phases showed behavior corresponding to that of the binary fatty acid-cholesterol systems. From the shape, size, and height differences of the domains one can conclude that the driving force to minimize the interfacial length between different phases is reduced in the presence of cholesterol. This can be attributed to line active properties of cholesterol.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
15
issue
20
pages
6 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:0032685678
ISSN
0743-7463
DOI
10.1021/la9900932
language
English
LU publication?
yes
id
79430714-827a-4ff7-9c92-66f834a3f684
date added to LUP
2023-12-07 14:59:56
date last changed
2023-12-21 23:46:04
@article{79430714-827a-4ff7-9c92-66f834a3f684,
  abstract     = {{<p>In this study the effect of cholesterol in Langmuir-Blodgett monolayers of fatty acids of varying chain lengths was investigated by atomic force microscopy (AFM). Domain formation due to lateral phase separation was studied at different lipid compositions and surface pressures. A small amount of cholesterol is miscible with palmitic acid (C16:0) and forms a flat monolayer while excess cholesterol forms a rougher cholesterol-rich phase. No miscibility was observed in monolayers of lignoceric acid (C24:0) and cholesterol. For the ternary mixed monolayer (palmitic acid, lignoceric acid, and cholesterol) the two fatty acids formed separate domains and the miscibility of cholesterol in the two phases showed behavior corresponding to that of the binary fatty acid-cholesterol systems. From the shape, size, and height differences of the domains one can conclude that the driving force to minimize the interfacial length between different phases is reduced in the presence of cholesterol. This can be attributed to line active properties of cholesterol.</p>}},
  author       = {{Sparr, E. and Ekelund, K. and Engblom, J. and Engström, S. and Wennerström, H.}},
  issn         = {{0743-7463}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{20}},
  pages        = {{6950--6955}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Langmuir}},
  title        = {{AFM study of lipid monolayers. 2. Effect of cholesterol on fatty acids}},
  url          = {{http://dx.doi.org/10.1021/la9900932}},
  doi          = {{10.1021/la9900932}},
  volume       = {{15}},
  year         = {{1999}},
}