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Protein C inhibitor in human body fluids. Seminal plasma is rich in inhibitor antigen deriving from cells throughout the male reproductive system

Laurell, M LU ; Christensson, A LU ; Abrahamsson, P A LU ; Stenflo, J LU and Lilja, H LU (1992) In Journal of Clinical Investigation 89(4). p.101-1094
Abstract

An assay was developed for the measurement of human protein C inhibitor antigen (PCI) in blood plasma and other biological fluids. Both native PCI, modified inhibitor, and complexes of inhibitor with activated protein C or plasma kallikrein could be measured with the assay. Inhibitor antigen concentrations were found to be very high in seminal plasma (greater than 200 mg/liter), more than 40 times the concentration of PCI found in blood plasma. The inhibitor in seminal plasma was unable to form complexes with activated protein C. Gel filtration and immunoblotting findings indicated that the inhibitor in seminal plasma is present in a high molecular mass complex or cleaved to its modified form. As PCI antigen was absent from seminal... (More)

An assay was developed for the measurement of human protein C inhibitor antigen (PCI) in blood plasma and other biological fluids. Both native PCI, modified inhibitor, and complexes of inhibitor with activated protein C or plasma kallikrein could be measured with the assay. Inhibitor antigen concentrations were found to be very high in seminal plasma (greater than 200 mg/liter), more than 40 times the concentration of PCI found in blood plasma. The inhibitor in seminal plasma was unable to form complexes with activated protein C. Gel filtration and immunoblotting findings indicated that the inhibitor in seminal plasma is present in a high molecular mass complex or cleaved to its modified form. As PCI antigen was absent from seminal plasma of patients with dysfunctional seminal vesicles, the seminal vesicle glands would appear to be the major source of seminal plasma PCI, a conclusion supported by immunohistochemical demonstration of the presence of PCI epitopes in the secretory epithelium of the seminal vesicles. Specific PCI immunoreactivity was also shown to be present in the testes, the epididymis glands, and the prostate, suggesting the inhibitor to have a complex or multiple function in the male reproductive system. Conclusive evidence of a local synthesis of PCI in the four male sex glands was provided by Northern blot analysis of RNA from these organs.

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keywords
Adolescent, Adult, Aged, Antigens/analysis, Body Fluids/chemistry, Epitopes/analysis, Female, Humans, Male, Middle Aged, Molecular Weight, Protein C/antagonists & inhibitors, Radioimmunoassay, Semen/chemistry, Serine Proteinase Inhibitors/analysis
in
Journal of Clinical Investigation
volume
89
issue
4
pages
8 pages
publisher
Am Soc Clin Investig
external identifiers
  • scopus:0026654795
ISSN
0021-9738
DOI
10.1172/JCI115689
language
English
LU publication?
yes
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796b812c-ceac-471c-9d02-dfc51f238783
date added to LUP
2019-05-16 14:07:47
date last changed
2019-10-13 05:01:48
@article{796b812c-ceac-471c-9d02-dfc51f238783,
  abstract     = {<p>An assay was developed for the measurement of human protein C inhibitor antigen (PCI) in blood plasma and other biological fluids. Both native PCI, modified inhibitor, and complexes of inhibitor with activated protein C or plasma kallikrein could be measured with the assay. Inhibitor antigen concentrations were found to be very high in seminal plasma (greater than 200 mg/liter), more than 40 times the concentration of PCI found in blood plasma. The inhibitor in seminal plasma was unable to form complexes with activated protein C. Gel filtration and immunoblotting findings indicated that the inhibitor in seminal plasma is present in a high molecular mass complex or cleaved to its modified form. As PCI antigen was absent from seminal plasma of patients with dysfunctional seminal vesicles, the seminal vesicle glands would appear to be the major source of seminal plasma PCI, a conclusion supported by immunohistochemical demonstration of the presence of PCI epitopes in the secretory epithelium of the seminal vesicles. Specific PCI immunoreactivity was also shown to be present in the testes, the epididymis glands, and the prostate, suggesting the inhibitor to have a complex or multiple function in the male reproductive system. Conclusive evidence of a local synthesis of PCI in the four male sex glands was provided by Northern blot analysis of RNA from these organs.</p>},
  author       = {Laurell, M and Christensson, A and Abrahamsson, P A and Stenflo, J and Lilja, H},
  issn         = {0021-9738},
  keyword      = {Adolescent,Adult,Aged,Antigens/analysis,Body Fluids/chemistry,Epitopes/analysis,Female,Humans,Male,Middle Aged,Molecular Weight,Protein C/antagonists & inhibitors,Radioimmunoassay,Semen/chemistry,Serine Proteinase Inhibitors/analysis},
  language     = {eng},
  number       = {4},
  pages        = {101--1094},
  publisher    = {Am Soc Clin Investig},
  series       = {Journal of Clinical Investigation},
  title        = {Protein C inhibitor in human body fluids. Seminal plasma is rich in inhibitor antigen deriving from cells throughout the male reproductive system},
  url          = {http://dx.doi.org/10.1172/JCI115689},
  volume       = {89},
  year         = {1992},
}