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Early apoptotic response in kidney after 177Lu-octreotate administration with or without potential radioprotector α1-microglobulin

Ytterbrink, Charlotte ; Simonsson, Klara ; Shubbar, Emman ; Gram, Magnus LU orcid ; Helou, Khalil and Forssell-Aronsson, Eva (2025) In Radiation Protection Dosimetry 201(13-14). p.877-886
Abstract

The antioxidant α1-microglobulin (A1M) has been suggested as kidney protector during 177Lu-octreotate treatment. The aim of this work was to evaluate apoptotic-related transcript expression in kidney cortex and medulla following injection of 177Lu-octreotate and/or A1M. Mice were injected with 177Lu-octreotate, A1M, or 177Lu-octreotate + A1M. Control groups received PBS or vehicle solution. Animals were killed after 24 hours or 7 d. mRNA was isolated from kidney medulla and cortex. Expression of 84 apoptosis-related genes was assessed by q-PCR. Gene expression profiles in kidney cortex were generally similar in the 177Lu-octreotate and 177Lu-octreotate + A1M... (More)

The antioxidant α1-microglobulin (A1M) has been suggested as kidney protector during 177Lu-octreotate treatment. The aim of this work was to evaluate apoptotic-related transcript expression in kidney cortex and medulla following injection of 177Lu-octreotate and/or A1M. Mice were injected with 177Lu-octreotate, A1M, or 177Lu-octreotate + A1M. Control groups received PBS or vehicle solution. Animals were killed after 24 hours or 7 d. mRNA was isolated from kidney medulla and cortex. Expression of 84 apoptosis-related genes was assessed by q-PCR. Gene expression profiles in kidney cortex were generally similar in the 177Lu-octreotate and 177Lu-octreotate + A1M groups. This was also seen in kidney medulla at 24 hours, but at 7 d anti-apoptotic response of A1M was observed. Altogether, 177Lu-octreotate exposure induced pro-apoptotic response (e.g. Apaf1, Bax, and Tnfrsf10b genes) in kidney medulla and cortex. A1M co-administration did not inhibit pro-apoptotic response in kidney cortex, while A1M initiated pro-survival mechanisms in kidney medulla.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Radiation Protection Dosimetry
volume
201
issue
13-14
pages
10 pages
publisher
Oxford University Press
external identifiers
  • pmid:40875262
  • scopus:105014592345
ISSN
0144-8420
DOI
10.1093/rpd/ncaf055
language
English
LU publication?
yes
id
794dc717-49e5-49a3-9407-9090ff493cfb
date added to LUP
2025-10-16 15:39:31
date last changed
2025-10-17 04:03:13
@article{794dc717-49e5-49a3-9407-9090ff493cfb,
  abstract     = {{<p>The antioxidant α<sub>1</sub>-microglobulin (A1M) has been suggested as kidney protector during <sup>177</sup>Lu-octreotate treatment. The aim of this work was to evaluate apoptotic-related transcript expression in kidney cortex and medulla following injection of <sup>177</sup>Lu-octreotate and/or A1M. Mice were injected with <sup>177</sup>Lu-octreotate, A1M, or <sup>177</sup>Lu-octreotate + A1M. Control groups received PBS or vehicle solution. Animals were killed after 24 hours or 7 d. mRNA was isolated from kidney medulla and cortex. Expression of 84 apoptosis-related genes was assessed by q-PCR. Gene expression profiles in kidney cortex were generally similar in the <sup>177</sup>Lu-octreotate and <sup>177</sup>Lu-octreotate + A1M groups. This was also seen in kidney medulla at 24 hours, but at 7 d anti-apoptotic response of A1M was observed. Altogether, <sup>177</sup>Lu-octreotate exposure induced pro-apoptotic response (e.g. Apaf1, Bax, and Tnfrsf10b genes) in kidney medulla and cortex. A1M co-administration did not inhibit pro-apoptotic response in kidney cortex, while A1M initiated pro-survival mechanisms in kidney medulla.</p>}},
  author       = {{Ytterbrink, Charlotte and Simonsson, Klara and Shubbar, Emman and Gram, Magnus and Helou, Khalil and Forssell-Aronsson, Eva}},
  issn         = {{0144-8420}},
  language     = {{eng}},
  number       = {{13-14}},
  pages        = {{877--886}},
  publisher    = {{Oxford University Press}},
  series       = {{Radiation Protection Dosimetry}},
  title        = {{Early apoptotic response in kidney after <sup>177</sup>Lu-octreotate administration with or without potential radioprotector α<sub>1</sub>-microglobulin}},
  url          = {{http://dx.doi.org/10.1093/rpd/ncaf055}},
  doi          = {{10.1093/rpd/ncaf055}},
  volume       = {{201}},
  year         = {{2025}},
}